A6W961 (T23O_KINRD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan 2,3-dioxygenase
Short name=TDO
EC=1.13.11.11

Alternative name(s):
Tryptamin 2,3-dioxygenase
Tryptophan oxygenase
Short name=TO
Short name=TRPO
Tryptophan pyrrolase
Tryptophanase

Gene names

Name:	kynA
Ordered Locus Names:	Krad_1864

Organism

Kineococcus radiotolerans (strain ATCC BAA-149 / DSM 14245 / SRS30216) [Complete proteome] [HAMAP]

Taxonomic identifier

266940 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Kineosporiineae › Kineosporiaceae › Kineococcus

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB tryptophan catabolic process to kynurenine Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	heme binding Inferred from sequence or structural similarity. Source: UniProtKB tryptophan 2,3-dioxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	Tryptophan 2,3-dioxygenase		PRO_0000360118
Regions
	Region	26 – 30	5	Substrate binding By similarity
	Region	53 – 57	5	Substrate binding By similarity
Sites
	Metal binding	242	1	Iron (heme axial ligand) By similarity
	Binding site	115	1	Substrate By similarity
	Binding site	119	1	Substrate By similarity
	Binding site	126	1	Heme By similarity
	Binding site	256	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6W961 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 994E31D1A084C133	FASTA	286	32,598
	10 20 30 40 50 60 MSGTAEENTR EVEAGVVTDF TREMSYGEYL HLDELLAAQH PLSTPEHHDE LLFIVQHQTS 70 80 90 100 110 120 ELWLKLVLHE LRSAMRAIAA DDLKTALKNI ARVKHIQRTL TEQWSVLATL TPTEYAQFRG 130 140 150 160 170 180 FLANSSGFQS QQYRAVEFAL GNKNAKMLDV FAHDGPGHAQ LTELLEAPSL YDEFLRHLAR 190 200 210 220 230 240 RGHDVPAELL ERDVTKAHVH TPALVATFRV IYEDAQRYWT EYEACEELVD LEENFQLWRF 250 260 270 280 RHLKTVERTI GFKRGTGGSS GVGFLARALD LTFFPELYAV RTEIGS

References

[1]

"Complete sequence of chromosome of Kineococcus radiotolerans SRS30216." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Lykidis A., Bagwell C.E., Shimkets L., Berry C.J., Fliermans C., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-149 / DSM 14245 / SRS30216.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000750 Genomic DNA. Translation: ABS03350.1.
RefSeq	YP_001361614.1. NC_009664.2.
3D structure databases
ProteinModelPortal	A6W961.
SMR	A6W961. Positions 24-285.
ModBase	Search...
Protein-protein interaction databases
STRING	A6W961.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5338030.
GenomeReviews	Gene locus Krad_1864 in contig CP000750_GR.
KEGG	kra:Krad_1864.
NMPDR	fig|266940.1.peg.1728.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3483.
HOGENOM	HBG647485.
OMA	QYREIEF.
ProtClustDB	CLSK2792490.
Enzyme and pathway databases
BioCyc	KRAD266940:KRAD_1864-MONOMER.
Family and domain databases
InterPro	IPR017485. Trp_2-3-dOase_bac. IPR004981. Trp_2_3_dOase. [Graphical view]
KO	K00453.
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 2 hits. [Graphical view]
TIGRFAMs	TIGR03036. Trp_2_3_diox. 1 hit.
ProtoNet	Search...

Entry information

Entry name

T23O_KINRD

Accession

Primary (citable) accession number: A6W961

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	August 21, 2007
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program