A6W7Q5 (SYE_KINRD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	Krad_1356

Organism

Kineococcus radiotolerans (strain ATCC BAA-149 / DSM 14245 / SRS30216) [Complete proteome] [HAMAP]

Taxonomic identifier

266940 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Kineosporiineae › Kineosporiaceae › Kineococcus

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 501	501	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_0000330977
Regions
	Motif	21 – 31	11	"HIGH" region HAMAP MF_00022_B
	Motif	266 – 270	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	269	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6W7Q5 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 70EE9152537C7723	FASTA	501	54,661
	10 20 30 40 50 60 MTDIAAPPAP GTSPVRVRFC PSPTGTPHVG LIRTALFNWA HARHTGGTFV FRIEDTDAAR 70 80 90 100 110 120 DSEESYAQIL DALDWLGLDW DEGIGVGGPH EPYRQSLRRP VYDDVVRRLR EAGHLYESFS 130 140 150 160 170 180 TAEEIEARHE AAGRPKVLGY DGFDRDLTPE QVAAFRAEGR EPTLRLRLPE GRDYSFDDVV 190 200 210 220 230 240 RGRIEFKAGS FPDPVLVRAN GDPLYTLVNP VDDALMGITH VLRGEDLLSS TPRQIALHEA 250 260 270 280 290 300 LVDIGVSQAV PLFGHLPYVT GEGSKKLSKR DPESNLFHHR DRGFIREGLL NYLALLGWGI 310 320 330 340 350 360 SEDRDVFSVE EMVAAFDVAD VNASPARFDV AKAEAINAAH LRLLAPEDFR GRLVPYLQAA 370 380 390 400 410 420 GVLPAQPSPE QLERLAAAAP LVQERMTLLG QAPGMLGFLF VADGDVVLEP DATAALRPEA 430 440 450 460 470 480 ADVLDASLAA LEALTGWTTA DLEAALRAAV VEGLGVKPKF AFAPLRTAVT GRRVSPPLFE 490 500 SMEILGRESS LARLRRLRAT L

References

[1]

"Complete sequence of chromosome of Kineococcus radiotolerans SRS30216." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Lykidis A., Bagwell C.E., Shimkets L., Berry C.J., Fliermans C., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-149 / DSM 14245 / SRS30216.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000750 Genomic DNA. Translation: ABS02844.1.
RefSeq	YP_001361108.1. NC_009664.2.
3D structure databases
ProteinModelPortal	A6W7Q5.
ModBase	Search...
Protein-protein interaction databases
STRING	A6W7Q5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5335836.
GenomeReviews	Gene locus Krad_1356 in contig CP000750_GR.
KEGG	kra:Krad_1356.
NMPDR	fig|266940.1.peg.1365.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	ESIIQFV.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	KRAD266940:KRAD_1356-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_KINRD

Accession

Primary (citable) accession number: A6W7Q5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	August 21, 2007
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program