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Protein

D-inositol 3-phosphate glycosyltransferase

Gene

mshA

Organism
Kineococcus radiotolerans (strain ATCC BAA-149 / DSM 14245 / SRS30216)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2-acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway.UniRule annotation

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate = UDP + 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei16 – 1611D-inositol 3-phosphateUniRule annotation
Binding sitei30 – 301UDP-GlcNAc; via amide nitrogenUniRule annotation
Binding sitei85 – 8511D-inositol 3-phosphateUniRule annotation
Binding sitei118 – 11811D-inositol 3-phosphateUniRule annotation
Binding sitei142 – 14211D-inositol 3-phosphateUniRule annotation
Binding sitei162 – 16211D-inositol 3-phosphateUniRule annotation
Binding sitei237 – 2371UDP-GlcNAcUniRule annotation
Binding sitei242 – 2421UDP-GlcNAcUniRule annotation
Binding sitei303 – 3031UDP-GlcNAc; via amide nitrogen and carbonyl oxygenUniRule annotation
Metal bindingi312 – 3121Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi313 – 3131Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi315 – 3151Magnesium; via carbonyl oxygenUniRule annotation
Binding sitei325 – 3251UDP-GlcNAcUniRule annotation
Binding sitei333 – 3331UDP-GlcNAcUniRule annotation
Metal bindingi339 – 3391MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciKRAD266940:GI4N-1344-MONOMER.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
D-inositol 3-phosphate glycosyltransferase (EC:2.4.1.250UniRule annotation)
Alternative name(s):
N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferaseUniRule annotation
Short name:
GlcNAc-Ins-P N-acetylglucosaminyltransferaseUniRule annotation
Gene namesi
Name:mshAUniRule annotation
Ordered Locus Names:Krad_0890
OrganismiKineococcus radiotolerans (strain ATCC BAA-149 / DSM 14245 / SRS30216)
Taxonomic identifieri266940 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaKineosporialesKineosporiaceaeKineococcus
Proteomesi
  • UP000001116 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 435435D-inositol 3-phosphate glycosyltransferasePRO_0000400128Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi266940.Krad_0890.

Structurei

3D structure databases

ProteinModelPortaliA6W6D9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni22 – 232UDP-GlcNAc bindingUniRule annotation
Regioni27 – 3261D-inositol 3-phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyltransferase group 1 family. MshA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiPOG091H0AO3.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.

Sequencei

Sequence statusi: Complete.

A6W6D9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRSSSVQRV ALLSVHTSPL AQPGTGDAGG MNVYVVELAT QLARRGVEVE
60 70 80 90 100
VFTRRTSSEQ PPVVETADGV RVRHVAAGPY EGLAKDDLPG QLCAFTAGML
110 120 130 140 150
HAEARHAERH YDLVHSHYWL SGQVGWLTAD RWDVPLVHSM HTMAKVKNAA
160 170 180 190 200
LAEGDAPEPA GRVIGEQQVV EAADRLVANT DAERRELIDL YGADPAKVVV
210 220 230 240 250
VPPGVDLATF APAPGRAASR ARLGVPADAE VLLFVGRIQP LKAPDLLVRA
260 270 280 290 300
TAELLREQPW RRSRLRVVVL GGPSGSGTAH PDSLADLVRS LDLEDVVRMA
310 320 330 340 350
PPVARAELAD HYRAADVVAV PSHNESFGLV ALEAQACATP VVAAAVGGLR
360 370 380 390 400
TAVLDDGAGA GTGEGTGLLV PDHTPRSWAA ALRTLLDDPA RRTAMGARAA
410 420 430
RRAQGFGWGA TAEATLEVYR RAVQDRAAER GADPR
Length:435
Mass (Da):46,114
Last modified:August 21, 2007 - v1
Checksum:i8DB86DB1F9A8DB24
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000750 Genomic DNA. Translation: ABS02378.1.
RefSeqiWP_012084772.1. NC_009664.2.

Genome annotation databases

EnsemblBacteriaiABS02378; ABS02378; Krad_0890.
KEGGikra:Krad_0890.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000750 Genomic DNA. Translation: ABS02378.1.
RefSeqiWP_012084772.1. NC_009664.2.

3D structure databases

ProteinModelPortaliA6W6D9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi266940.Krad_0890.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABS02378; ABS02378; Krad_0890.
KEGGikra:Krad_0890.

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiPOG091H0AO3.

Enzyme and pathway databases

BioCyciKRAD266940:GI4N-1344-MONOMER.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMSHA_KINRD
AccessioniPrimary (citable) accession number: A6W6D9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: August 21, 2007
Last modified: September 7, 2016
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.