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A6WDL7 (SYL_KINRD) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Krad_3443
OrganismKineococcus radiotolerans (strain ATCC BAA-149 / DSM 14245 / SRS30216) [Complete proteome] [HAMAP]
Taxonomic identifier266940 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesKineosporiineaeKineosporiaceaeKineococcus

Protein attributes

Sequence length855 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 855855Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334765

Regions

Motif71 – 8111"HIGH" region HAMAP-Rule MF_00049
Motif620 – 6245"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6231ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A6WDL7 [UniParc].

Last modified August 21, 2007. Version 1.
Checksum: 1FC3E953BCA4D622

FASTA85594,721
        10         20         30         40         50         60 
MRDARTHRED EMTQTDTQAT GPDRAEEQDG QRYDAFALQE KWLPVWDERK PFRSGEPGDE 

        70         80         90        100        110        120 
RPKKYVLDMF PYPSGDLHMG HAEAYALGDV IARYWLQRGF DVMHPIGWDA FGLPAENAAI 

       130        140        150        160        170        180 
KRGLDPRRWT YDNIAQQRAS MRRYACSFDW DRVLHTSDPE YYRWNQWLFL KLYEKGLAYR 

       190        200        210        220        230        240 
KDSLVNWDPV DQTVLANEQV LPDGTSERSG AKVVKKKLTQ WYFRITEYAD RLLDDLDTLE 

       250        260        270        280        290        300 
GQWPAKVISM QRNWIGRSTG AEVEFAIEGR DEPVTVYTTR PDTLHGATFM VVAADSDLAE 

       310        320        330        340        350        360 
ELAAGAPDDV RAAFEAYRTQ VQETSDIDRL SSEREKTGVP LGRYAVNPVN GERLPIWAAD 

       370        380        390        400        410        420 
YVLADYGTGA IMAVPAHDQR DLDFARAFGL PVRVVVDVRD DDGNPLPDPA ESGTATAGDG 

       430        440        450        460        470        480 
VLVNSGALDG LGKQAGIAKA IEDLTAAGKG RAAKNYRLRD WLISRQRYWG TPIPVVHTEN 

       490        500        510        520        530        540 
GEVPVPEDQL PVLLPPSEGL NLTPKGTSPL GAAEDWVNVP SPVDGSPARR DPDTMDTFVD 

       550        560        570        580        590        600 
SSWYFLRFVS PHDDTKAFDT DLIAQWGPVD QYVGGVTHAI LHLLYARFIT KVLHDLGYLP 

       610        620        630        640        650        660 
FDEPFTRLLN QGMVQMDGSA MSKSKGNIVR LSDQLEEHGV DAIRLTMAFA GPPEDDIDWA 

       670        680        690        700        710        720 
DVSPSGSAKF LGRAWRLARD VTSEPGVDPT TGDAALRSVT HRTLHEVSQH VEAFRFNVAV 

       730        740        750        760        770        780 
ARLMELVNAT RKAIDSGPGA GDPAVREAVE ATAVVLSLFA PYAAEDMWSL LGHDPCVARA 

       790        800        810        820        830        840 
GWPAVDESLL VQDTVTCVVQ VQGKVRDRLE VSPDITQDAL RELALASEKV QAALGGRDLR 

       850 
TVVVRAPKLV NIVPA 

« Hide

References

[1]"Complete sequence of chromosome of Kineococcus radiotolerans SRS30216."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Lykidis A., Bagwell C.E., Shimkets L., Berry C.J., Fliermans C., Richardson P.
Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-149 / DSM 14245 / SRS30216.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000750 Genomic DNA. Translation: ABS04906.1.
RefSeqYP_001363170.1. NC_009664.2.

3D structure databases

ProteinModelPortalA6WDL7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING266940.Krad_3443.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABS04906; ABS04906; Krad_3443.
GeneID5335432.
KEGGkra:Krad_3443.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMALHMGHAE.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycKRAD266940:GI4N-3344-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_KINRD
AccessionPrimary (citable) accession number: A6WDL7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: August 21, 2007
Last modified: April 16, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries