Reviewed, UniProtKB/Swiss-Prot A6VQN1 (DDL_ACTSZ)

Names and origin

Protein names

Recommended name:
    D-alanine--D-alanine ligase
    EC=6.3.2.4
Alternative name(s):
    D-alanylalanine synthetase
    D-Ala-D-Ala ligase

Gene names

Name:	ddl
Ordered Locus Names:	Asuc_1930

Organism

Actinobacillus succinogenes (strain ATCC 55618 / 130Z) [Complete proteome] [HAMAP]

Taxonomic identifier

339671 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the D-alanine--D-alanine ligase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 307	307	D-alanine--D-alanine ligase		PRO_1000071100
Regions
	Domain	105 – 304	200	ATP-grasp
	Nucleotide binding	135 – 190	56	ATP By similarity
Sites
	Metal binding	258	1	Magnesium or manganese 1 By similarity
	Metal binding	271	1	Magnesium or manganese 1 By similarity
	Metal binding	271	1	Magnesium or manganese 2 By similarity
	Metal binding	273	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6VQN1-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 2FD6EFD953622A42	FASTA	307	33,644
	10 20 30 40 50 60 MKSLKQQKIA VLLGGTASER EVSLNSGAAV LDALRSQGYD AHPVDPKDMP VADLKQQGFE 70 80 90 100 110 120 RVFNILHGRG GEDGVIQGVL EQIGLPYTGC GVMTSALTMD KMRTKMLWKG FGLPIADMEI 130 140 150 160 170 180 VTKNTALSLE PQAIVARLGL PLMVKPSREG SSVGLTKVDS ADKLKSAVDL ALKFDDIVLI 190 200 210 220 230 240 EEWLSGDELT VPVLGDEVLP AVQIVPEGEF YDYNAKYISD NTRYICPMPM SDERWDELKN 250 260 270 280 290 300 LVKRAYEAVG CRGWSRIDVM TDSEGNFRLI EVNTTPGMTS HSLFPKSAAT VGYSFEKLVE KILELSV

References

[1]

"Complete sequence of Actinobacillus succinogenes 130Z." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Lowry S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Vieille C., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000746 Genomic DNA. Translation: ABR75278.1.
RefSeq	YP_001345213.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5348933.
GenomeReviews	Gene locus Asuc_1930 in contig CP000746_GR.
KEGG	asu:Asuc_1930.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00047. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013816. ATP_grasp_subdomain_2. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 2 hits. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DDL_ACTSZ

Accession

Primary (citable) accession number: A6VQN1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	August 21, 2007
Last modified:	November 25, 2008
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)