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Protein

DNA topoisomerase 3

Gene

topB

Organism
Actinobacillus succinogenes (strain ATCC 55618 / 130Z)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi7Magnesium 1; catalyticUniRule annotation1
Metal bindingi103Magnesium 1; catalyticUniRule annotation1
Metal bindingi103Magnesium 2UniRule annotation1
Metal bindingi105Magnesium 2UniRule annotation1
Active sitei331O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-bindingUniRule annotation, Isomerase, TopoisomeraseUniRule annotation
LigandMagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

BioCyciASUC339671:G1G9D-1786-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 3UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IIIUniRule annotation
Gene namesi
Name:topBUniRule annotation
Ordered Locus Names:Asuc_1706Imported
OrganismiActinobacillus succinogenes (strain ATCC 55618 / 130Z)Imported
Taxonomic identifieri339671 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus
Proteomesi
  • UP000001114 Componenti: Chromosome

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei61Interaction with DNAUniRule annotation1
Sitei170Interaction with DNAUniRule annotation1
Sitei178Interaction with DNAUniRule annotation1
Sitei185Interaction with DNAUniRule annotation1
Sitei333Interaction with DNAUniRule annotation1

Protein-protein interaction databases

STRINGi339671.Asuc_1706

Structurei

3D structure databases

ProteinModelPortaliA6VQ11
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 134ToprimInterPro annotationAdd BLAST134

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni194 – 199Interaction with DNAUniRule annotation6

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108IYH Bacteria
COG0550 LUCA
HOGENOMiHOG000086848
KOiK03169
OMAiTYPRVDT
OrthoDBiPOG091H028X

Family and domain databases

CDDicd00186 TOP1Ac, 1 hit
cd03362 TOPRIM_TopoIA_TopoIII, 1 hit
Gene3Di1.10.290.10, 1 hit
1.10.460.10, 2 hits
2.70.20.10, 2 hits
HAMAPiMF_00953 Topoisom_3_prok, 1 hit
InterProiView protein in InterPro
IPR000380 Topo_IA
IPR003601 Topo_IA_2
IPR023406 Topo_IA_AS
IPR013497 Topo_IA_cen
IPR013824 Topo_IA_cen_sub1
IPR013825 Topo_IA_cen_sub2
IPR013826 Topo_IA_cen_sub3
IPR023405 Topo_IA_core_domain
IPR003602 Topo_IA_DNA-bd_dom
IPR005738 TopoIII
IPR006171 TOPRIM_domain
IPR034144 TOPRIM_TopoIII
PANTHERiPTHR11390 PTHR11390, 1 hit
PfamiView protein in Pfam
PF01131 Topoisom_bac, 1 hit
PF01751 Toprim, 1 hit
PRINTSiPR00417 PRTPISMRASEI
SMARTiView protein in SMART
SM00437 TOP1Ac, 1 hit
SM00436 TOP1Bc, 1 hit
SM00493 TOPRIM, 1 hit
SUPFAMiSSF56712 SSF56712, 1 hit
TIGRFAMsiTIGR01056 topB, 1 hit
PROSITEiView protein in PROSITE
PS00396 TOPOISOMERASE_I_PROK, 1 hit
PS50880 TOPRIM, 1 hit

Sequencei

Sequence statusi: Complete.

A6VQ11-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLFVAEKPS LARAIADVLP KPHSRGDGFI RCGENDYVTW CVGHLLEQAE
60 70 80 90 100
PDAYNPSYKQ WRLEHLPIVP QKWQLIPRKE VAKQLKIVEK LIHQADELVN
110 120 130 140 150
AGDPDREGQL LVDEVFSYAD LPADKKDKIQ RCLVSDLNPN AVEKAVNKLQ
160 170 180 190 200
PNRNFIPLAT SALARARADW LYGINMTRAY TIRGRQAGYN GVLSVGRVQT
210 220 230 240 250
PVLGLIVRRD LEIENFRPKD FFEVLAQIKS EQNPTALSFT ALWQPSKACE
260 270 280 290 300
DYQDEDGRVL SRALAENVIK RITRQPAEVT AYEDKREKQT APLPYSLSAL
310 320 330 340 350
QIDAAKRFAL SAQDVLDTCQ SLYEKHKLIT YPRSDCRYLP NEHFADRHAV
360 370 380 390 400
MNAISVHCTA YQTLPSAVNS EQKNRCWNDK KVEAHHAIIP TAKNRAVSLT
410 420 430 440 450
AREQDIYGLI ARQYLMQFCP DAEYRKSKIT LNIAGGTFIA QARNLQIAGW
460 470 480 490 500
KELIGKEDDD EQLEAPLPVV KKGQMLFCEK GEIVSKKTQP PKPFTDATLL
510 520 530 540 550
SAMTGIARFV QDKELKKILR ETDGLGTEAT RAGIIELLFK RGFLYKKGRN
560 570 580 590 600
IHSTEAGRIL IQALPDLATQ PDMTAQWEAQ LNGISQKETS YQSFMFQLTQ
610 620 630 640
MLPELVQFVN FSALRRLSAV ADRPKPTFKR KARKTADKSA VKSV
Length:644
Mass (Da):72,813
Last modified:August 21, 2007 - v1
Checksum:i755948186E1325AC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000746 Genomic DNA Translation: ABR75058.1
RefSeqiWP_012073435.1, NC_009655.1

Genome annotation databases

EnsemblBacteriaiABR75058; ABR75058; Asuc_1706
KEGGiasu:Asuc_1706

Similar proteinsi

Entry informationi

Entry nameiA6VQ11_ACTSZ
AccessioniPrimary (citable) accession number: A6VQ11
Entry historyiIntegrated into UniProtKB/TrEMBL: August 21, 2007
Last sequence update: August 21, 2007
Last modified: February 28, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
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Main funding by: National Institutes of Health