Reviewed, UniProtKB/Swiss-Prot A6VP15 (GLGB_ACTSZ)

Names and origin

Protein names

Recommended name:
    1,4-alpha-glucan-branching enzyme
    EC=2.4.1.18
Alternative name(s):
    Glycogen-branching enzyme
      Short name=BE
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase

Gene names

Name:	glgB
Ordered Locus Names:	Asuc_1352

Organism

Actinobacillus succinogenes (strain ATCC 55618 / 130Z) [Complete proteome] [HAMAP]

Taxonomic identifier

339671 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	829 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity.
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 829	829	1,4-alpha-glucan-branching enzyme HAMAP MF_00685		PRO_1000072735
Sites
	Active site	300	1	By similarity
	Active site	335	1	By similarity
	Active site	340	1	By similarity
	Active site	403	1	By similarity
	Active site	405	1	By similarity
	Active site	458	1	By similarity
	Active site	525	1	By similarity
	Active site	526	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6VP15-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 5BE0A3BD25EFE7A5	FASTA	829	94,021
	10 20 30 40 50 60 MTTFVTQDTI NAFFDGRHAD PFAVLGMHET DNGIEIRALL PDAGRVVVID KETQDEVCEL 70 80 90 100 110 120 SRIDERGFFA GIMPQRNSFF NYQLQVSWGN EVLRIEDPYR FHPMIQELDN WLLAEGSHLR 130 140 150 160 170 180 PYEVLGAHFM ECDHVSGVNF RLWAPNAKRV SVVGDFNYWD GRRHPMRFHQ ASGVWELFIP 190 200 210 220 230 240 KVALGDLYKF ELLDNNNRLR LKSDPYAFAA QLRPDTASQI SVLPEIQEMT AARRKANQLD 250 260 270 280 290 300 QPISIYEVHL GSWRRNLANN FWLNYDEIAD ELIPYVKEMG FTHIELLPIS EFPFDGSWGY 310 320 330 340 350 360 QPIGLYAPTS RFGSPEGFKR FVDKAHAAGI NVILDWVPGH FPSDTHGLAT FDGTALYEHA 370 380 390 400 410 420 DPKEGYHQDW NTLIYNYGRH EVKNYLSGNA LYWVERFGLD GIRVDAVASM IYRDYSRRDG 430 440 450 460 470 480 EWIPNQYGGR ENLEAIEFLK HTNYILGTEH PGVMCVAEES TAFAGVTLPP ENGGLGFNFK 490 500 510 520 530 540 WNMGWMNDTL SYMKLDPIYR QYNHSKLTFG MLYQYSENFV LPLSHDEVVH GKCSLLDKMP 550 560 570 580 590 600 GDTWQKFANL RAYYGYMWAY PGKKLLFMGN EFAQGREWNY QESLDWYLLD EFHGGGWHSG 610 620 630 640 650 660 VQRLVKDLNK TYQKQSALYQ LDTKPEGFEW LVVDDAQNSV FVFERRNAKG EPLIVVSNFT 670 680 690 700 710 720 PVPRENYRFG VNVAGSYEEI LNTDADIYKG SGLNNGGVID SEEIESHGKT QSISITVPPL 730 740 750 760 770 780 ATVYFKLKSA RKVASPRKVT KKKTTADGAE ATAKNASASK ATKVSTKKTV KSSEAKPVKA 790 800 810 820 ATKSSVTKVA TKKSTDKPTA KATRTKKATV TKTAKASAKT TAKKTKDNA

References

[1]

"Complete sequence of Actinobacillus succinogenes 130Z." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Lowry S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Vieille C., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000746 Genomic DNA. Translation: ABR74712.1.
RefSeq	YP_001344647.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6VP15.
Genome annotation databases
GeneID	5349403.
GenomeReviews	Gene locus Asuc_1352 in contig CP000746_GR.
KEGG	asu:Asuc_1352.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	PKTASIV.
Family and domain databases
HAMAP	MF_00685. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz_core. IPR006048. A-amylase_b_C. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_ACTSZ

Accession

Primary (citable) accession number: A6VP15

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	August 21, 2007
Last modified:	November 3, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)