Reviewed, UniProtKB/Swiss-Prot A6VXM3 (PDXB_MARMS)

Names and origin

Protein names

Recommended name:
    Erythronate-4-phosphate dehydrogenase
    EC=1.1.1.290

Gene names

Name:	pdxB
Ordered Locus Names:	Mmwyl1_2280

Organism

Marinomonas sp. (strain MWYL1) [Complete proteome] [HAMAP]

Taxonomic identifier

400668 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Marinomonas

Protein attributes

Sequence length	380 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of erythronate-4-phosphate to 3-hydroxy-2-oxo-4-phosphonooxybutanoate By similarity.
Catalytic activity	4-phospho-D-erythronate + NAD+ = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + NADH. HAMAP MF_01825
Pathway	Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 2/5. HAMAP MF_01825
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. PdxB subfamily.

Ontologies

Keywords
Biological process	Pyridoxine biosynthesis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pyridoxine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4-phosphoerythronate dehydrogenase activity Inferred from electronic annotation. Source: EC NAD or NADH binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 380	380	Erythronate-4-phosphate dehydrogenase HAMAP MF_01825		PRO_1000088422
Sites
	Active site	207	1	By similarity
	Active site	237	1	By similarity
	Active site	254	1	Proton donor By similarity
	Binding site	45	1	Substrate By similarity
	Binding site	66	1	Substrate By similarity
	Binding site	146	1	NAD By similarity
	Binding site	232	1	NAD By similarity
	Binding site	257	1	NAD; via amide nitrogen By similarity
	Binding site	258	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6VXM3-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: BC0C7CB0DFDA8507	FASTA	380	41,881
	10 20 30 40 50 60 MKIIADENMP NAKVLFSHLG DVELVNGRTL THEQVREADV LLVRSVTKVT KELLEGSSVR 70 80 90 100 110 120 FVGSATIGVD HIDLDYLSKA NIGFSSAPGC NAEAVADYVF SALSHLYLTK KINWLSKKIG 130 140 150 160 170 180 VIGYGNVGKT VYTRFANMGC QVHVYDPIRE KEGGSANFVS LDEILSCDVI SLHAPLTHTG 190 200 210 220 230 240 SYPTKGMIGR KELAKLSAGV TIISAGRGGV IDESALFDRH KQLNGNLHLV LDVWDGEPAI 250 260 270 280 290 300 NQKLIAIVDI ATPHIAGYSK QGREKGTWMV YQALCQYLAL DANVISKHDA ISAGWLSFVN 310 320 330 340 350 360 VSAEEPQEEM LARSMHAIYD VSRDDIRLRF KYRENKEKNV FDWLRKHYVE RDEFNTCIIG 370 380 VSSSDASNLM SAAGFSVDNK

References

[1]

"Complete sequence of Marinomonas sp. MWYL1." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Johnston A.W.B., Todd J.D., Rogers R., Wexler M., Bond P.L., Li Y., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000749 Genomic DNA. Translation: ABR71202.1.
RefSeq	YP_001341137.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5365538.
GenomeReviews	Gene locus Mmwyl1_2280 in contig CP000749_GR.
KEGG	mmw:Mmwyl1_2280.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A6VXM3. SAPGCNA.
Family and domain databases
HAMAP	MF_01825. [Tree]
InterPro	IPR006139. D-isomer_2_OHA_DH. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. 1 hit. PS00670. D_2_HYDROXYACID_DH_2. False negative. PS00671. D_2_HYDROXYACID_DH_3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDXB_MARMS

Accession

Primary (citable) accession number: A6VXM3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	August 21, 2007
Last modified:	June 16, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)