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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Actinobacillus succinogenes (strain ATCC 55618 / 130Z)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.UniRule annotation

Catalytic activityi

2 ferrocytochrome + nitrate + 2 H+ = 2 ferricytochrome + nitrite.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi44Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi47Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi51Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi79Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei81Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei148Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei173Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei177Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei370Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei374Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei480Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei529Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei556Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei792Substrate; via amide nitrogenUniRule annotation1
Binding sitei800Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei817Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Nitrate assimilation, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciASUC339671:G1G9D-2131-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.9.6.1UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:Asuc_2038
OrganismiActinobacillus succinogenes (strain ATCC 55618 / 130Z)
Taxonomic identifieri339671 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus
Proteomesi
  • UP000001114 Componenti: Chromosome

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 32Tat-type signalUniRule annotationAdd BLAST32
ChainiPRO_500025901733 – 826Periplasmic nitrate reductaseUniRule annotationAdd BLAST794

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Component of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB.UniRule annotation

Protein-protein interaction databases

STRINGi339671.Asuc_2038

Structurei

3D structure databases

ProteinModelPortaliA6VQY7
SMRiA6VQY7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini37 – 934Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni210 – 217Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation8
Regioni241 – 245Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation5
Regioni260 – 262Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation3
Regioni506 – 507Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation2
Regioni716 – 725Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation10

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QIW Bacteria
COG0243 LUCA
HOGENOMiHOG000031441
KOiK02567
OMAiTQHWRQQ
OrthoDBiPOG091H060P

Family and domain databases

HAMAPiMF_01630 Nitrate_reduct_NapA, 1 hit
InterProiView protein in InterPro
IPR009010 Asp_de-COase-like_dom_sf
IPR006657 MoPterin_dinucl-bd_dom
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR027467 MopterinOxRdtase_cofactor_BS
IPR010051 Periplasm_NO3_reductase_lsu
IPR006311 TAT_signal
IPR019546 TAT_signal_bac_arc
PANTHERiPTHR11615:SF123 PTHR11615:SF123, 1 hit
PfamiView protein in Pfam
PF04879 Molybdop_Fe4S4, 1 hit
PF00384 Molybdopterin, 1 hit
PF01568 Molydop_binding, 1 hit
PF10518 TAT_signal, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
TIGRFAMsiTIGR01706 NAPA, 1 hit
TIGR01409 TAT_signal_seq, 1 hit
PROSITEiView protein in PROSITE
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00551 MOLYBDOPTERIN_PROK_1, 1 hit
PS51318 TAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A6VQY7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELNRRDFMK ANAAIAAAAA AGITIPVKNV QAADDGIRWD KAPCRYCGTG
60 70 80 90 100
CSVLVGTKDG RVVATQGDPD AEVNRGLNCI KGYFLSKIMY GADRVQQPLL
110 120 130 140 150
RMKDGKFDKE GDFTPISWEQ AFTIMAEKIK DILKKKEPNA IGMFSSGQTT
160 170 180 190 200
IYEGYAKVKL WKAGLRSNTI DPNARHCMAS AAVAFMRTFG MDEPMGCYND
210 220 230 240 250
IEKADTFVLW GSNMAEMHPI LWSRISDRRL SGDNVKVVVM STYEHRSFEL
260 270 280 290 300
ADTPIIFKPQ SDLAILNYIA NYIIQNDKVD WDFVNKHTKF KRGETNIGYG
310 320 330 340 350
LRPEHPLQQG TNAKTAGKMY DSDFEEFKKI VSEYTLDKAH EISGVPKDQL
360 370 380 390 400
ETLAKMYADP EQNLVSYWTM GFNQHTRGVW VNQMMYNVHL LTGKISKPGC
410 420 430 440 450
GPFSLTGQPS ACGTAREVGT FIHRLPADMV VTNPKHVAKA EEIWQLPAGT
460 470 480 490 500
IPTVPGFHAV AQSRALKDGK LNFLWQMCTN NMQGGPNINE ETFPGWRNPE
510 520 530 540 550
NFIVVSDPYP STSAVAADLI LPTCMWVEKE GGYGNAERRT QLWRQQVKGP
560 570 580 590 600
GESRSDLWQI VEFSKYFKTD EVWSEELLAQ KPEYRGKTLY EVLYKNGEVD
610 620 630 640 650
KFQVPTNIPG YINDEADHFG FYLQKGLFEE YASFGRGHGH DLAPFDVYHQ
660 670 680 690 700
VRGLRWPVVD GKETLWRYRE GYDPYVKSGE EVAFYGNPDN KAIILGVPYE
710 720 730 740 750
APAESPDEEY DLWLCTGRVL EHWHTGTMTR RVPELHKAFP NNLVWMHPTD
760 770 780 790 800
AKKRGLRHGD KVKLITRRGE MISHLDTRGR NKTPEGLIFT TFFDAGQLTN
810 820
KLTLDATDPI SFETDYKKCA VKVVKA
Length:826
Mass (Da):93,338
Last modified:August 21, 2007 - v1
Checksum:i26BCEDE3801039D8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000746 Genomic DNA Translation: ABR75384.1
RefSeqiWP_012073760.1, NC_009655.1

Genome annotation databases

EnsemblBacteriaiABR75384; ABR75384; Asuc_2038
KEGGiasu:Asuc_2038

Similar proteinsi

Entry informationi

Entry nameiNAPA_ACTSZ
AccessioniPrimary (citable) accession number: A6VQY7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: August 21, 2007
Last modified: March 28, 2018
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health