Reviewed, UniProtKB/Swiss-Prot A6VQD5 (SYM_ACTSZ)

Names and origin

Protein names

Recommended name:
    Methionyl-tRNA synthetase
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	Asuc_1831

Organism

Actinobacillus succinogenes (strain ATCC 55618 / 130Z) [Complete proteome] [HAMAP]

Taxonomic identifier

339671 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	685 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity.
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 685	685	Methionyl-tRNA synthetase HAMAP MF_00098		PRO_0000331774
Regions
	Domain	583 – 685	103	tRNA-binding
	Motif	15 – 25	11	"HIGH" region HAMAP MF_00098
	Motif	331 – 335	5	"KMSKS" region HAMAP MF_00098
Sites
	Metal binding	146	1	Zinc By similarity
	Metal binding	149	1	Zinc By similarity
	Metal binding	159	1	Zinc By similarity
	Metal binding	162	1	Zinc By similarity
	Binding site	334	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6VQD5-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: B9A51FBC266A3FBA	FASTA	685	77,776
	10 20 30 40 50 60 MSKANRQILV TCALPYANGP IHLGHMLEHI QADIWVRFQR MRGHEIYFVC ADDAHGTPIM 70 80 90 100 110 120 LKADQMGIAP EQLIADVQKS HYADFCGFNI SFDNYHSTHS EENREFSEMI YNRLKENGFI 130 140 150 160 170 180 KTRAISQLFD PEKSMFLPDR FVKGTCPKCK AEDQYGDNCE VCSATYSPTE LIAPRSVVSG 190 200 210 220 230 240 ATPIIKESEH FFFDLPSFEA MLKQWNRSGA LQSEVANKMQ EWFDAGLQQW DISRDAPYFG 250 260 270 280 290 300 FKIPGTENKY FYVWLDAPIG YMASFKNLCN RQNIDFDRFW NKDSQAELYH FIGKDIMYFH 310 320 330 340 350 360 SLFWPAMLEG ADLRKPSNIF VHGYVTVNGE KMSKSRGTFI QAATYLKHLD PECLRYYYAA 370 380 390 400 410 420 KLSNRIDDLD LNLEDFVQRV NTDLVNKLVN LASRNAGFIQ KRFAGKLADK LDDEALFNEF 430 440 450 460 470 480 IAQSAQIAAY YENREFGKAV REIMALTDKA NKYVDEKAPW VIAKEQGRET ELQRVCSMGI 490 500 510 520 530 540 QLFRVLMGYL KPVLPKLAER SEAFLQAELT WDNLTQPLLD HEIAPFKALF SRVDSKQIEA 550 560 570 580 590 600 MIEASKAENA AVNSSPKAEK TAKKTTALES EFEPLEAEIS IDDFAKMDLR VAKVISCEAV 610 620 630 640 650 660 PESKKLLKFQ LDLGFETRQV LSGIKAAYGN PEELMGRFVI MVANLAPRKM KFGMSEGMIL 670 680 SAGSGGEDLF LLDVDAGVKA GSRVK

References

[1]

"Complete sequence of Actinobacillus succinogenes 130Z." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Lowry S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Vieille C., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000746 Genomic DNA. Translation: ABR75182.1.
RefSeq	YP_001345117.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6VQD5.
Genome annotation databases
GeneID	5348429.
GenomeReviews	Gene locus Asuc_1831 in contig CP000746_GR.
KEGG	asu:Asuc_1831.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	CEAVPES.
Family and domain databases
HAMAP	MF_00098. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR002304. Met-tRNA-synth_Ia. IPR004495. Met-tRNA-synth_Ia_bsu_C. IPR012340. NA-bd_OB-fold. IPR014729. Rossmann-like_a/b/a_fold. IPR014758. tRNA-synt_met_N. IPR002547. tRNA_bd. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
Pfam	PF09334. tRNA-synt_1g. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit. TIGR00399. metG_C_term. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_ACTSZ

Accession

Primary (citable) accession number: A6VQD5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	August 21, 2007
Last modified:	November 3, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)