A6WCF7 (SYA_KINRD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Krad_3032

Organism

Kineococcus radiotolerans (strain ATCC BAA-149 / DSM 14245 / SRS30216) [Complete proteome] [HAMAP]

Taxonomic identifier

266940 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Kineosporiineae › Kineosporiaceae › Kineococcus

Protein attributes

Sequence length	893 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_B
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_B
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00036_B.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_B
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 893	893	Alanine--tRNA ligase HAMAP MF_00036_B		PRO_0000347640
Sites
	Metal binding	573	1	Zinc Potential
	Metal binding	577	1	Zinc Potential
	Metal binding	676	1	Zinc Potential
	Metal binding	680	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	A6WCF7 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: EBF344A32DA0DB91	FASTA	893	95,953
	10 20 30 40 50 60 MQTAEIRRRW LDFFERKEHT VVPSASLVSS DPSLMFTVAG MVPFIPYLTA QVPAPYKRAT 70 80 90 100 110 120 SVQKCLRTLD IDEVGKTTRH GTFFQMNGNF SFGDYFKREA VAFAWELLTT PEADGGLGFD 130 140 150 160 170 180 PERLWTTVYL DDDEAFQLWR EVGMPAERIQ RRGKADNYWN TGQPGPGGPC SEIYFDRGPA 190 200 210 220 230 240 YGAEGGPEAD EDRYIEIWNL VFMQYQLSAV RTKVDFDVEG ELPAKNIDTG MGLERVAFLK 250 260 270 280 290 300 QGVDNMYEID EVRPVLDKAA ELSGRRYGAV HEDDVRMRVV ADHVRSALML IGDGVTPGNE 310 320 330 340 350 360 GAGYVLRRLV RRAVRAMRLL GVEEPALPHL LPASMEVMSA SYPQLRSDFE RISAVAYAEE 370 380 390 400 410 420 DAFRRTLVSG TAIFETAVAQ TKAAGGSSLS GERAFALHDT YGFPIDLTLE MAAEAGVGVD 430 440 450 460 470 480 EPGFRALMAE QVGRAKADAK AKKTGGVDLA IYRSTLEQLP APVVFTGYEA AAGEARISVV 490 500 510 520 530 540 LQGGVSTPSA PAGTDVEVVL DRTPFYAEGG GQLADHGTVT TTSGAVLAVS DVQQPVRGLY 550 560 570 580 590 600 VHKGTVTSGE LVAGDAVHAV VDAGRRRSIS RAHTATHLVH QVVREHLGDT ATQAGSQNAP 610 620 630 640 650 660 GRMRFDYRST AQVAGDVVRD IEAVVNERIH DDLEVSAAVM DRESALNSGA MALFGEKYGE 670 680 690 700 710 720 KVRVVSIGED WSKELCGGTH TLTSQQVGLV SIVSESSIGS GARRIEALVG ADAFDFLTRE 730 740 750 760 770 780 HLLVNQLTEV VKARPEELPD RIGALLTRLG DAEKEIARLR GGQVLALAPT IAAKPVDKFG 790 800 810 820 830 840 VRVVTHDAGP VSADDLRTLV LDVRSRLGEE RPSVVAVAGV AKDRPVVVVA TNAEARRWGV 850 860 870 880 890 KAGELVRTAA KTLGGGGGGK DDLAQGGGQD PSKVPAALQG IEDFVGARVT GSV

References

[1]

"Complete sequence of chromosome of Kineococcus radiotolerans SRS30216." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Lykidis A., Bagwell C.E., Shimkets L., Berry C.J., Fliermans C., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-149 / DSM 14245 / SRS30216.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000750 Genomic DNA. Translation: ABS04496.1.
RefSeq	YP_001362760.1. NC_009664.2.
3D structure databases
ProteinModelPortal	A6WCF7.
ModBase	Search...
Protein-protein interaction databases
STRING	A6WCF7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5336979.
GenomeReviews	Gene locus Krad_3032 in contig CP000750_GR.
KEGG	kra:Krad_3032.
NMPDR	fig|266940.1.peg.3403.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0013.
HOGENOM	HBG354397.
OMA	GESKTDQ.
ProtClustDB	PRK00252.
Enzyme and pathway databases
BioCyc	KRAD266940:KRAD_3032-MONOMER.
Family and domain databases
HAMAP	MF_00036_B. Ala_tRNA_synth_B. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR023033. Ala_tRNA_synth_euk/bac. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
PANTHER	PTHR11777:SF6. PTHR11777:SF6. 1 hit.
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00344. AlaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_KINRD

Accession

Primary (citable) accession number: A6WCF7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	August 21, 2007
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program