A6VQ79 (SYC_ACTSZ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine--tRNA ligase
EC=6.1.1.16

Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	Asuc_1774

Organism

Actinobacillus succinogenes (strain ATCC 55618 / 130Z) [Complete proteome] [HAMAP]

Taxonomic identifier

339671 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	459 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00041
Subunit structure	Monomer By similarity. HAMAP MF_00041
Subcellular location	Cytoplasm HAMAP MF_00041.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cysteine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 459	459	Cysteine--tRNA ligase HAMAP MF_00041		PRO_0000332782
Regions
	Motif	30 – 40	11	"HIGH" region HAMAP MF_00041
	Motif	266 – 270	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	28	1	Zinc By similarity
	Metal binding	209	1	Zinc By similarity
	Metal binding	234	1	Zinc By similarity
	Metal binding	238	1	Zinc By similarity
	Binding site	269	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6VQ79 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: F407FB9E55ADF824	FASTA	459	52,162
	10 20 30 40 50 60 MLKIFNTLTR EKEEFKPINQ NQVGMYVCGV TVYDLCHFGH GRTFVSFDVI TRYLRFLGYN 70 80 90 100 110 120 LRYVRNITDV DDKIIKRALE NNETCDQLVE RMIAEMHKDF DALNILRPDA EPRATKHIPE 130 140 150 160 170 180 IIAMVDALLQ RGHAYLAADG DVMFDVESFQ KYGALSRQNL EQLQAGARVE IKSVKKNPMD 190 200 210 220 230 240 FVLWKMSKPN EPSWDSPWGK GRPGWHIECS AMNSKELGNH FDIHGGGSDL MFPHHENEIA 250 260 270 280 290 300 QSCCAHDGEY VNYWLHTGML TINEEKMSKS LNNFFTIRDI LTKYDAESVR YFFLTAQYRS 310 320 330 340 350 360 LLDYSEENIG LARKALERLY TALRGCDWNS ELPAQDQYVG AFKDAMDDDF NTPGALAVLF 370 380 390 400 410 420 ELAREINKLK AENPAQANHL AARLKHLAGV LGLLEQDPEA FLQGGAENEE VGKIEALIKQ 430 440 450 RNDARAEKNW AAADAARDAL KEMGVVLEDG ANGTSWRKL

References

[1]

"Complete sequence of Actinobacillus succinogenes 130Z." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Lowry S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Vieille C., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 55618 / 130Z.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000746 Genomic DNA. Translation: ABR75126.1.
RefSeq	YP_001345061.1. NC_009655.1.
3D structure databases
ProteinModelPortal	A6VQ79.
SMR	A6VQ79. Positions 1-458.
ModBase	Search...
Protein-protein interaction databases
STRING	A6VQ79.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5348246.
GenomeReviews	Gene locus Asuc_1774 in contig CP000746_GR.
KEGG	asu:Asuc_1774.
PATRIC	20761966. VBIActSuc117883_1854.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0215.
HOGENOM	HBG327651.
OMA	DFDALNM.
ProtClustDB	PRK00260.
Family and domain databases
HAMAP	MF_00041. Cys_tRNA_synth. [Tree]
InterPro	IPR015803. Cys-tRNA-synt. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR024909. Cys-tRNA/MSH_ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01883.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SMART	SM00840. DALR_2. 1 hit. [Graphical view]
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00435. CysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_ACTSZ

Accession

Primary (citable) accession number: A6VQ79

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	August 21, 2007
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program