A6W9A6 (A6W9A6_KINRD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase 2 HAMAP MF_00012
Short name=DAD 2 HAMAP MF_00012
EC=4.2.1.9 HAMAP MF_00012

Gene names

Name:	ilvD2 HAMAP MF_00012
Ordered Locus Names:	Krad_1909

Organism

Kineococcus radiotolerans (strain ATCC BAA-149 / DSM 14245 / SRS30216) [Complete proteome] [HAMAP] EMBL ABS03395.1

Taxonomic identifier

266940 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Kineosporiineae › Kineosporiaceae › Kineococcus

Protein attributes

Sequence length	619 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster By similarity. HAMAP MF_00012
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family. HAMAP MF_00012

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis HAMAP MF_00012
Ligand	4Fe-4S HAMAP MF_00012 Iron Iron-sulfur Metal-binding
Molecular function	Lyase HAMAP MF_00012 EMBL ABS03395.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) By similarity HAMAP MF_00012
	Metal binding	197	1	Iron-sulfur (4Fe-4S) By similarity HAMAP MF_00012

Sequences

	Sequence		Length	Mass (Da)
	A6W9A6 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 61109126B712ED6E	FASTA	619	65,045
	10 20 30 40 50 60 MPALRSRTST HGRNMAGARA LWRATGMTDG DFGKPIVAIA NSYTQFVPGH VHLKDMGDLV 70 80 90 100 110 120 AGAIAEAGGV SKEFNTIAVD DGIAMGHSGM LYSLPSRELI ADSVEYMVNA HQADALVCIS 130 140 150 160 170 180 NCDKITPGML MAALRLNIPV VFVSGGPMEA GKAVVVDGVA HAPTDLVTAI SASASEAVDA 190 200 210 220 230 240 EGLAQVERSA CPTCGSCSGM FTANSMNCLT EALGLSLPGN GSTLATHAAR RDLFLRAGTL 250 260 270 280 290 300 IVELAKQYYD GDDESVLPRS IANRAAFSNA MALDVAMGGS TNTVLHILAA AQEAELDFGL 310 320 330 340 350 360 DDIDAISRRV PCLSKVAPNS DFHMEDVHRA GGIPAILGEL WRGGMLDDSV RTVHSPDVAS 370 380 390 400 410 420 WLERWDVRAE SPSEVALELF HAAPGGVRTT QAFSTENRWS SLDTDAATGC VRDVAHAYTA 430 440 450 460 470 480 DGGLAVLEGN LATEGAIIKT AGIDPSLFHF RGTALVLESQ EEAVEAILSG KVRPGHVVVI 490 500 510 520 530 540 RYEGPAGGPG MQEMLHPTSF LKGRGLGKVC ALVTDGRFSG GSSGISVGHV SPEAWAGGVI 550 560 570 580 590 600 GLVEDGDEIE IDVESRGLRL LVDDEVLAER RAKMESSERP WEPRDRVRPL TGALRAYAAM 610 ATSASTGAVR RVPERRGRS

References

[1]

"Complete sequence of chromosome of Kineococcus radiotolerans SRS30216." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Lykidis A., Bagwell C.E., Shimkets L., Berry C.J., Fliermans C., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-149 / DSM 14245 / SRS30216.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000750 Genomic DNA. Translation: ABS03395.1.
RefSeq	YP_001361659.1. NC_009664.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6W9A6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5337352.
GenomeReviews	Gene locus Krad_1909 in contig CP000750_GR.
KEGG	kra:Krad_1909.
NMPDR	fig|266940.1.peg.4541.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0129.
HOGENOM	HBG671001.
OMA	MACVTET.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A6W9A6_KINRD

Accession

Primary (citable) accession number: A6W9A6

Entry history

Integrated into UniProtKB/TrEMBL:	August 21, 2007
Last sequence update:	August 21, 2007
Last modified:	December 14, 2011
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)