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Protein

tRNA dimethylallyltransferase

Gene

miaA

Organism
Kineococcus radiotolerans (strain ATCC BAA-149 / DSM 14245 / SRS30216)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i6A).UniRule annotation

Catalytic activityi

Dimethylallyl diphosphate + adenine(37) in tRNA = diphosphate + N(6)-dimethylallyladenine(37) in tRNA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 20ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA dimethylallyltransferaseUniRule annotation (EC:2.5.1.75UniRule annotation)
Alternative name(s):
Dimethylallyl diphosphate:tRNA dimethylallyltransferaseUniRule annotation
Short name:
DMAPP:tRNA dimethylallyltransferaseUniRule annotation
Short name:
DMATaseUniRule annotation
Isopentenyl-diphosphate:tRNA isopentenyltransferaseUniRule annotation
Short name:
IPP transferaseUniRule annotation
Short name:
IPPTUniRule annotation
Short name:
IPTaseUniRule annotation
Gene namesi
Name:miaAUniRule annotation
Ordered Locus Names:Krad_1500
OrganismiKineococcus radiotolerans (strain ATCC BAA-149 / DSM 14245 / SRS30216)
Taxonomic identifieri266940 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaKineosporialesKineosporiaceaeKineococcus
Proteomesi
  • UP000001116 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003771941 – 317tRNA dimethylallyltransferaseAdd BLAST317

Interactioni

Subunit structurei

Monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei110Interaction with substrate tRNAUniRule annotation1
Sitei131Interaction with substrate tRNAUniRule annotation1

Protein-protein interaction databases

STRINGi266940.Krad_1500.

Structurei

3D structure databases

ProteinModelPortaliA6W849.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni15 – 20Substrate bindingUniRule annotation6

Sequence similaritiesi

Belongs to the IPP transferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DKX. Bacteria.
COG0324. LUCA.
HOGENOMiHOG000039995.
KOiK00791.
OMAiHTRNYAK.
OrthoDBiPOG091H00NK.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00185. IPP_trans. 1 hit.
InterProiIPR018022. IPP_trans.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01715. IPPT. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00174. miaA. 1 hit.

Sequencei

Sequence statusi: Complete.

A6W849-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQPNVPLVA VVGPTASGKS DVGVALAHLL EREHGRPGEV VNADAMQLYR
60 70 80 90 100
GMDVGTAKLT PAEREGVPHH LLDVLDVTET AEVARFQADA RAAVEDVTAR
110 120 130 140 150
GGLPLLVGGS GLYVRAAVDD LRFPGTDPEV RARWEAELAV LGPHALHARL
160 170 180 190 200
AERDPAAAAK ILPGNGRRIV RALEVGELTG RPFAASLPEQ TYLRPTVQVG
210 220 230 240 250
LAVPREQLDA RIDARVERMW AAGLVAEVRD LEARGLREGR TASRALGYAQ
260 270 280 290 300
VLDAFDGTTT EDEARELTAR LTRRFARKQE SWFRRDPRVH WLPAPDGSDP
310
LDLARRVLEL LPVASAA
Length:317
Mass (Da):34,276
Last modified:August 21, 2007 - v1
Checksum:i7C3241444231ADED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000750 Genomic DNA. Translation: ABS02988.1.
RefSeqiWP_011981873.1. NC_009664.2.

Genome annotation databases

EnsemblBacteriaiABS02988; ABS02988; Krad_1500.
KEGGikra:Krad_1500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000750 Genomic DNA. Translation: ABS02988.1.
RefSeqiWP_011981873.1. NC_009664.2.

3D structure databases

ProteinModelPortaliA6W849.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi266940.Krad_1500.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABS02988; ABS02988; Krad_1500.
KEGGikra:Krad_1500.

Phylogenomic databases

eggNOGiENOG4105DKX. Bacteria.
COG0324. LUCA.
HOGENOMiHOG000039995.
KOiK00791.
OMAiHTRNYAK.
OrthoDBiPOG091H00NK.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00185. IPP_trans. 1 hit.
InterProiIPR018022. IPP_trans.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01715. IPPT. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00174. miaA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMIAA_KINRD
AccessioniPrimary (citable) accession number: A6W849
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: August 21, 2007
Last modified: November 2, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.