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A6WM55

- BIOB_SHEB8

UniProt

A6WM55 - BIOB_SHEB8

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Protein

Biotin synthase

Gene
bioB, Shew185_1751
Organism
Shewanella baltica (strain OS185)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi56 – 561Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi60 – 601Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi63 – 631Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi100 – 1001Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi131 – 1311Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi191 – 1911Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi263 – 2631Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSBAL402882:GJ99-1798-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:Shew185_1751
OrganismiShewanella baltica (strain OS185)
Taxonomic identifieri402882 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000006387: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 350350Biotin synthaseUniRule annotationPRO_0000381615Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi402882.Shew185_1751.

Structurei

3D structure databases

ProteinModelPortaliA6WM55.
SMRiA6WM55. Positions 7-317.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiRIMMPAS.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

A6WM55-1 [UniParc]FASTAAdd to Basket

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MSQLQVRHDW KREEIEALFA LPMNDLLFKA HSIHREVYDP NEVQISRLLS    50
IKTGACPEDC KYCPQSARYD TGLEKERLLA METVLTEARS AKAAGASRFC 100
MGAAWRNPKE KDMPYLKQMV QEVKALGMET CMTLGMLSED QANDLASAGL 150
DYYNHNLDTS PEYYGDVITT RTYQNRLDTL TNVRASGMKV CSGGIVGMGE 200
KATDRAGLLQ QLANLPQHPD SVPINMLVKV AGTPFEKLDD LDPLEFVRTI 250
AVARILMPLS RVRLSAGREN MSDELQAMCF FAGANSIFYG CKLLTTPNPE 300
ESDDMGLFRR LGLRPEQGAA AKLEEESAVL AKAAAYQDKS SAQFYDAGAL 350
Length:350
Mass (Da):38,783
Last modified:August 21, 2007 - v1
Checksum:i9B6A317D5553E1AC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000753 Genomic DNA. Translation: ABS07894.1.
RefSeqiYP_001365957.1. NC_009665.1.

Genome annotation databases

EnsemblBacteriaiABS07894; ABS07894; Shew185_1751.
GeneIDi5371543.
KEGGisbm:Shew185_1751.
PATRICi23460222. VBISheBal127872_1931.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000753 Genomic DNA. Translation: ABS07894.1 .
RefSeqi YP_001365957.1. NC_009665.1.

3D structure databases

ProteinModelPortali A6WM55.
SMRi A6WM55. Positions 7-317.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 402882.Shew185_1751.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABS07894 ; ABS07894 ; Shew185_1751 .
GeneIDi 5371543.
KEGGi sbm:Shew185_1751.
PATRICi 23460222. VBISheBal127872_1931.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi RIMMPAS.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci SBAL402882:GJ99-1798-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: OS185.

Entry informationi

Entry nameiBIOB_SHEB8
AccessioniPrimary (citable) accession number: A6WM55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: August 21, 2007
Last modified: May 14, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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