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A6W0Y1

- BIOB_MARMS

UniProt

A6W0Y1 - BIOB_MARMS

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Protein
Biotin synthase
Gene
bioB, Mmwyl1_3457
Organism
Marinomonas sp. (strain MWYL1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi59 – 591Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi63 – 631Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi66 – 661Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi103 – 1031Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi134 – 1341Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi194 – 1941Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi266 – 2661Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMSP400668:GHKD-3526-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:Mmwyl1_3457
OrganismiMarinomonas sp. (strain MWYL1)
Taxonomic identifieri400668 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaOceanospirillalesMarinomonas
ProteomesiUP000001113: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 351351Biotin synthaseUniRule annotation
PRO_0000381456Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi400668.Mmwyl1_3457.

Structurei

3D structure databases

ProteinModelPortaliA6W0Y1.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

A6W0Y1-1 [UniParc]FASTAAdd to Basket

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MSINNISAPR FDWTHAEIKA LFDLPFMDLM FRAQTVHREN FAPNEVQVST    50
LLSIKTGACP EDCKYCPQSG HYNTELEKEK LMEVQKVLEE AALAKEKGAS 100
RFCMGAAWKH PSEKDFPYVL EMIKGVKSLG LESCVTLGSL NDDQAKRLSE 150
VGLDYYNHNL DTSAEFYDSI ITTRTYQDRL DTLSRVRDSG IKLCCGGIMG 200
MGEEQKDRVG LLRQLSQMTP HPESVPINML VKVEGTPLEN VDDLDTFEFI 250
RTIAVARILM PKSHVRLSAG RQGMNEQTQA LCFMAGANSI FYGEKLLTTG 300
NPEADKDMAL FANLGINPEK REEHSDAAVA EAIAAQVVKQ EESKLFYEAS 350
V 351
Length:351
Mass (Da):39,189
Last modified:July 28, 2009 - v2
Checksum:iADA81CC32F0CDC27
GO

Sequence cautioni

The sequence ABR72360.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000749 Genomic DNA. Translation: ABR72360.1. Different initiation.
RefSeqiWP_012071127.1. NC_009654.1.
YP_001342295.1. NC_009654.1.

Genome annotation databases

EnsemblBacteriaiABR72360; ABR72360; Mmwyl1_3457.
GeneIDi5367317.
KEGGimmw:Mmwyl1_3457.
PATRICi22470459. VBIMarSp124341_3574.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000749 Genomic DNA. Translation: ABR72360.1 . Different initiation.
RefSeqi WP_012071127.1. NC_009654.1.
YP_001342295.1. NC_009654.1.

3D structure databases

ProteinModelPortali A6W0Y1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 400668.Mmwyl1_3457.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABR72360 ; ABR72360 ; Mmwyl1_3457 .
GeneIDi 5367317.
KEGGi mmw:Mmwyl1_3457.
PATRICi 22470459. VBIMarSp124341_3574.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci MSP400668:GHKD-3526-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MWYL1.

Entry informationi

Entry nameiBIOB_MARMS
AccessioniPrimary (citable) accession number: A6W0Y1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: September 3, 2014
This is version 48 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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