A6W588 (A6W588_KINRD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
DNA topoisomerase I EMBL ABS01977.1

Gene names

Ordered Locus Names:

Krad_0487

Organism

Kineococcus radiotolerans (strain ATCC BAA-149 / DSM 14245 / SRS30216) [Complete proteome] [HAMAP] EMBL ABS01977.1

Taxonomic identifier

266940 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Kineosporiineae › Kineosporiaceae › Kineococcus

Protein attributes

Sequence length	963 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. SAAS SAAS005733
Sequence similarities	Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	A6W588 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 01A775A732F3BD7D	FASTA	963	103,963
	10 20 30 40 50 60 MAGKSQGTRA SGDGLRRLVI VESPSKIDKI STYLGPGYQV EASYGHIRDL PQPSQLPEDV 70 80 90 100 110 120 KKGPYGKFAV DVDHGFEPYY VVDDDKKKKV NELKRALKDA DELILATDRD REGEAIAWHL 130 140 150 160 170 180 VQALEPRVPY RRMVFGEITR EAIQQAVGEL RDIDDRMVDA QETRRILDRL YGYELSPVLW 190 200 210 220 230 240 RKVRAGLSAG RVQSVATRLC VERERERMAF VAAGYADVTG TFTATGEQAG SAFSARLSSL 250 260 270 280 290 300 DGKRVATGRD FGDDGRLKAS SANVAHLDVP AAQALVDGLD GASFAVRGVE EKPYTRRPAA 310 320 330 340 350 360 PFITSTLQQE ASRKLRLNAR QAMRTAQGLY ERGYITYMRT DSVQLSSQAL SAARAQAREL 370 380 390 400 410 420 YGPEYVPDSP RAYASKSKNA QEGHEAIRPA GETFRTPAQV SGELRGDDFR MYDLIWKRTV 430 440 450 460 470 480 ASQMHDARGT TATVRLGATT DDGRDAEFSA SGTIITFRGF LAAYEEGRDA TRDADSEGAG 490 500 510 520 530 540 DEDRRLPQVV AGDPLTASDL ATAGHETSPP ARYTEASLVK ALEEKGIGRP STFASIISTI 550 560 570 580 590 600 VDRGYVTYKG SALVPSWLAF AVTRLLEEHF PKLVDYDFTA GMENDLDAIS RGEAERVDWL 610 620 630 640 650 660 TRFYFGAAGP TAGAVVADGP DGSGGGAALD GIKGLADDLG SIDAREISTI PIGEGIVLRV 670 680 690 700 710 720 GRYGPYLEGP DVETGELLKA SVPEDIAPDE MTVDKARELL TSQSDGDNVL GQDPESGRTI 730 740 750 760 770 780 VARVGRYGPY VTELVEDDAP APAPEEPAEG APKKRAAKKA AKVKPRTGSL FKDMSVDSVT 790 800 810 820 830 840 LEQALQLLSL PRVVGTDPET GDEITAQNGR YGPYLKKGTD SRSLETEDQI FSITLPEVLA 850 860 870 880 890 900 IYAQPKMRRG QVAKPPLKEL GADPESGQPI VIKDGRFGPY ATDGTTNATL RKDDDPETVT 910 920 930 940 950 960 LERAAELLAE KRAKGPAKKT ARKTATKKTA AKTTTGTATV KKTAAKKTAA KKTTARKTAA AES

References

[1]

"Complete sequence of chromosome of Kineococcus radiotolerans SRS30216." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Lykidis A., Bagwell C.E., Shimkets L., Berry C.J., Fliermans C., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-149 / DSM 14245 / SRS30216.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000750 Genomic DNA. Translation: ABS01977.1.
RefSeq	YP_001360241.1. NC_009664.2.
3D structure databases
ProteinModelPortal	A6W588.
ModBase	Search...
Protein-protein interaction databases
STRING	A6W588.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5337756.
GenomeReviews	Gene locus Krad_0487 in contig CP000750_GR.
KEGG	kra:Krad_0487.
NMPDR	fig|266940.1.peg.1786.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0550.
HOGENOM	HBG512935.
OMA	KKVMPRL.
ProtClustDB	CLSK2488184.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A6W588_KINRD

Accession

Primary (citable) accession number: A6W588

Entry history

Integrated into UniProtKB/TrEMBL:	August 21, 2007
Last sequence update:	August 21, 2007
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)