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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Kineococcus radiotolerans (strain ATCC BAA-149 / DSM 14245 / SRS30216)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei269ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:Krad_1356
OrganismiKineococcus radiotolerans (strain ATCC BAA-149 / DSM 14245 / SRS30216)
Taxonomic identifieri266940 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaKineosporialesKineosporiaceaeKineococcus
Proteomesi
  • UP000001116 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003309771 – 501Glutamate--tRNA ligaseAdd BLAST501

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi266940.Krad_1356

Structurei

3D structure databases

ProteinModelPortaliA6W7Q5
SMRiA6W7Q5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi21 – 31"HIGH" regionUniRule annotationAdd BLAST11
Motifi266 – 270"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252720
KOiK01885
OMAiHYINTLP
OrthoDBiPOG091H021W

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
1.10.1160.10, 1 hit
3.40.50.620, 2 hits
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR020061 Glu_tRNA_lig_a-bdl
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit

Sequencei

Sequence statusi: Complete.

A6W7Q5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDIAAPPAP GTSPVRVRFC PSPTGTPHVG LIRTALFNWA HARHTGGTFV
60 70 80 90 100
FRIEDTDAAR DSEESYAQIL DALDWLGLDW DEGIGVGGPH EPYRQSLRRP
110 120 130 140 150
VYDDVVRRLR EAGHLYESFS TAEEIEARHE AAGRPKVLGY DGFDRDLTPE
160 170 180 190 200
QVAAFRAEGR EPTLRLRLPE GRDYSFDDVV RGRIEFKAGS FPDPVLVRAN
210 220 230 240 250
GDPLYTLVNP VDDALMGITH VLRGEDLLSS TPRQIALHEA LVDIGVSQAV
260 270 280 290 300
PLFGHLPYVT GEGSKKLSKR DPESNLFHHR DRGFIREGLL NYLALLGWGI
310 320 330 340 350
SEDRDVFSVE EMVAAFDVAD VNASPARFDV AKAEAINAAH LRLLAPEDFR
360 370 380 390 400
GRLVPYLQAA GVLPAQPSPE QLERLAAAAP LVQERMTLLG QAPGMLGFLF
410 420 430 440 450
VADGDVVLEP DATAALRPEA ADVLDASLAA LEALTGWTTA DLEAALRAAV
460 470 480 490 500
VEGLGVKPKF AFAPLRTAVT GRRVSPPLFE SMEILGRESS LARLRRLRAT

L
Length:501
Mass (Da):54,661
Last modified:August 21, 2007 - v1
Checksum:i70EE9152537C7723
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000750 Genomic DNA Translation: ABS02844.1
RefSeqiWP_012084293.1, NC_009664.2

Genome annotation databases

EnsemblBacteriaiABS02844; ABS02844; Krad_1356
KEGGikra:Krad_1356

Similar proteinsi

Entry informationi

Entry nameiSYE_KINRD
AccessioniPrimary (citable) accession number: A6W7Q5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: August 21, 2007
Last modified: March 28, 2018
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health