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A6VQY7

- NAPA_ACTSZ

UniProt

A6VQY7 - NAPA_ACTSZ

Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Actinobacillus succinogenes (strain ATCC 55618 / 130Z)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 1 (21 Aug 2007)
      Previous versions | rss
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    Functioni

    Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi44 – 441Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi47 – 471Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi51 – 511Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi79 – 791Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. electron carrier activity Source: UniProtKB-HAMAP
    3. iron ion binding Source: UniProtKB-HAMAP
    4. molybdenum ion binding Source: InterPro
    5. nitrate reductase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
    2. nitrate assimilation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Enzyme and pathway databases

    BioCyciASUC339671:GHDX-2119-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
    Gene namesi
    Name:napAUniRule annotation
    Ordered Locus Names:Asuc_2038
    OrganismiActinobacillus succinogenes (strain ATCC 55618 / 130Z)
    Taxonomic identifieri339671 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus
    ProteomesiUP000001114: Chromosome

    Subcellular locationi

    Periplasm UniRule annotation

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3232Tat-type signalUniRule annotationAdd
    BLAST
    Chaini33 – 826794Periplasmic nitrate reductasePRO_5000259017Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

    Interactioni

    Subunit structurei

    Interacts with NapB.UniRule annotation

    Protein-protein interaction databases

    STRINGi339671.Asuc_2038.

    Structurei

    3D structure databases

    ProteinModelPortaliA6VQY7.
    SMRiA6VQY7. Positions 35-825.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini37 – 93574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0243.
    HOGENOMiHOG000031441.
    KOiK02567.
    OMAiHPKTRVA.
    OrthoDBiEOG6CVV7G.

    Family and domain databases

    HAMAPiMF_01630. Nitrate_reduct.
    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    TIGRFAMsiTIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A6VQY7-1 [UniParc]FASTAAdd to Basket

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    MELNRRDFMK ANAAIAAAAA AGITIPVKNV QAADDGIRWD KAPCRYCGTG    50
    CSVLVGTKDG RVVATQGDPD AEVNRGLNCI KGYFLSKIMY GADRVQQPLL 100
    RMKDGKFDKE GDFTPISWEQ AFTIMAEKIK DILKKKEPNA IGMFSSGQTT 150
    IYEGYAKVKL WKAGLRSNTI DPNARHCMAS AAVAFMRTFG MDEPMGCYND 200
    IEKADTFVLW GSNMAEMHPI LWSRISDRRL SGDNVKVVVM STYEHRSFEL 250
    ADTPIIFKPQ SDLAILNYIA NYIIQNDKVD WDFVNKHTKF KRGETNIGYG 300
    LRPEHPLQQG TNAKTAGKMY DSDFEEFKKI VSEYTLDKAH EISGVPKDQL 350
    ETLAKMYADP EQNLVSYWTM GFNQHTRGVW VNQMMYNVHL LTGKISKPGC 400
    GPFSLTGQPS ACGTAREVGT FIHRLPADMV VTNPKHVAKA EEIWQLPAGT 450
    IPTVPGFHAV AQSRALKDGK LNFLWQMCTN NMQGGPNINE ETFPGWRNPE 500
    NFIVVSDPYP STSAVAADLI LPTCMWVEKE GGYGNAERRT QLWRQQVKGP 550
    GESRSDLWQI VEFSKYFKTD EVWSEELLAQ KPEYRGKTLY EVLYKNGEVD 600
    KFQVPTNIPG YINDEADHFG FYLQKGLFEE YASFGRGHGH DLAPFDVYHQ 650
    VRGLRWPVVD GKETLWRYRE GYDPYVKSGE EVAFYGNPDN KAIILGVPYE 700
    APAESPDEEY DLWLCTGRVL EHWHTGTMTR RVPELHKAFP NNLVWMHPTD 750
    AKKRGLRHGD KVKLITRRGE MISHLDTRGR NKTPEGLIFT TFFDAGQLTN 800
    KLTLDATDPI SFETDYKKCA VKVVKA 826
    Length:826
    Mass (Da):93,338
    Last modified:August 21, 2007 - v1
    Checksum:i26BCEDE3801039D8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000746 Genomic DNA. Translation: ABR75384.1.
    RefSeqiYP_001345319.1. NC_009655.1.

    Genome annotation databases

    EnsemblBacteriaiABR75384; ABR75384; Asuc_2038.
    GeneIDi5349259.
    KEGGiasu:Asuc_2038.
    PATRICi20762550. VBIActSuc117883_2139.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000746 Genomic DNA. Translation: ABR75384.1 .
    RefSeqi YP_001345319.1. NC_009655.1.

    3D structure databases

    ProteinModelPortali A6VQY7.
    SMRi A6VQY7. Positions 35-825.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 339671.Asuc_2038.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABR75384 ; ABR75384 ; Asuc_2038 .
    GeneIDi 5349259.
    KEGGi asu:Asuc_2038.
    PATRICi 20762550. VBIActSuc117883_2139.

    Phylogenomic databases

    eggNOGi COG0243.
    HOGENOMi HOG000031441.
    KOi K02567.
    OMAi HPKTRVA.
    OrthoDBi EOG6CVV7G.

    Enzyme and pathway databases

    BioCyci ASUC339671:GHDX-2119-MONOMER.

    Family and domain databases

    HAMAPi MF_01630. Nitrate_reduct.
    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    TIGRFAMsi TIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A genomic perspective on the potential of Actinobacillus succinogenes for industrial succinate production."
      McKinlay J.B., Laivenieks M., Schindler B.D., McKinlay A.A., Siddaramappa S., Challacombe J.F., Lowry S.R., Clum A., Lapidus A.L., Burkhart K.B., Harkins V., Vieille C.
      BMC Genomics 11:680-680(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 55618 / 130Z.

    Entry informationi

    Entry nameiNAPA_ACTSZ
    AccessioniPrimary (citable) accession number: A6VQY7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 26, 2008
    Last sequence update: August 21, 2007
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3