SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

A6W346

- SYI_MARMS

UniProt

A6W346 - SYI_MARMS

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Isoleucine--tRNA ligase
Gene
ileS, Mmwyl1_4230
Organism
Marinomonas sp. (strain MWYL1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei563 – 5631Aminoacyl-adenylate By similarity
Binding sitei607 – 6071ATP By similarity
Metal bindingi902 – 9021Zinc By similarity
Metal bindingi905 – 9051Zinc By similarity
Metal bindingi922 – 9221Zinc By similarity
Metal bindingi925 – 9251Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMSP400668:GHKD-4333-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Mmwyl1_4230
OrganismiMarinomonas sp. (strain MWYL1)
Taxonomic identifieri400668 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaOceanospirillalesMarinomonas
ProteomesiUP000001113: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 940940Isoleucine--tRNA ligaseUniRule annotation
PRO_1000088548Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi400668.Mmwyl1_4230.

Structurei

3D structure databases

ProteinModelPortaliA6W346.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotation
Add
BLAST
Motifi604 – 6085"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiLGHRVHY.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6W346-1 [UniParc]FASTAAdd to Basket

« Hide

MSDYKPTLNL PDTDFPMRGD LAKREPAMLK RWQDMDLYQK VRDVTKGRKS    50
FILHDGPPYA NGSIHIGHAV NKILKDIIVK SKTVSGFDAP YIPGWDCHGL 100
PIEHKVEQLI GKAGTKVSYK EFRAKCREYA YTQIEEQKKD FIRLGVMGDW 150
ENPYLTMNFQ TEANIVRALG KIAENGHLVK GFKPVYWSVV GGSALAEAEV 200
EYQDKTSLSL DVRYAPQDEA ALLAKFSAVE GEGKVSVVIW TTTPWTLPAS 250
QAVSIHPEFN YALVEVDMGL GKERLILAED MVASVMSRYG VTDFRIVGRT 300
VGAELAGTVL NHPFLQRDIP VILGEHVTTE AGTGCVHTAP DHGVDDFNVG 350
RENGIGTINL VQDNGVYSDA AGEFAGLHVY KVDDAVLEAL NRNNALVFES 400
KIFHSYPHCW RTKTPLIFRA TPQWFISMTK EGLLDSAKHA VEGVKWVPSW 450
GQNRMEGMLN NSPDWCVSRQ RTWGVPIALF INKETQELHP ETPRLIEEVA 500
KRIEVEGIDA WFEMEAEELL GADAEKYSKV TDTLDVWFDS GVTHYSVIDQ 550
RDELSFPADL YLEGSDQHRG WFQSSLKTSI AIRGVPPYKQ VLTHGFTVDG 600
DGRKMSKSLG NVLSPQKVMD TLGADIIRLW VAATDYTTEM TVSDEILKRV 650
ADSYRRIRNT ARFMMANLNG FNPATDMVPA KDMIALDRWI VDRAALLQKE 700
LNTAYNEYQF HTVNQKIQNF CSVDLGGFYL DVIKDRQYTT QKDSLARRSA 750
QTALYHVIEA FSRWIAPILS FTADEIWQTL PGERGESVFL ETWYEGLEEL 800
TGDEAMGREF WKQVLEAKVA TNKVLEAARS EGKMKASLSA DITLYCDDAL 850
QTTLNRLGEE LRFVLIASDV KVLPLSQAGE DAVATDLDGL KVHVELSKYT 900
KCVRCWHHRE EVGKRDAHPE LCDRCISNLP DGEGEQRLYA 940
Length:940
Mass (Da):105,701
Last modified:August 21, 2007 - v1
Checksum:iC9EE7AC5231EA419
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000749 Genomic DNA. Translation: ABR73125.1.
RefSeqiWP_012071890.1. NC_009654.1.
YP_001343060.1. NC_009654.1.

Genome annotation databases

EnsemblBacteriaiABR73125; ABR73125; Mmwyl1_4230.
GeneIDi5365381.
KEGGimmw:Mmwyl1_4230.
PATRICi22472149. VBIMarSp124341_4386.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000749 Genomic DNA. Translation: ABR73125.1 .
RefSeqi WP_012071890.1. NC_009654.1.
YP_001343060.1. NC_009654.1.

3D structure databases

ProteinModelPortali A6W346.
ModBasei Search...

Protein-protein interaction databases

STRINGi 400668.Mmwyl1_4230.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABR73125 ; ABR73125 ; Mmwyl1_4230 .
GeneIDi 5365381.
KEGGi mmw:Mmwyl1_4230.
PATRICi 22472149. VBIMarSp124341_4386.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi LGHRVHY.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci MSP400668:GHKD-4333-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MWYL1.

Entry informationi

Entry nameiSYI_MARMS
AccessioniPrimary (citable) accession number: A6W346
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: August 21, 2007
Last modified: September 3, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi