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A6W351 (PROB_MARMS) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate 5-kinase

EC=2.7.2.11
Alternative name(s):
Gamma-glutamyl kinase
Short name=GK
Gene names
Name:proB
Ordered Locus Names:Mmwyl1_4235
OrganismMarinomonas sp. (strain MWYL1) [Complete proteome] [HAMAP]
Taxonomic identifier400668 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaOceanospirillalesMarinomonas

Protein attributes

Sequence length374 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity. HAMAP-Rule MF_00456

Catalytic activity

ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP-Rule MF_00456

Pathway

Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP-Rule MF_00456

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00456.

Sequence similarities

Belongs to the glutamate 5-kinase family.

Contains 1 PUA domain.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Proline biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processL-proline biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA binding

Inferred from electronic annotation. Source: InterPro

glutamate 5-kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 374374Glutamate 5-kinase HAMAP-Rule MF_00456
PRO_1000125243

Regions

Domain282 – 36079PUA
Nucleotide binding175 – 1762ATP By similarity
Nucleotide binding217 – 2237ATP By similarity

Sites

Binding site161ATP By similarity
Binding site561Substrate By similarity
Binding site1431Substrate By similarity
Binding site1551Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
A6W351 [UniParc].

Last modified August 21, 2007. Version 1.
Checksum: BB843E5F4101381C

FASTA37439,810
        10         20         30         40         50         60 
MEMREKIAKA QRVVVKIGSA LLTNDGQGLD VARIGLWVAQ IAELRAQGKE VVLVSSGSIA 

        70         80         90        100        110        120 
AGMKRLGFSS RPTQVNELQA AAAVGQMELV GVYESHFEKH GLCTAQILLT HDDLSNRRRY 

       130        140        150        160        170        180 
LNARSSLRTL LGFGVVPIIN ENDTVVTDEI RFGDNDTLGA LVANLVEADL LIILTDQQGL 

       190        200        210        220        230        240 
FDKNPRDHQD ATLISHISAS DNRLESMASG GAGVLGSGGM LTKVRAALLA ARSGADTLIA 

       250        260        270        280        290        300 
SGREENVIVR VASGEMMGTW LQPEHGRVAA RKQWLAGHLK SRGALILDDG AVKALRKEGT 

       310        320        330        340        350        360 
SLLAVGVKDA QGTFSRGDMV VCVDLQGGLV ARGLVNYSVA ETLKLLGQPS SNIGAILGYK 

       370 
GEPELIHRDD LVII 

« Hide

References

[1]"Complete sequence of Marinomonas sp. MWYL1."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Kim E., Johnston A.W.B., Todd J.D., Rogers R., Wexler M., Bond P.L., Li Y., Richardson P.
Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MWYL1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000749 Genomic DNA. Translation: ABR73130.1.
RefSeqYP_001343065.1. NC_009654.1.

3D structure databases

ProteinModelPortalA6W351.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING400668.Mmwyl1_4235.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABR73130; ABR73130; Mmwyl1_4235.
GeneID5367722.
KEGGmmw:Mmwyl1_4235.
PATRIC22472159. VBIMarSp124341_4391.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0263.
HOGENOMHOG000246368.
KOK00931.
OMAPPTIASK.
OrthoDBEOG6PGK7G.

Enzyme and pathway databases

BioCycMSP400668:GHKD-4338-MONOMER.
UniPathwayUPA00098; UER00359.

Family and domain databases

Gene3D2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPMF_00456. ProB.
InterProIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view]
PfamPF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view]
PIRSFPIRSF000729. GK. 1 hit.
PRINTSPR00474. GLU5KINASE.
SMARTSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsTIGR01027. proB. 1 hit.
PROSITEPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePROB_MARMS
AccessionPrimary (citable) accession number: A6W351
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: August 21, 2007
Last modified: February 19, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways