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A6WKN2 (MTNN_SHEB8) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 31. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
Short name=MTA/SAH nucleosidase
Short name=MTAN
EC=3.2.2.9
Alternative name(s):
5'-methylthioadenosine nucleosidase
Short name=MTA nucleosidase
S-adenosylhomocysteine nucleosidase
Short name=AdoHcy nucleosidase
Short name=SAH nucleosidase
Short name=SRH nucleosidase
Gene names
Name:mtnN
Ordered Locus Names:Shew185_1220
OrganismShewanella baltica (strain OS185) [Complete proteome] [HAMAP]
Taxonomic identifier402882 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length236 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively By similarity. HAMAP MF_01684

Catalytic activity

S-adenosyl-L-homocysteine + H2O = S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine. HAMAP MF_01684

S-methyl-5'-thioadenosine + H2O = S-methyl-5-thio-D-ribose + adenine. HAMAP MF_01684

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (hydrolase route): step 1/2. HAMAP MF_01684

Sequence similarities

Belongs to the PNP/UDP phosphorylase family. MtnN subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2362365'-methylthioadenosine/S-adenosylhomocysteine nucleosidase HAMAP MF_01684
PRO_0000359341

Regions

Region174 – 1752Substrate binding By similarity

Sites

Active site121Proton acceptor By similarity
Binding site781Substrate; via amide nitrogen By similarity
Binding site1531Substrate; via amide nitrogen and carbonyl oxygen By similarity
Binding site1981Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
A6WKN2 [UniParc].

Last modified August 21, 2007. Version 1.
Checksum: 92F6E26DC3D1DA61

FASTA23624,644
        10         20         30         40         50         60 
MKIGIIGAME PEVAHLIAAM TNATSQTIAG IEFIAGTLAG KDVVVTRSGI GKVAASIATT 

        70         80         90        100        110        120 
LLIEKYAPDA VINTGSAGGF VDTLAIGDIV ISSEVRHHDV DVTAFGYEIG QMAQQPAAFV 

       130        140        150        160        170        180 
PAAHLVEAAN KAIAQLGEVK AIEGLICTGD SFICDPIRTQ AMLKNFPTMA ACEMEGAAIA 

       190        200        210        220        230 
QVCHQFGVPF VVIRSLSDNA NNDSPVDFDS YIVKAGYHSA LMVMLLLEQL NPSAIK

« Hide

References

[1]"Complete sequence of chromosome of Shewanella baltica OS185."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Mikhailova N., Brettar I., Rodrigues J., Konstantinidis K., Tiedje J., Richardson P.
Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: OS185.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000753 Genomic DNA. Translation: ABS07371.1.
RefSeqYP_001365434.1. NC_009665.1.

3D structure databases

ProteinModelPortalA6WKN2.
ModBaseSearch...

Protein-protein interaction databases

STRINGA6WKN2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5373527.
GenomeReviewsGene locus Shew185_1220 in contig CP000753_GR.
KEGGsbm:Shew185_1220.
PATRIC23459099. VBISheBal127872_1380.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0775.
HOGENOMHBG367723.
OMALEHFPTM.
ProtClustDBCLSK906203.

Enzyme and pathway databases

BioCycSBAL402882:SHEW185_1220-MONOMER.

Family and domain databases

HAMAPMF_01684. Salvage_tnN.
[Tree]
InterProIPR010049. MTA_SAH_Nsdase.
IPR018017. Nucleoside_phosphorylase.
IPR000845. Nucleoside_phosphorylase_d.
[Graphical view]
KOK01243.
PANTHERPTHR21234. PNP_UDP. 1 hit.
PTHR21234:SF6. PTHR21234:SF6. 1 hit.
PfamPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
TIGRFAMsTIGR01704. MTA/SAH-Nsdase. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMTNN_SHEB8
AccessionPrimary (citable) accession number: A6WKN2
Entry history
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: August 21, 2007
Last modified: January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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