A6W1W3 (TDH_MARMS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-threonine 3-dehydrogenase
EC=1.1.1.103

Gene names

Name:	tdh
Ordered Locus Names:	Mmwyl1_3793

Organism

Marinomonas sp. (strain MWYL1) [Complete proteome] [HAMAP]

Taxonomic identifier

400668 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Oceanospirillaceae › Marinomonas

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-threonine + NAD+ = L-2-amino-3-oxobutanoate + NADH. HAMAP-Rule MF_00627
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Amino-acid degradation; L-threonine degradation via oxydo-reductase pathway; glycine from L-threonine: step 1/2. HAMAP-Rule MF_00627
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	L-threonine catabolic process to glycine Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	L-threonine 3-dehydrogenase activity Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	L-threonine 3-dehydrogenase HAMAP-Rule MF_00627		PRO_1000082612
Sites
	Metal binding	38	1	Zinc 1; catalytic By similarity
	Metal binding	63	1	Zinc 1; catalytic By similarity
	Metal binding	93	1	Zinc 2 By similarity
	Metal binding	96	1	Zinc 2 By similarity
	Metal binding	99	1	Zinc 2 By similarity
	Metal binding	107	1	Zinc 2 By similarity
	Metal binding	148	1	Zinc 1; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6W1W3 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 9180E66CFDDEE13F	FASTA	341	37,328
	10 20 30 40 50 60 MKTLAKLHAE KGIWMTDVPH PDCGHNDVVI KISKTAICGT DMHIYQWDDW AQNTIPVPMT 70 80 90 100 110 120 VGHEFVGVIT EVGPEVSGFK IGDRVSGEGH ITCGHCRNCR AGRRHLCRKT LGVGVNRTGA 130 140 150 160 170 180 FAEYLVIPAS NAFKIPNNIS DDMAAIFDPF GNATHTALSF DLIGEDVLIT GAGPIGAMAA 190 200 210 220 230 240 AIAKHVGARN VVITDVNDFR LDLAKKMGAT RTVNVSRESL KDVMTEIDMH EGFDVGLEMS 250 260 270 280 290 300 GNDMAFRSML ECMNHGGKIA MLGIPGKDTL IDWNQVIFKG LIIKGIYGRE MYETWYKMVA 310 320 330 340 MLQSGLDISP IITHRFKVDE FQQGFDTMGS GLSGKVILDW N

References

[1]

"Complete sequence of Marinomonas sp. MWYL1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Johnston A.W.B., Todd J.D., Rogers R., Wexler M., Bond P.L., Li Y., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MWYL1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000749 Genomic DNA. Translation: ABR72692.1.
RefSeq	YP_001342627.1. NC_009654.1.
3D structure databases
ProteinModelPortal	A6W1W3.
ModBase	Search...
Protein-protein interaction databases
STRING	400668.Mmwyl1_3793.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABR72692; ABR72692; Mmwyl1_3793.
GeneID	5365579.
KEGG	mmw:Mmwyl1_3793.
PATRIC	22471209. VBIMarSp124341_3927.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1063.
HOGENOM	HOG000294686.
KO	K00060.
OMA	MSIDWNK.
ProtClustDB	PRK05396.
Enzyme and pathway databases
BioCyc	MSP400668:GHKD-3904-MONOMER.
UniPathway	UPA00046; UER00505.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
HAMAP	MF_00627. Thr_dehydrog.
InterPro	IPR013149. ADH_C. IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn-type. IPR002328. ADH_Zn_CS. IPR011032. GroES-like. IPR004627. L-Threonine_3-DHase. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11695. PTHR11695. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
SUPFAM	SSF50129. GroES_like. 1 hit.
TIGRFAMs	TIGR00692. tdh. 1 hit.
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TDH_MARMS

Accession

Primary (citable) accession number: A6W1W3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	August 21, 2007
Last modified:	May 1, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program