A6W7V4 (RNH2_KINRD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease HII
Short name=RNase HII
EC=3.1.26.4

Gene names

Name:	rnhB
Ordered Locus Names:	Krad_1405

Organism

Kineococcus radiotolerans (strain ATCC BAA-149 / DSM 14245 / SRS30216) [Complete proteome] [HAMAP]

Taxonomic identifier

266940 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Kineosporiineae › Kineosporiaceae › Kineococcus

Protein attributes

Sequence length	262 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that specifically degrades the RNA of RNA-DNA hybrids By similarity. HAMAP MF_00052_B
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP MF_00052_B
Cofactor	Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding By similarity.
Subcellular location	Cytoplasm Potential HAMAP MF_00052_B.
Sequence similarities	Belongs to the RNase HII family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease H activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 262	262	Ribonuclease HII HAMAP MF_00052_B		PRO_0000334909
Sites
	Metal binding	30	1	Divalent metal cation By similarity
	Metal binding	31	1	Divalent metal cation By similarity
	Metal binding	124	1	Divalent metal cation By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6W7V4 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 8B40BDE2AB19F6CC	FASTA	262	26,746
	10 20 30 40 50 60 MSPAGGRVPP SLRLERQFLR AGHALVAGVD EVGRGSLAGP VSVGVLVVDA ATRTAPTGLR 70 80 90 100 110 120 DSKLLTPAAR EALAPKVRRW AVASAVGHAE PGEIDAVGIV AALRLAGRRA LAQLPVPPDL 130 140 150 160 170 180 VILDGNHDWL SDPREPSLLD ALDGLGDPAA GLPCPAPAVT TRVKADVTCA AVAGASVLAK 190 200 210 220 230 240 TTRDALMTAR HEEFPHYGWA GNKGYSAPDH LEALAAHGAC PQHRRSWRLP GVAAAAGVPA 250 260 PRSGPGDALV DVTSDTSSPR GA

References

[1]

"Complete sequence of chromosome of Kineococcus radiotolerans SRS30216." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Lykidis A., Bagwell C.E., Shimkets L., Berry C.J., Fliermans C., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-149 / DSM 14245 / SRS30216.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000750 Genomic DNA. Translation: ABS02893.1.
RefSeq	YP_001361157.1. NC_009664.2.
3D structure databases
ProteinModelPortal	A6W7V4.
ModBase	Search...
Protein-protein interaction databases
STRING	A6W7V4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5336524.
GenomeReviews	Gene locus Krad_1405 in contig CP000750_GR.
KEGG	kra:Krad_1405.
NMPDR	fig|266940.1.peg.4376.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0164.
HOGENOM	HBG584843.
OMA	DGKHDYL.
Enzyme and pathway databases
BioCyc	KRAD266940:KRAD_1405-MONOMER.
Family and domain databases
HAMAP	MF_00052_B. RNase_HII_B. [Tree]
InterPro	IPR022898. RNase_HII. IPR001352. RNase_HII/HIII. IPR024567. RNase_HII/HIII_dom. IPR012337. RNaseH-like_dom. [Graphical view]
KO	K03470.
PANTHER	PTHR10954. RNase_HII/HIII. 1 hit.
Pfam	PF01351. RNase_HII. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RNH2_KINRD

Accession

Primary (citable) accession number: A6W7V4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	August 21, 2007
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program