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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Kineococcus radiotolerans (strain ATCC BAA-149 / DSM 14245 / SRS30216)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei623 – 6231ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciKRAD266940:GI4N-3344-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Krad_3443
OrganismiKineococcus radiotolerans (strain ATCC BAA-149 / DSM 14245 / SRS30216)
Taxonomic identifieri266940 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesKineosporiineaeKineosporiaceaeKineococcus
ProteomesiUP000001116 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 855855Leucine--tRNA ligasePRO_0000334765Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi266940.Krad_3443.

Structurei

3D structure databases

ProteinModelPortaliA6WDL7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi71 – 8111"HIGH" regionAdd
BLAST
Motifi620 – 6245"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiLHMGHAE.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6WDL7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRDARTHRED EMTQTDTQAT GPDRAEEQDG QRYDAFALQE KWLPVWDERK
60 70 80 90 100
PFRSGEPGDE RPKKYVLDMF PYPSGDLHMG HAEAYALGDV IARYWLQRGF
110 120 130 140 150
DVMHPIGWDA FGLPAENAAI KRGLDPRRWT YDNIAQQRAS MRRYACSFDW
160 170 180 190 200
DRVLHTSDPE YYRWNQWLFL KLYEKGLAYR KDSLVNWDPV DQTVLANEQV
210 220 230 240 250
LPDGTSERSG AKVVKKKLTQ WYFRITEYAD RLLDDLDTLE GQWPAKVISM
260 270 280 290 300
QRNWIGRSTG AEVEFAIEGR DEPVTVYTTR PDTLHGATFM VVAADSDLAE
310 320 330 340 350
ELAAGAPDDV RAAFEAYRTQ VQETSDIDRL SSEREKTGVP LGRYAVNPVN
360 370 380 390 400
GERLPIWAAD YVLADYGTGA IMAVPAHDQR DLDFARAFGL PVRVVVDVRD
410 420 430 440 450
DDGNPLPDPA ESGTATAGDG VLVNSGALDG LGKQAGIAKA IEDLTAAGKG
460 470 480 490 500
RAAKNYRLRD WLISRQRYWG TPIPVVHTEN GEVPVPEDQL PVLLPPSEGL
510 520 530 540 550
NLTPKGTSPL GAAEDWVNVP SPVDGSPARR DPDTMDTFVD SSWYFLRFVS
560 570 580 590 600
PHDDTKAFDT DLIAQWGPVD QYVGGVTHAI LHLLYARFIT KVLHDLGYLP
610 620 630 640 650
FDEPFTRLLN QGMVQMDGSA MSKSKGNIVR LSDQLEEHGV DAIRLTMAFA
660 670 680 690 700
GPPEDDIDWA DVSPSGSAKF LGRAWRLARD VTSEPGVDPT TGDAALRSVT
710 720 730 740 750
HRTLHEVSQH VEAFRFNVAV ARLMELVNAT RKAIDSGPGA GDPAVREAVE
760 770 780 790 800
ATAVVLSLFA PYAAEDMWSL LGHDPCVARA GWPAVDESLL VQDTVTCVVQ
810 820 830 840 850
VQGKVRDRLE VSPDITQDAL RELALASEKV QAALGGRDLR TVVVRAPKLV

NIVPA
Length:855
Mass (Da):94,721
Last modified:August 21, 2007 - v1
Checksum:i1FC3E953BCA4D622
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000750 Genomic DNA. Translation: ABS04906.1.
RefSeqiYP_001363170.1. NC_009664.2.

Genome annotation databases

EnsemblBacteriaiABS04906; ABS04906; Krad_3443.
KEGGikra:Krad_3443.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000750 Genomic DNA. Translation: ABS04906.1.
RefSeqiYP_001363170.1. NC_009664.2.

3D structure databases

ProteinModelPortaliA6WDL7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi266940.Krad_3443.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABS04906; ABS04906; Krad_3443.
KEGGikra:Krad_3443.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiLHMGHAE.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciKRAD266940:GI4N-3344-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-149 / DSM 14245 / SRS30216.

Entry informationi

Entry nameiSYL_KINRD
AccessioniPrimary (citable) accession number: A6WDL7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: August 21, 2007
Last modified: April 1, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.