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Protein

Class B acid phosphatase

Gene

aphA

Organism
Marinomonas sp. (strain MWYL1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Dephosphorylates several organic phosphate monoesters. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds (By similarity).By similarity

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.By similarity

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei70NucleophileBy similarity1
Metal bindingi70MagnesiumBy similarity1
Active sitei72Proton donorBy similarity1
Metal bindingi72Magnesium; via carbonyl oxygenBy similarity1
Binding sitei176SubstrateBy similarity1
Metal bindingi191MagnesiumBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Class B acid phosphataseBy similarity (EC:3.1.3.2By similarityImported)
Short name:
CBAPBy similarity
Gene namesi
Name:aphABy similarity
Ordered Locus Names:Mmwyl1_3860
OrganismiMarinomonas sp. (strain MWYL1)
Taxonomic identifieri400668 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaOceanospirillalesMarinomonas
Proteomesi
  • UP000001113 Componenti: Chromosome

Subcellular locationi

  • Periplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_500025971223 – 236Class B acid phosphataseSequence analysisAdd BLAST214

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi400668.Mmwyl1_3860.

Structurei

3D structure databases

ProteinModelPortaliA6W230.
SMRiA6W230.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni138 – 139Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the class B bacterial acid phosphatase family.By similarity

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105F0S. Bacteria.
COG3700. LUCA.
HOGENOMiHOG000270623.
KOiK03788.
OMAiPAFCANP.
OrthoDBiPOG091H06BK.

Family and domain databases

CDDicd07499. HAD_CBAP. 1 hit.
Gene3Di3.40.50.1000. 1 hit.
InterProiView protein in InterPro
IPR005519. Acid_phosphat_B-like.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
PfamiView protein in Pfam
PF03767. Acid_phosphat_B. 1 hit.
PIRSFiPIRSF017818. Acid_Ptase_B. 1 hit.
SFLDiSFLDG01127. C1.3:_Acid_Phosphatase_Like. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01672. AphA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A6W230-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNHTLRSITL VLACVASLSA NADPKTPGTH KGYSTIDMTT AGMPADVKMI
60 70 80 90 100
SVADIQKELQ GKAPFAVGFD IDDTTLFSTP VFYRGQQEFS PNGYSYLTNQ
110 120 130 140 150
DFWDKANCGW DAFSMPKNIA KELIAMHQER GDSIYFITGR TGSDCNFTTE
160 170 180 190 200
YLKKTFDIKD MHDVIFAGSS RTEYTKTKYI KNNDIKIYYG DADGDIISAR
210 220 230
DAGAEGIRVM RAANSSYTPI PKNGIYGERV LKDSQY
Length:236
Mass (Da):26,223
Last modified:August 21, 2007 - v1
Checksum:i54217BA69A47D9D1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000749 Genomic DNA. Translation: ABR72759.1.

Genome annotation databases

EnsemblBacteriaiABR72759; ABR72759; Mmwyl1_3860.
KEGGimmw:Mmwyl1_3860.

Similar proteinsi

Entry informationi

Entry nameiAPHA_MARMS
AccessioniPrimary (citable) accession number: A6W230
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: August 21, 2007
Last modified: August 30, 2017
This is version 58 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families