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A6WMW5 (GLO2_SHEB8) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 34. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Hydroxyacylglutathione hydrolase
EC=3.1.2.6
Alternative name(s):
Glyoxalase II
Short name=Glx II
Gene names
Name:gloB
Ordered Locus Names:Shew185_2011
OrganismShewanella baltica (strain OS185) [Complete proteome] [HAMAP]
Taxonomic identifier402882 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length263 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374

Catalytic activity

S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374

Cofactor

Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374

Pathway

Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374

Subunit structure

Monomer By similarity. HAMAP MF_01374

Sequence similarities

Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

Ontologies

Keywords
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular functionhydroxyacylglutathione hydrolase activity

Inferred from electronic annotation. Source: EC

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 263263Hydroxyacylglutathione hydrolase HAMAP MF_01374
PRO_1000144804

Sites

Metal binding551Zinc 1 By similarity
Metal binding571Zinc 1 By similarity
Metal binding591Zinc 2 By similarity
Metal binding601Zinc 2 By similarity
Metal binding1171Zinc 1 By similarity
Metal binding1341Zinc 1 By similarity
Metal binding1341Zinc 2 By similarity
Metal binding1721Zinc 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A6WMW5 [UniParc].

Last modified August 21, 2007. Version 1.
Checksum: 6E3D568BB7B9504E

FASTA26329,236
        10         20         30         40         50         60 
MLTITAIKAF NDNYIWVLQQ QPHTQVYVVD PGDASVVIDY LEANKLTLAG ILLTHHHNDH 

        70         80         90        100        110        120 
TGGVAELQAY SQNRLSVYGP DNEKIEGITH PLHATAQPRF ILDYMSGELQ VLDVPGHTAG 

       130        140        150        160        170        180 
HIAYVIADAL FCGDTLFSGG CGRLFEGTPA QMLNSLQQLA QLPADTRVYC AHEYTLSNLK 

       190        200        210        220        230        240 
FALAVNPNNR ALQDYNERAI ALRRQEKATI PSTIALERAI NPFLRASDTE IVDSIKQHFS 

       250        260 
DLNHANLDEL GGFTLLRQWK DNF

« Hide

References

[1]"Complete sequence of chromosome of Shewanella baltica OS185."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Mikhailova N., Brettar I., Rodrigues J., Konstantinidis K., Tiedje J., Richardson P.
Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: OS185.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000753 Genomic DNA. Translation: ABS08154.1.
RefSeqYP_001366217.1. NC_009665.1.

3D structure databases

ProteinModelPortalA6WMW5.
ModBaseSearch...

Protein-protein interaction databases

STRINGA6WMW5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5373372.
GenomeReviewsGene locus Shew185_2011 in contig CP000753_GR.
KEGGsbm:Shew185_2011.
PATRIC23460770. VBISheBal127872_2204.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0491.
HOGENOMHBG753931.
OMAQEYAANA.
ProtClustDBCLSK906771.

Enzyme and pathway databases

BioCycSBAL402882:SHEW185_2011-MONOMER.

Family and domain databases

HAMAPMF_01374. Glyoxalase_2.
[Tree]
InterProIPR001279. Beta-lactamas-like.
IPR017782. Hydroxyacylglutathione_Hdrlase.
[Graphical view]
KOK01069.
PANTHERPTHR11935:SF7. PTHR11935:SF7. 1 hit.
PfamPF00753. Lactamase_B. 1 hit.
[Graphical view]
SMARTSM00849. Lactamase_B. 1 hit.
[Graphical view]
TIGRFAMsTIGR03413. GSH_gloB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLO2_SHEB8
AccessionPrimary (citable) accession number: A6WMW5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: August 21, 2007
Last modified: January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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