Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Class B acid phosphatase

Gene

aphA

Organism
Actinobacillus succinogenes (strain ATCC 55618 / 130Z)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Dephosphorylates several organic phosphate monoesters. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds (By similarity).By similarity

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.By similarity

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei68 – 681NucleophileBy similarity
Metal bindingi68 – 681MagnesiumBy similarity
Active sitei70 – 701Proton donorBy similarity
Metal bindingi70 – 701Magnesium; via carbonyl oxygenBy similarity
Binding sitei183 – 1831SubstrateBy similarity
Metal bindingi198 – 1981MagnesiumBy similarity

GO - Molecular functioni

  1. acid phosphatase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciASUC339671:GHDX-1478-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Class B acid phosphataseBy similarity (EC:3.1.3.2By similarityImported)
Short name:
CBAPBy similarity
Gene namesi
Name:aphABy similarity
Ordered Locus Names:Asuc_1410
OrganismiActinobacillus succinogenes (strain ATCC 55618 / 130Z)
Taxonomic identifieri339671 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus
ProteomesiUP000001114 Componenti: Chromosome

Subcellular locationi

Periplasm By similarity

GO - Cellular componenti

  1. outer membrane-bounded periplasmic space Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 243221Class B acid phosphataseSequence AnalysisPRO_5000258893Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi339671.Asuc_1410.

Structurei

3D structure databases

ProteinModelPortaliA6VP72.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni136 – 1372Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class B bacterial acid phosphatase family.By similarity

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3700.
HOGENOMiHOG000270623.
KOiK03788.
OMAiYFITGRT.
OrthoDBiEOG6HTNVZ.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR005519. Acid_phosphat_B.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view]
PfamiPF03767. Acid_phosphat_B. 1 hit.
[Graphical view]
PIRSFiPIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01672. AphA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A6VP72-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLIKSSVFM FSMLTIAVSA NAKGPKVSYD HAGITAIDHK NEAQVKWVSA
60 70 80 90 100
ESIQKELKGK PPITVSFDID DTVLVSSQCF YYGKQTFSPD SLDYLHNQAY
110 120 130 140 150
WDFVADGCDN YSIPKESAKK LIDMHQARGD QVIFITGRTP HRLHKAGQMD
160 170 180 190 200
QLARILERAF HIKNMKPINY THDKPIAPYK YDKTRFMVEN RVSIHYGDSN
210 220 230 240
DDILAAREAG IRGIRVIRSA TSTNRPLPLN GGYGEEVVED SSY
Length:243
Mass (Da):27,365
Last modified:August 21, 2007 - v1
Checksum:i32E3AAF37288EB6F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000746 Genomic DNA. Translation: ABR74769.1.
RefSeqiYP_001344704.1. NC_009655.1.

Genome annotation databases

EnsemblBacteriaiABR74769; ABR74769; Asuc_1410.
KEGGiasu:Asuc_1410.
PATRICi20761194. VBIActSuc117883_1473.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000746 Genomic DNA. Translation: ABR74769.1.
RefSeqiYP_001344704.1. NC_009655.1.

3D structure databases

ProteinModelPortaliA6VP72.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi339671.Asuc_1410.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABR74769; ABR74769; Asuc_1410.
KEGGiasu:Asuc_1410.
PATRICi20761194. VBIActSuc117883_1473.

Phylogenomic databases

eggNOGiCOG3700.
HOGENOMiHOG000270623.
KOiK03788.
OMAiYFITGRT.
OrthoDBiEOG6HTNVZ.

Enzyme and pathway databases

BioCyciASUC339671:GHDX-1478-MONOMER.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR005519. Acid_phosphat_B.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view]
PfamiPF03767. Acid_phosphat_B. 1 hit.
[Graphical view]
PIRSFiPIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01672. AphA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "A genomic perspective on the potential of Actinobacillus succinogenes for industrial succinate production."
    McKinlay J.B., Laivenieks M., Schindler B.D., McKinlay A.A., Siddaramappa S., Challacombe J.F., Lowry S.R., Clum A., Lapidus A.L., Burkhart K.B., Harkins V., Vieille C.
    BMC Genomics 11:680-680(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 55618 / 130Z.

Entry informationi

Entry nameiAPHA_ACTSZ
AccessioniPrimary (citable) accession number: A6VP72
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: August 21, 2007
Last modified: April 1, 2015
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.