A6VQ68 (PLSB_ACTSZ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol-3-phosphate acyltransferase
Short name=GPAT
EC=2.3.1.15

Gene names

Name:	plsB
Ordered Locus Names:	Asuc_1763

Organism

Actinobacillus succinogenes (strain ATCC 55618 / 130Z) [Complete proteome] [HAMAP]

Taxonomic identifier

339671 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	813 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. HAMAP MF_00393
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. HAMAP MF_00393
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP MF_00393.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity. HAMAP MF_00393
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 813	813	Glycerol-3-phosphate acyltransferase HAMAP MF_00393		PRO_1000072227
Regions
	Motif	304 – 309	6	HXXXXD motif HAMAP MF_00393

Sequences

	Sequence		Length	Mass (Da)
	A6VQ68 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 77EAD4D86CCBB0C6	FASTA	813	93,109
	10 20 30 40 50 60 MSAFLNLYRN TLSWPLSFLV KDNPIPNNPV EELTLNIEQP IVYVLPYTSQ TDLVVLRKNC 70 80 90 100 110 120 LSLGLPDPFL DNRIEGKVLP RYVFLDEGHQ FFKSKGAKKE TEKVFKNYLE LHRTSADLDV 130 140 150 160 170 180 QVVPVSVLWG RSPGREDKGL PKLRLLNGLQ KTIAAIWFGR DTFVRFSQAM SMRYMVNEYG 190 200 210 220 230 240 EDEKLAQKLP RIAKMHFAKQ RISATGPRLP NRQAMFNKLL QHPAVLQAIE DEARGKNMTK 250 260 270 280 290 300 EKARKEAEKI LNEIAADTNY SSLRVADRLL GWLWNKLYQG IEVEHADRVR RLALEGHEIV 310 320 330 340 350 360 YVPCHRSHID YLLLSYVLYH QGLVPPHIAA GINLNFWPVG RIFRSWGAFF IRRTFKGNRL 370 380 390 400 410 420 YSTLFREYLG ELFHRGYSVE YFIEGGRSRT GRLLTPKTGM MSMTLQALQQ GQTRPISIVP 430 440 450 460 470 480 VYVGYEHVLE VDTYAKELRG AAKEKENAGL VLRVIKKLRN LGRGFVNFGE PITLSTYLNR 490 500 510 520 530 540 HFPEWKNEGR DERPLWFNKA VDAVSRQVMV NINKAAAVNA MNLTGTALLS SRQRALSREQ 550 560 570 580 590 600 LLEQLESYQQ YLLNVSYSDD IIIPSAPPKE LLDHVLGLDR VGILIEKDNF GELVRLERNH 610 620 630 640 650 660 AVLMTYYRNN IQHLLVLPSL VASIVLHHEA IQKDLVLTSV EKLYPFLKAE LFMHLPPEAL 670 680 690 700 710 720 REKVERIIAE LHRQQLIKLN ENILSINRPR VRTLQLWSAG MREILQRYLI TVAILLQDPA 730 740 750 760 770 780 ISRGKLEKES QSIAQRLSVL HGINSPEFFD KAVFSTFIAS LKDNGYFDTS GNADVTKLQG 790 800 810 MADILEHVIS TEINLTIKSA VETAVDLDEI ESE

References

[1]

"Complete sequence of Actinobacillus succinogenes 130Z." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Lowry S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Vieille C., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 55618 / 130Z.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000746 Genomic DNA. Translation: ABR75115.1.
RefSeq	YP_001345050.1. NC_009655.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6VQ68.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5347706.
GenomeReviews	Gene locus Asuc_1763 in contig CP000746_GR.
KEGG	asu:Asuc_1763.
PATRIC	20761940. VBIActSuc117883_1841.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2937.
HOGENOM	HBG296590.
OMA	WNKLYQG.
ProtClustDB	PRK04974.
Family and domain databases
HAMAP	MF_00393. Glyc3P_acyltrans. [Tree]
InterPro	IPR002123. Acyltransferase. IPR022284. G3P_O-AcylTrfase. [Graphical view]
KO	K00631.
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
PIRSF	PIRSF000437. GPAT_DHAPAT. 1 hit.
SMART	SM00563. PlsC. 1 hit. [Graphical view]
TIGRFAMs	TIGR03703. PlsB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLSB_ACTSZ

Accession

Primary (citable) accession number: A6VQ68

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	August 21, 2007
Last modified:	December 14, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program