Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

A6VQY7

- NAPA_ACTSZ

UniProt

A6VQY7 - NAPA_ACTSZ

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Actinobacillus succinogenes (strain ATCC 55618 / 130Z)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi44 – 441Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi47 – 471Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi51 – 511Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi79 – 791Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciASUC339671:GHDX-2119-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:Asuc_2038
OrganismiActinobacillus succinogenes (strain ATCC 55618 / 130Z)
Taxonomic identifieri339671 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus
ProteomesiUP000001114: Chromosome

Subcellular locationi

Periplasm UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Tat-type signalUniRule annotationAdd
BLAST
Chaini33 – 826794Periplasmic nitrate reductasePRO_5000259017Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi339671.Asuc_2038.

Structurei

3D structure databases

ProteinModelPortaliA6VQY7.
SMRiA6VQY7. Positions 35-825.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 93574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiHPKTRVA.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A6VQY7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MELNRRDFMK ANAAIAAAAA AGITIPVKNV QAADDGIRWD KAPCRYCGTG
60 70 80 90 100
CSVLVGTKDG RVVATQGDPD AEVNRGLNCI KGYFLSKIMY GADRVQQPLL
110 120 130 140 150
RMKDGKFDKE GDFTPISWEQ AFTIMAEKIK DILKKKEPNA IGMFSSGQTT
160 170 180 190 200
IYEGYAKVKL WKAGLRSNTI DPNARHCMAS AAVAFMRTFG MDEPMGCYND
210 220 230 240 250
IEKADTFVLW GSNMAEMHPI LWSRISDRRL SGDNVKVVVM STYEHRSFEL
260 270 280 290 300
ADTPIIFKPQ SDLAILNYIA NYIIQNDKVD WDFVNKHTKF KRGETNIGYG
310 320 330 340 350
LRPEHPLQQG TNAKTAGKMY DSDFEEFKKI VSEYTLDKAH EISGVPKDQL
360 370 380 390 400
ETLAKMYADP EQNLVSYWTM GFNQHTRGVW VNQMMYNVHL LTGKISKPGC
410 420 430 440 450
GPFSLTGQPS ACGTAREVGT FIHRLPADMV VTNPKHVAKA EEIWQLPAGT
460 470 480 490 500
IPTVPGFHAV AQSRALKDGK LNFLWQMCTN NMQGGPNINE ETFPGWRNPE
510 520 530 540 550
NFIVVSDPYP STSAVAADLI LPTCMWVEKE GGYGNAERRT QLWRQQVKGP
560 570 580 590 600
GESRSDLWQI VEFSKYFKTD EVWSEELLAQ KPEYRGKTLY EVLYKNGEVD
610 620 630 640 650
KFQVPTNIPG YINDEADHFG FYLQKGLFEE YASFGRGHGH DLAPFDVYHQ
660 670 680 690 700
VRGLRWPVVD GKETLWRYRE GYDPYVKSGE EVAFYGNPDN KAIILGVPYE
710 720 730 740 750
APAESPDEEY DLWLCTGRVL EHWHTGTMTR RVPELHKAFP NNLVWMHPTD
760 770 780 790 800
AKKRGLRHGD KVKLITRRGE MISHLDTRGR NKTPEGLIFT TFFDAGQLTN
810 820
KLTLDATDPI SFETDYKKCA VKVVKA
Length:826
Mass (Da):93,338
Last modified:August 21, 2007 - v1
Checksum:i26BCEDE3801039D8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000746 Genomic DNA. Translation: ABR75384.1.
RefSeqiWP_012073760.1. NC_009655.1.
YP_001345319.1. NC_009655.1.

Genome annotation databases

EnsemblBacteriaiABR75384; ABR75384; Asuc_2038.
GeneIDi5349259.
KEGGiasu:Asuc_2038.
PATRICi20762550. VBIActSuc117883_2139.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000746 Genomic DNA. Translation: ABR75384.1 .
RefSeqi WP_012073760.1. NC_009655.1.
YP_001345319.1. NC_009655.1.

3D structure databases

ProteinModelPortali A6VQY7.
SMRi A6VQY7. Positions 35-825.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 339671.Asuc_2038.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABR75384 ; ABR75384 ; Asuc_2038 .
GeneIDi 5349259.
KEGGi asu:Asuc_2038.
PATRICi 20762550. VBIActSuc117883_2139.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi HPKTRVA.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci ASUC339671:GHDX-2119-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A genomic perspective on the potential of Actinobacillus succinogenes for industrial succinate production."
    McKinlay J.B., Laivenieks M., Schindler B.D., McKinlay A.A., Siddaramappa S., Challacombe J.F., Lowry S.R., Clum A., Lapidus A.L., Burkhart K.B., Harkins V., Vieille C.
    BMC Genomics 11:680-680(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 55618 / 130Z.

Entry informationi

Entry nameiNAPA_ACTSZ
AccessioniPrimary (citable) accession number: A6VQY7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: August 21, 2007
Last modified: November 26, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3