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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Kineococcus radiotolerans (strain ATCC BAA-149 / DSM 14245 / SRS30216)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi520 – 5201MagnesiumUniRule annotation
Metal bindingi526 – 5261MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciKRAD266940:GI4N-759-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:Krad_1466
OrganismiKineococcus radiotolerans (strain ATCC BAA-149 / DSM 14245 / SRS30216)
Taxonomic identifieri266940 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesKineosporiineaeKineosporiaceaeKineococcus
ProteomesiUP000001116 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 746746Polyribonucleotide nucleotidyltransferasePRO_0000329686Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi266940.Krad_1466.

Structurei

3D structure databases

ProteinModelPortaliA6W815.
SMRiA6W815. Positions 5-723.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini586 – 64863KHUniRule annotationAdd
BLAST
Domaini657 – 72973S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR014069. pppGpp_PNP.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
TIGR02696. pppGpp_PNP. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6W815-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGPEITAAE AVIDNGSFGT RTIRFETGRL AKQAAGSAAV YLDGESFLLS
60 70 80 90 100
ATTAGKSPKD QFDFFPLTVD VEERSYAAGK IPGSFFRREG RPSTEAILTC
110 120 130 140 150
RLIDRPLRPS FVKGLRNEVQ VVVSVMALHP DDAYDVVAIN VASLSTQLSG
160 170 180 190 200
LPFSGPIGGV RIALIDGQWV AFPRYSELER AVFDMVVAGR VVTTADGSQD
210 220 230 240 250
VAIMMVEAEA TEGSWNLIKD QGATAPTEEV VAAGLEAAKP FIAELVRAQA
260 270 280 290 300
EVAATAAKPT AAFPTFPDYQ DDVYQAVFEA ASADLTAALQ IGGKQDRETR
310 320 330 340 350
IDEVKEAVKA QLADAFAGRE KEVSAAYRSV QKALIRQRVL KDGVRIDGRG
360 370 380 390 400
LADIRTLSAE VEVLPRVHGS ALFERGETQI LGVTTLNMLR MEQQLDTLSP
410 420 430 440 450
VTRKRYMHNY NFPPYSTGET GRVGSPKRRE IGHGALAERA LVPVLPARED
460 470 480 490 500
FPYAIRQVSE ALGSNGSTSM GSVCASTLSL LNAGVPLRAA VAGIAMGLIS
510 520 530 540 550
DTVDGETRYA ALTDILGAED AFGDMDFKVA GTKEFVTAIQ LDTKLDGIPA
560 570 580 590 600
SVLAGALTQA RDARLHILDV MAEAIDAPDE MSPTAPRIIT VKVPVDKIGE
610 620 630 640 650
VIGPKGKMIN QIQEDTGADI SIEDDGTVFI GAVDGPSAEA ARAAVNAIAN
660 670 680 690 700
PTMPEVGERY LGTVVKTTTF GAFVSLMPGK DGLLHISQLR KLSGGKRVDN
710 720 730 740
VEDVVAVGQK VQVEIAEIDP RGKLSLVPVV AEEASAEAAA AEPADA
Length:746
Mass (Da):79,078
Last modified:August 21, 2007 - v1
Checksum:i0DAAFC8956295C99
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000750 Genomic DNA. Translation: ABS02954.1.
RefSeqiWP_011981907.1. NC_009664.2.
YP_001361218.1. NC_009664.2.

Genome annotation databases

EnsemblBacteriaiABS02954; ABS02954; Krad_1466.
KEGGikra:Krad_1466.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000750 Genomic DNA. Translation: ABS02954.1.
RefSeqiWP_011981907.1. NC_009664.2.
YP_001361218.1. NC_009664.2.

3D structure databases

ProteinModelPortaliA6W815.
SMRiA6W815. Positions 5-723.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi266940.Krad_1466.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABS02954; ABS02954; Krad_1466.
KEGGikra:Krad_1466.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Enzyme and pathway databases

BioCyciKRAD266940:GI4N-759-MONOMER.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR014069. pppGpp_PNP.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
TIGR02696. pppGpp_PNP. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-149 / DSM 14245 / SRS30216.

Entry informationi

Entry nameiPNP_KINRD
AccessioniPrimary (citable) accession number: A6W815
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: August 21, 2007
Last modified: May 27, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.