A6WCA2 (RISB_KINRD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6,7-dimethyl-8-ribityllumazine synthase
Short name=DMRL synthase
Short name=Lumazine synthase
EC=2.5.1.9

Alternative name(s):
Riboflavin synthase beta chain

Gene names

Name:	ribH
Ordered Locus Names:	Krad_2977

Organism

Kineococcus radiotolerans (strain ATCC BAA-149 / DSM 14245 / SRS30216) [Complete proteome] [HAMAP]

Taxonomic identifier

266940 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Kineosporiineae › Kineosporiaceae › Kineococcus

Protein attributes

Sequence length	161 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2-butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes the condensation of 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione with L-3,4-dihydrohy-2-butanone-4-phosphate yielding 6,7-dimethyl-8-lumazine By similarity. HAMAP MF_00178
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine. HAMAP MF_00178
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2. HAMAP MF_00178
Sequence similarities	Belongs to the DMRL synthase family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	riboflavin synthase complex Inferred from electronic annotation. Source: InterPro
Molecular function	riboflavin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 161	161	6,7-dimethyl-8-ribityllumazine synthase HAMAP MF_00178		PRO_1000077237

Sequences

	Sequence		Length	Mass (Da)
	A6WCA2 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 87C46992F241A673	FASTA	161	16,325
	10 20 30 40 50 60 MSGDGSPEIE VDGSGLRVAV VAARWHAETM DGLLAGARRA LEASGVTEVT EVRVPGAFEL 70 80 90 100 110 120 PVAAARLARA GHDAVVALGV VIRGGTPHFD YVCDAATQGL TQVAVTTGVP IGFGVLTVDN 130 140 150 160 EAQALHRAGL RGSREDKGAE AVLAALETVV ALRGVAPLPQ G

References

[1]

"Complete sequence of chromosome of Kineococcus radiotolerans SRS30216." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Lykidis A., Bagwell C.E., Shimkets L., Berry C.J., Fliermans C., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-149 / DSM 14245 / SRS30216.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000750 Genomic DNA. Translation: ABS04441.1.
RefSeq	YP_001362705.1. NC_009664.2.
3D structure databases
ProteinModelPortal	A6WCA2.
SMR	A6WCA2. Positions 11-138.
ModBase	Search...
Protein-protein interaction databases
STRING	A6WCA2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5337640.
GenomeReviews	Gene locus Krad_2977 in contig CP000750_GR.
KEGG	kra:Krad_2977.
NMPDR	fig|266940.1.peg.2276.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0054.
HOGENOM	HBG311126.
OMA	SSRALMD.
Enzyme and pathway databases
BioCyc	KRAD266940:KRAD_2977-MONOMER.
Family and domain databases
HAMAP	MF_00178. Lumazine_synth. [Tree]
InterPro	IPR002180. DMRL_synthase. [Graphical view]
Gene3D	G3DSA:3.40.50.960. DMRL_synthase. 1 hit.
KO	K00794.
PANTHER	PTHR21058. DMRL_synthase. 1 hit.
Pfam	PF00885. DMRL_synthase. 1 hit. [Graphical view]
SUPFAM	SSF52121. DMRL_synthase. 1 hit.
TIGRFAMs	TIGR00114. Lumazine-synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RISB_KINRD

Accession

Primary (citable) accession number: A6WCA2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	August 21, 2007
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program