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A6VQY7 (NAPA_ACTSZ) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:Asuc_2038
OrganismActinobacillus succinogenes (strain ATCC 55618 / 130Z) [Complete proteome] [HAMAP]
Taxonomic identifier339671 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus

Protein attributes

Sequence length826 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3232Tat-type signal Potential
Chain33 – 826794Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_5000259017

Regions

Domain37 – 93574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding441Iron-sulfur (4Fe-4S) By similarity
Metal binding471Iron-sulfur (4Fe-4S) By similarity
Metal binding511Iron-sulfur (4Fe-4S) By similarity
Metal binding791Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A6VQY7 [UniParc].

Last modified August 21, 2007. Version 1.
Checksum: 26BCEDE3801039D8

FASTA82693,338
        10         20         30         40         50         60 
MELNRRDFMK ANAAIAAAAA AGITIPVKNV QAADDGIRWD KAPCRYCGTG CSVLVGTKDG 

        70         80         90        100        110        120 
RVVATQGDPD AEVNRGLNCI KGYFLSKIMY GADRVQQPLL RMKDGKFDKE GDFTPISWEQ 

       130        140        150        160        170        180 
AFTIMAEKIK DILKKKEPNA IGMFSSGQTT IYEGYAKVKL WKAGLRSNTI DPNARHCMAS 

       190        200        210        220        230        240 
AAVAFMRTFG MDEPMGCYND IEKADTFVLW GSNMAEMHPI LWSRISDRRL SGDNVKVVVM 

       250        260        270        280        290        300 
STYEHRSFEL ADTPIIFKPQ SDLAILNYIA NYIIQNDKVD WDFVNKHTKF KRGETNIGYG 

       310        320        330        340        350        360 
LRPEHPLQQG TNAKTAGKMY DSDFEEFKKI VSEYTLDKAH EISGVPKDQL ETLAKMYADP 

       370        380        390        400        410        420 
EQNLVSYWTM GFNQHTRGVW VNQMMYNVHL LTGKISKPGC GPFSLTGQPS ACGTAREVGT 

       430        440        450        460        470        480 
FIHRLPADMV VTNPKHVAKA EEIWQLPAGT IPTVPGFHAV AQSRALKDGK LNFLWQMCTN 

       490        500        510        520        530        540 
NMQGGPNINE ETFPGWRNPE NFIVVSDPYP STSAVAADLI LPTCMWVEKE GGYGNAERRT 

       550        560        570        580        590        600 
QLWRQQVKGP GESRSDLWQI VEFSKYFKTD EVWSEELLAQ KPEYRGKTLY EVLYKNGEVD 

       610        620        630        640        650        660 
KFQVPTNIPG YINDEADHFG FYLQKGLFEE YASFGRGHGH DLAPFDVYHQ VRGLRWPVVD 

       670        680        690        700        710        720 
GKETLWRYRE GYDPYVKSGE EVAFYGNPDN KAIILGVPYE APAESPDEEY DLWLCTGRVL 

       730        740        750        760        770        780 
EHWHTGTMTR RVPELHKAFP NNLVWMHPTD AKKRGLRHGD KVKLITRRGE MISHLDTRGR 

       790        800        810        820 
NKTPEGLIFT TFFDAGQLTN KLTLDATDPI SFETDYKKCA VKVVKA 

« Hide

References

[1]"Complete sequence of Actinobacillus succinogenes 130Z."
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Lowry S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Vieille C., Richardson P.
Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 55618 / 130Z.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000746 Genomic DNA. Translation: ABR75384.1.
RefSeqYP_001345319.1. NC_009655.1.

3D structure databases

ProteinModelPortalA6VQY7.
SMRA6VQY7. Positions 35-825.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING339671.Asuc_2038.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABR75384; ABR75384; Asuc_2038.
GeneID5349259.
KEGGasu:Asuc_2038.
PATRIC20762550. VBIActSuc117883_2139.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMARRTHVWH.
OrthoDBEOG6CVV7G.
ProtClustDBPRK13532.

Enzyme and pathway databases

BioCycASUC339671:GHDX-2119-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_ACTSZ
AccessionPrimary (citable) accession number: A6VQY7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: August 21, 2007
Last modified: February 19, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families