A6W7J3 (A6W7J3_KINRD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB HAMAP MF_00685
EC=2.4.1.18 HAMAP MF_00685

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase HAMAP MF_00685
Alpha-(1->4)-glucan branching enzyme HAMAP MF_00685
Glycogen branching enzyme HAMAP MF_00685

Gene names

Name:	glgB HAMAP MF_00685
Ordered Locus Names:	Krad_1294

Organism

Kineococcus radiotolerans (strain ATCC BAA-149 / DSM 14245 / SRS30216) [Complete proteome] [HAMAP] EMBL ABS02782.1

Taxonomic identifier

266940 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Kineosporiineae › Kineosporiaceae › Kineococcus

Protein attributes

Sequence length	732 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685 SAAS SAAS006407
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685 SAAS SAAS006407
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily. HAMAP MF_00685

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis HAMAP MF_00685 SAAS SAAS006407 Glycogen metabolism HAMAP MF_00685
Molecular function	Glycosyltransferase HAMAP MF_00685 SAAS SAAS006407 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	413	1	Nucleophile By similarity HAMAP MF_00685
	Active site	466	1	Proton donor By similarity HAMAP MF_00685

Sequences

	Sequence		Length	Mass (Da)
	A6W7J3 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: AB181AC91038DF2B	FASTA	732	81,165
	10 20 30 40 50 60 MSSEAPSSAP TPRPVDADDL ARAAAGAHWS PHSVLGVHPH GDALTYRVLK PFASAVTVVT 70 80 90 100 110 120 EDGTRVGLVH EYDGVWVGVL PGAGVGDYRV EVTYPGAEPA LLDDPYRFLP TVGEVDLHLI 130 140 150 160 170 180 GEGRHEELWN VLGAHVRHWE GPMGPVEGTS FAVWAPAARA VRVVGDHNHW SGREHAMRSL 190 200 210 220 230 240 GSSGVWEIFL PGVGVGSRYK YEILGADGHW RQKADPMARA SEVPPATASV VTESTYTWND 250 260 270 280 290 300 AEWLEQRAAR SAQDGPVSVY EVHLGSWKQG LSYTELATEL VDYVSAAGFT HVEFLPVMEH 310 320 330 340 350 360 PFGGSWGYQV TGYFAPTSRF GSPDELRHLI DALHQAGIGV IIDWVPGHFP KDEFALAKFD 370 380 390 400 410 420 GTALYEHPDP RLGEHMDWGT YIPNFGRSEV RNFLVASGLY WLEEFHADGL RVDAVASMLY 430 440 450 460 470 480 LDYSRNAGEW VPNRHGGREN LEAIAFLQEA NATAYKRTPG IMMIAEESTA FPGVTAPTSA 490 500 510 520 530 540 GGLGFGLKWN MGWMNDSLRY FAQDPVNRQY HHGELTFSLV YAFTENFALP ISHDEVVHGK 550 560 570 580 590 600 GSLLRKMPGD RWQQLANVRA FLGYMWSHPG KQLLFMGSEF GQEAEWSESR SLDWWLLDTP 610 620 630 640 650 660 WHQGLHRLVT RLNAVYKEHP ELWSDDFTSE GFQWIDGADN QRNLISFVRW SKDRKPLVAV 670 680 690 700 710 720 CNFSGSPYED FRVGLPQAGP WREVLNTDAE EFAGSGVVNT GTLQGEDVPW NGQQFSAALR 730 VPPLGVTWLV PA

References

[1]

"Complete sequence of chromosome of Kineococcus radiotolerans SRS30216." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Lykidis A., Bagwell C.E., Shimkets L., Berry C.J., Fliermans C., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-149 / DSM 14245 / SRS30216.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000750 Genomic DNA. Translation: ABS02782.1.
RefSeq	YP_001361046.1. NC_009664.2.
3D structure databases
ProteinModelPortal	A6W7J3.
ModBase	Search...
Protein-protein interaction databases
STRING	A6W7J3.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5334648.
GenomeReviews	Gene locus Krad_1294 in contig CP000750_GR.
KEGG	kra:Krad_1294.
NMPDR	fig|266940.1.peg.1219.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0296.
HOGENOM	HBG287139.
OMA	DGTCLYE.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
KO	K00700.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR01515. Branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A6W7J3_KINRD

Accession

Primary (citable) accession number: A6W7J3

Entry history

Integrated into UniProtKB/TrEMBL:	August 21, 2007
Last sequence update:	August 21, 2007
Last modified:	November 16, 2011
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)