Reviewed, UniProtKB/Swiss-Prot A6VP48 (PYRD_ACTSZ)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase
    EC=1.3.3.1
Alternative name(s):
    Dihydroorotate oxidase
    DHOdehase
      Short name=DHODase
      Short name=DHOD

Gene names

Name:	pyrD
Ordered Locus Names:	Asuc_1385

Organism

Actinobacillus succinogenes (strain ATCC 55618 / 130Z) [Complete proteome] [HAMAP]

Taxonomic identifier

339671 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + O2 = orotate + H2O2. HAMAP MF_00225
Cofactor	Binds 1 FMN per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 4/6. HAMAP MF_00225
Subunit structure	Homodimer By similarity.
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cell membrane Membrane
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	Dihydroorotate dehydrogenase HAMAP MF_00225		PRO_1000071764
Sites
	Active site	178	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6VP48-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 887C5F00632C2B96	FASTA	340	37,055
	10 20 30 40 50 60 MLYPLIRKGI FALEPETAHN LAIKALHIAG NPWLNRALKA LLGCPQGTEK TVMGIKFKNP 70 80 90 100 110 120 IGLAAGADKN GEAIDGFGAM GFGFIEVGTV TPLAQDGNAK PRQFRIVEAE GIINRNGFNN 130 140 150 160 170 180 YGIDHLIENV NKSHYDGTLG INIGKNKITP IEQGKDDYIF CLNKAYNYAH YITVNISSPN 190 200 210 220 230 240 TPDLRTLQYG DALDDLLKSI KERQKILAEQ YNKYVPIALK IAPDLTEAEL VQIADTVRRH 250 260 270 280 290 300 QMDGVIATNT TISRDTVQGM KNAAEIGGLS GKPLQHKSTE IIRRLHRELK GDIPIIGSGG 310 320 330 340 IDGVQNAQEK IAAGAELLQI YSGLIYHGPA LVKTLVQTIK

References

[1]

"Complete sequence of Actinobacillus succinogenes 130Z." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Lowry S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Vieille C., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000746 Genomic DNA. Translation: ABR74745.1.
RefSeq	YP_001344680.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5348572.
GenomeReviews	Gene locus Asuc_1385 in contig CP000746_GR.
KEGG	asu:Asuc_1385.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A6VP48. AALNRMG.
Family and domain databases
HAMAP	MF_00225. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR012135. Dihydroorotate_DH_1_2. IPR005719. Dihydroorotate_DH_2. IPR001295. Dihydroorotate_DH_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMs	TIGR01036. pyrD_sub2. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRD_ACTSZ

Accession

Primary (citable) accession number: A6VP48

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	August 21, 2007
Last modified:	June 16, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)