A6W8A4 (A6W8A4_KINRD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP MF_00116
Short name=dUTPase HAMAP MF_00116
EC=3.6.1.23 HAMAP MF_00116

Alternative name(s):
dUTP pyrophosphatase HAMAP MF_00116

Gene names

Name:	dut HAMAP MF_00116
Ordered Locus Names:	Krad_1557

Organism

Kineococcus radiotolerans (strain ATCC BAA-149 / DSM 14245 / SRS30216) [Complete proteome] [HAMAP] EMBL ABS03043.1

Taxonomic identifier

266940 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Kineosporiineae › Kineosporiaceae › Kineococcus

Protein attributes

Sequence length	181 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity. HAMAP MF_00116
Catalytic activity	dUTP + H2O = dUMP + diphosphate. HAMAP MF_00116
Cofactor	Magnesium By similarity. HAMAP MF_00116
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP MF_00116
Sequence similarities	Belongs to the dUTPase family. HAMAP MF_00116

Ontologies

Keywords
Biological process	Nucleotide metabolism HAMAP MF_00116
Ligand	Magnesium HAMAP MF_00116 Metal-binding HAMAP MF_00116
Molecular function	Hydrolase HAMAP MF_00116 EMBL ABS03043.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	dUTP diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	73 – 75	3	Substrate binding By similarity HAMAP MF_00116
	Region	90 – 92	3	Substrate binding By similarity HAMAP MF_00116
Sites
	Binding site	86	1	Substrate By similarity HAMAP MF_00116

Sequences

	Sequence		Length	Mass (Da)
	A6W8A4 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 18CD97184BB09DD9	FASTA	181	18,700
	10 20 30 40 50 60 MSSPVTDQLQ PTLAVQTVLT VPEALPTYAH PGDAGADLTT RVDVVVGPGE RVNVPTGVSI 70 80 90 100 110 120 ALPAGYAAFV VPRSGLAAKR GLTTLNAPGT VDAGYRGEIR VTLHNTDLRE AVELRAGDRI 130 140 150 160 170 180 AQLVVQRVER VEFVPVPQLP GSARGAGGHG STGGSEALHP HRPTVGTTPS TTPSVTPEER P

References

[1]

"Complete sequence of chromosome of Kineococcus radiotolerans SRS30216." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Lykidis A., Bagwell C.E., Shimkets L., Berry C.J., Fliermans C., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-149 / DSM 14245 / SRS30216.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000750 Genomic DNA. Translation: ABS03043.1.
RefSeq	YP_001361307.1. NC_009664.2.
3D structure databases
ProteinModelPortal	A6W8A4.
SMR	A6W8A4. Positions 24-139.
ModBase	Search...
Protein-protein interaction databases
STRING	A6W8A4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5337883.
GenomeReviews	Gene locus Krad_1557 in contig CP000750_GR.
KEGG	kra:Krad_1557.
NMPDR	fig|266940.1.peg.2858.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0756.
HOGENOM	HBG436079.
OMA	KIAQMVI.
Family and domain databases
HAMAP	MF_00116. dUTPase_bact. [Tree]
InterPro	IPR008180. dUTP_pyroPase. IPR008181. dUTP_pyroPase_sf. [Graphical view]
KO	K01520.
PANTHER	PTHR11241. PTHR11241. 1 hit.
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00576. Dut. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A6W8A4_KINRD

Accession

Primary (citable) accession number: A6W8A4

Entry history

Integrated into UniProtKB/TrEMBL:	August 21, 2007
Last sequence update:	August 21, 2007
Last modified:	November 16, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)