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A6W0Y1

- BIOB_MARMS

UniProt

A6W0Y1 - BIOB_MARMS

Protein

Biotin synthase

Gene

bioB

Organism
Marinomonas sp. (strain MWYL1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 49 (01 Oct 2014)
      Sequence version 2 (28 Jul 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi59 – 591Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi63 – 631Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi66 – 661Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi103 – 1031Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi134 – 1341Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi194 – 1941Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi266 – 2661Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciMSP400668:GHKD-3526-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:Mmwyl1_3457
    OrganismiMarinomonas sp. (strain MWYL1)
    Taxonomic identifieri400668 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaOceanospirillalesMarinomonas
    ProteomesiUP000001113: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 351351Biotin synthasePRO_0000381456Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi400668.Mmwyl1_3457.

    Structurei

    3D structure databases

    ProteinModelPortaliA6W0Y1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239957.
    KOiK01012.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A6W0Y1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSINNISAPR FDWTHAEIKA LFDLPFMDLM FRAQTVHREN FAPNEVQVST    50
    LLSIKTGACP EDCKYCPQSG HYNTELEKEK LMEVQKVLEE AALAKEKGAS 100
    RFCMGAAWKH PSEKDFPYVL EMIKGVKSLG LESCVTLGSL NDDQAKRLSE 150
    VGLDYYNHNL DTSAEFYDSI ITTRTYQDRL DTLSRVRDSG IKLCCGGIMG 200
    MGEEQKDRVG LLRQLSQMTP HPESVPINML VKVEGTPLEN VDDLDTFEFI 250
    RTIAVARILM PKSHVRLSAG RQGMNEQTQA LCFMAGANSI FYGEKLLTTG 300
    NPEADKDMAL FANLGINPEK REEHSDAAVA EAIAAQVVKQ EESKLFYEAS 350
    V 351
    Length:351
    Mass (Da):39,189
    Last modified:July 28, 2009 - v2
    Checksum:iADA81CC32F0CDC27
    GO

    Sequence cautioni

    The sequence ABR72360.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000749 Genomic DNA. Translation: ABR72360.1. Different initiation.
    RefSeqiWP_012071127.1. NC_009654.1.
    YP_001342295.1. NC_009654.1.

    Genome annotation databases

    EnsemblBacteriaiABR72360; ABR72360; Mmwyl1_3457.
    GeneIDi5367317.
    KEGGimmw:Mmwyl1_3457.
    PATRICi22470459. VBIMarSp124341_3574.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000749 Genomic DNA. Translation: ABR72360.1 . Different initiation.
    RefSeqi WP_012071127.1. NC_009654.1.
    YP_001342295.1. NC_009654.1.

    3D structure databases

    ProteinModelPortali A6W0Y1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 400668.Mmwyl1_3457.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABR72360 ; ABR72360 ; Mmwyl1_3457 .
    GeneIDi 5367317.
    KEGGi mmw:Mmwyl1_3457.
    PATRICi 22470459. VBIMarSp124341_3574.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239957.
    KOi K01012.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci MSP400668:GHKD-3526-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MWYL1.

    Entry informationi

    Entry nameiBIOB_MARMS
    AccessioniPrimary (citable) accession number: A6W0Y1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: July 28, 2009
    Last modified: October 1, 2014
    This is version 49 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3