Reviewed, UniProtKB/Swiss-Prot A6VQD4 (PURA_ACTSZ)

Names and origin

Protein names

Recommended name:
    Adenylosuccinate synthetase
    EC=6.3.4.4
Alternative name(s):
    IMP--aspartate ligase
    AdSS
    AMPSase

Gene names

Name:	purA
Ordered Locus Names:	Asuc_1830

Organism

Actinobacillus succinogenes (strain ATCC 55618 / 130Z) [Complete proteome] [HAMAP]

Taxonomic identifier

339671 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays an important role in the de novo pathway of purine nucleotide biosynthesis. HAMAP MF_00011
Catalytic activity	GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. HAMAP MF_00011
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. HAMAP MF_00011
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the adenylosuccinate synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: HAMAP adenylosuccinate synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 432	432	Adenylosuccinate synthetase HAMAP MF_00011		PRO_1000070937
Regions
	Nucleotide binding	13 – 19	7	GTP Potential
Sites
	Active site	141	1	By similarity
	Active site	148	1	By similarity
	Metal binding	14	1	Magnesium By similarity
	Metal binding	41	1	Magnesium; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6VQD4-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 3414EE7F50702A0D	FASTA	432	47,047
	10 20 30 40 50 60 MGKSVVVLGA QWGDEGKGKI VDLLTDRVKY VVRYQGGHNA GHTLIINGEK TVLRLIPSGI 70 80 90 100 110 120 LHNNVTCLIG NGVVLSPSAL MQEMGELESR GINVRERLLI SEACPLIMPY HVAMDHAREA 130 140 150 160 170 180 ALGNNKIGTT GRGIGPAYED KVARRGLRVS DLFNKEKFAA KLKNVLDYYN FQLVQYYKAE 190 200 210 220 230 240 PVDYQKTLDD VMAIADIILG MVADISTILD TARKNGDNIL FEGAQGAMLD IDHGTYPFVT 250 260 270 280 290 300 SSNTTAGGVA TGAGFGPRNI DYVLGIIKAY CTRVGGGPFT TELFDETGAE IARKGNEFGA 310 320 330 340 350 360 VTGRPRRCGW FDAVAIRRAI QVNSISGFCM TKLDVLDGFD EVKICVGYKL PSGEVVDYAP 370 380 390 400 410 420 LSAKDWEGVE PVYESMPGWK ENTFGVTDYN KLPAAVHNYV KRIEQVTGVP VAILSTGPDR 430 VETMILQDPF KQ

References

[1]

"Complete sequence of Actinobacillus succinogenes 130Z." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Lowry S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Vieille C., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000746 Genomic DNA. Translation: ABR75181.1.
RefSeq	YP_001345116.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6VQD4.
Genome annotation databases
GeneID	5348428.
GenomeReviews	Gene locus Asuc_1830 in contig CP000746_GR.
KEGG	asu:Asuc_1830.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	YVLGIIK.
Family and domain databases
HAMAP	MF_00011. [Tree]
InterPro	IPR018220. Adenylosuccinate_synthase_AS. IPR001114. Adenylosuccinate_synthetase. [Graphical view]
PANTHER	PTHR11846. Asucc_synthtase. 1 hit.
Pfam	PF00709. Adenylsucc_synt. 1 hit. [Graphical view]
ProDom	PD001188. Asucc_synthtase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00788. Adenylsucc_synt. 1 hit. [Graphical view]
TIGRFAMs	TIGR00184. purA. 1 hit.
PROSITE	PS01266. ADENYLOSUCCIN_SYN_1. 1 hit. PS00513. ADENYLOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURA_ACTSZ

Accession

Primary (citable) accession number: A6VQD4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	August 21, 2007
Last modified:	November 3, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)