Reviewed, UniProtKB/Swiss-Prot A6VPZ1 (CYSI_ACTSZ)

Names and origin

Protein names

Recommended name:
    Sulfite reductase [NADPH] hemoprotein beta-component
      Short name=SIR-HP
      Short name=SIRHP
    EC=1.8.1.2

Gene names

Name:	cysI
Ordered Locus Names:	Asuc_1686

Organism

Actinobacillus succinogenes (strain ATCC 55618 / 130Z) [Complete proteome] [HAMAP]

Taxonomic identifier

339671 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	588 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate By similarity.
Catalytic activity	H(2)S + 3 NADP(+) + 3 H(2)O = sulfite + 3 NADPH.
Cofactor	Binds 1 siroheme per subunit By similarity. Binds 1 4Fe-4S cluster per subunit By similarity.
Subunit structure	Alpha(8)-beta(4). The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity.
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation Inferred from electronic annotation. Source: HAMAP
Cellular component	sulfite reductase complex (NADPH) Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW sulfite reductase (NADPH) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 588	588	Sulfite reductase [NADPH] hemoprotein beta-component		PRO_0000318561
Sites
	Metal binding	443	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	449	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	488	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	492	1	Iron (siroheme axial ligand) By similarity
	Metal binding	492	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6VPZ1-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: CA199CDA15D40BF1	FASTA	588	66,565
	10 20 30 40 50 60 MTDSTKQKGL EWQKQGLSDN ERLKAESNFL RGTILDDLED GLTGGFKGDN FQMIRFHGMY 70 80 90 100 110 120 EQDDRDIRAE RADEKLEARK FMLLRCRLPG GIIKPAQWIE IDKFARDNNY YQSIRLTNRQ 130 140 150 160 170 180 TFQYHGVPKT KLQEMHRLLH KLGLDSIATA SDMNRNVLCS SNPVESELHQ EAYEWAKKIS 190 200 210 220 230 240 EHLLPRTNGY LDVWIAGKKV QSSDSFLGQE DEPILGKTYL PRKYKTAVVL PPLNDVDMYG 250 260 270 280 290 300 NDMNFVGIQD EAGKLVGFNV LVGGGLSFEH GNTKTYPNVA LELGYIPVEQ TLKAAECIVT 310 320 330 340 350 360 TQRDFGNRAD RKNARLRYTL QNMTLDGFRE EVERRMGFKF ESIRPFEFTE RGDRIGWVKG 370 380 390 400 410 420 IDDKWHLTCF IESGRITDKP GKPLMTGMLE LAKVHTGDFR ITANQNIIIA NVAEEDKQRI 430 440 450 460 470 480 EDIAREYGLI GNISKLRENS MSCVSFPTCP LAMAESERAL PEFIDELDNI MAKHDVADDY 490 500 510 520 530 540 IVTRITGCPN GCGRAMLAEI GLVGKAIGRY NLHLGGDRPG TRIPRMYKEN ITLPEILAEL 550 560 570 580 DGLIGRWAKE RNSNEGFGDF VIRAGIIKPV VNAVVDFWDA NLIPTVTA

References

[1]

"Complete sequence of Actinobacillus succinogenes 130Z." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Lowry S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Vieille C., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000746 Genomic DNA. Translation: ABR75038.1.
RefSeq	YP_001344973.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5349238.
GenomeReviews	Gene locus Asuc_1686 in contig CP000746_GR.
KEGG	asu:Asuc_1686.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01540. [Tree]
InterPro	IPR011786. CysI. IPR006066. Nir_Si_BS. IPR006067. Nir_Sir_4Fe4S. IPR005117. NiRdtase/SiRdtase_haem-b_fer. [Graphical view]
Pfam	PF01077. NIR_SIR. 1 hit. PF03460. NIR_SIR_ferr. 2 hits. [Graphical view]
PRINTS	PR00397. SIROHAEM.
TIGRFAMs	TIGR02041. CysI. 1 hit.
PROSITE	PS00365. NIR_SIR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSI_ACTSZ

Accession

Primary (citable) accession number: A6VPZ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	August 21, 2007
Last modified:	November 25, 2008
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)