A6WGF9 (TYSY_KINRD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	Krad_4439

Organism

Kineococcus radiotolerans (strain ATCC BAA-149 / DSM 14245 / SRS30216) [Complete proteome] [HAMAP]

Taxonomic identifier

266940 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Kineosporiineae › Kineosporiaceae › Kineococcus

Protein attributes

Sequence length	268 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008
Subunit structure	Homodimer By similarity. HAMAP MF_00008
Subcellular location	Cytoplasm By similarity HAMAP MF_00008.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 268	268	Thymidylate synthase HAMAP MF_00008		PRO_0000336006
Sites
	Active site	152	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6WGF9 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 0AAC4AA8E89152DB	FASTA	268	30,504
	10 20 30 40 50 60 MAEQRIDTQY EDLLRHVLAT GTPKSDRTGT GTRSVFGHQL RYDLSAGFPL VTTKRVHFKS 70 80 90 100 110 120 IALELLWFLR GDGNVRWLQE RGVTIWDEWA DENGDLGPVY GVQWRSWPTP DGRHVDQIAQ 130 140 150 160 170 180 VLHTLRTDPD SRRMVVSAWN VAELDRMALA PCHAFFQFHV ADGKLSCQLY QRSADLFLGV 190 200 210 220 230 240 PFNIASYALL THLVAAEVGL EVGDFVWTGG DVHVYDNHVE QVTEQLTRTP YPFPRLAVRP 250 260 AGLFEHEYED FEVLDYRHHP AIKAPVAV

References

[1]

"Complete sequence of chromosome of Kineococcus radiotolerans SRS30216." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Lykidis A., Bagwell C.E., Shimkets L., Berry C.J., Fliermans C., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-149 / DSM 14245 / SRS30216.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000750 Genomic DNA. Translation: ABS05898.1.
RefSeq	YP_001364162.1. NC_009664.2.
3D structure databases
ProteinModelPortal	A6WGF9.
SMR	A6WGF9. Positions 9-268.
ModBase	Search...
Protein-protein interaction databases
STRING	A6WGF9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5333994.
GenomeReviews	Gene locus Krad_4439 in contig CP000750_GR.
KEGG	kra:Krad_4439.
NMPDR	fig|266940.1.peg.2505.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0207.
HOGENOM	HBG588098.
OMA	RMALAPC.
Enzyme and pathway databases
BioCyc	KRAD266940:KRAD_4439-MONOMER.
Family and domain databases
HAMAP	MF_00008. Thymidy_synth_bact. [Tree]
InterPro	IPR023451. Thymidate_synth/dCMP_Mease. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
KO	K00560.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
SUPFAM	SSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMs	TIGR03284. Thym_sym. 2 hits.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_KINRD

Accession

Primary (citable) accession number: A6WGF9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	August 21, 2007
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program