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Protein

Glutamate 5-kinase

Gene

proB

Organism
Marinomonas sp. (strain MWYL1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei16ATPUniRule annotation1
Binding sitei56SubstrateUniRule annotation1
Binding sitei143SubstrateUniRule annotation1
Binding sitei155Substrate; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi175 – 176ATPUniRule annotation2
Nucleotide bindingi217 – 223ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Proline biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMSP400668:G1G9E-4391-MONOMER
UniPathwayiUPA00098; UER00359

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:Mmwyl1_4235
OrganismiMarinomonas sp. (strain MWYL1)
Taxonomic identifieri400668 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaOceanospirillalesOceanospirillaceaeMarinomonas
Proteomesi
  • UP000001113 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001252431 – 374Glutamate 5-kinaseAdd BLAST374

Proteomic databases

PRIDEiA6W351

Interactioni

Protein-protein interaction databases

STRINGi400668.Mmwyl1_4235

Structurei

3D structure databases

ProteinModelPortaliA6W351
SMRiA6W351
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini282 – 360PUAUniRule annotationAdd BLAST79

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CGT Bacteria
COG0263 LUCA
HOGENOMiHOG000246368
KOiK00931
OMAiTHEIRFG
OrthoDBiPOG091H00OF

Family and domain databases

Gene3Di2.30.130.10, 1 hit
3.40.1160.10, 1 hit
HAMAPiMF_00456 ProB, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR001048 Asp/Glu/Uridylate_kinase
IPR001057 Glu/AcGlu_kinase
IPR011529 Glu_5kinase
IPR005715 Glu_5kinase/COase_Synthase
IPR019797 Glutamate_5-kinase_CS
IPR002478 PUA
IPR015947 PUA-like_sf
IPR036974 PUA_sf
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PF01472 PUA, 1 hit
PIRSFiPIRSF000729 GK, 1 hit
PRINTSiPR00474 GLU5KINASE
SMARTiView protein in SMART
SM00359 PUA, 1 hit
SUPFAMiSSF53633 SSF53633, 1 hit
SSF88697 SSF88697, 1 hit
TIGRFAMsiTIGR01027 proB, 1 hit
PROSITEiView protein in PROSITE
PS00902 GLUTAMATE_5_KINASE, 1 hit
PS50890 PUA, 1 hit

Sequencei

Sequence statusi: Complete.

A6W351-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEMREKIAKA QRVVVKIGSA LLTNDGQGLD VARIGLWVAQ IAELRAQGKE
60 70 80 90 100
VVLVSSGSIA AGMKRLGFSS RPTQVNELQA AAAVGQMELV GVYESHFEKH
110 120 130 140 150
GLCTAQILLT HDDLSNRRRY LNARSSLRTL LGFGVVPIIN ENDTVVTDEI
160 170 180 190 200
RFGDNDTLGA LVANLVEADL LIILTDQQGL FDKNPRDHQD ATLISHISAS
210 220 230 240 250
DNRLESMASG GAGVLGSGGM LTKVRAALLA ARSGADTLIA SGREENVIVR
260 270 280 290 300
VASGEMMGTW LQPEHGRVAA RKQWLAGHLK SRGALILDDG AVKALRKEGT
310 320 330 340 350
SLLAVGVKDA QGTFSRGDMV VCVDLQGGLV ARGLVNYSVA ETLKLLGQPS
360 370
SNIGAILGYK GEPELIHRDD LVII
Length:374
Mass (Da):39,810
Last modified:August 21, 2007 - v1
Checksum:iBB843E5F4101381C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000749 Genomic DNA Translation: ABR73130.1
RefSeqiWP_012071895.1, NC_009654.1

Genome annotation databases

EnsemblBacteriaiABR73130; ABR73130; Mmwyl1_4235
KEGGimmw:Mmwyl1_4235

Similar proteinsi

Entry informationi

Entry nameiPROB_MARMS
AccessioniPrimary (citable) accession number: A6W351
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: August 21, 2007
Last modified: May 23, 2018
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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