Reviewed, UniProtKB/Swiss-Prot A6VNZ2 (SYT_ACTSZ)

Names and origin

Protein names

Recommended name:
    Threonyl-tRNA synthetase
    EC=6.1.1.3
Alternative name(s):
    Threonine--tRNA ligase
      Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	Asuc_1329

Organism

Actinobacillus succinogenes (strain ATCC 55618 / 130Z) [Complete proteome] [HAMAP]

Taxonomic identifier

339671 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	643 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00184
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP threonine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 643	643	Threonyl-tRNA synthetase HAMAP MF_00184		PRO_1000071670
Regions
	Region	243 – 534	292	Catalytic HAMAP MF_00184
Sites
	Metal binding	334	1	Zinc; catalytic By similarity
	Metal binding	385	1	Zinc; catalytic By similarity
	Metal binding	511	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6VNZ2-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 4DA1A956F06681FB	FASTA	643	73,849
	10 20 30 40 50 60 MPIITLPDGS QRQFDNPVSV LEVAQSIGSG LAKATIAGRV NGERRDACDM INDDSTLEII 70 80 90 100 110 120 TAKDEDGLEI IRHSCAHLLG HAIKQLFPDV KMAIGPTIEN GFYYDVDLDR SLTQEDLDTL 130 140 150 160 170 180 EKRMSELAKT NYDVVKQRVT WQEARDTFEK RGEPYKMAIL DENIAKDDHP ALYHHQEYID 190 200 210 220 230 240 MCRGPHVPNM RFCHHFKLMK VAGAYWRGDS KNKMLQRIYG TAWADKKQLN GYLTRLEEAA 250 260 270 280 290 300 KRDHRKIGKA LDLYHMQEEA PGMVFWHNDG WTVFRELETF VRTKLKEYDY QEVKGPFMMD 310 320 330 340 350 360 RVLWEKTGHW QNYGDLMFTT QSENREYAIK PMNCPGHVQI FNQGLKSYRD LPIRMAEFGS 370 380 390 400 410 420 CHRNEPSGSL HGLMRVRGFT QDDAHIFCTE DQIESEVTSC IRMVYDIYGT FGFTDIAVKL 430 440 450 460 470 480 STRPEKRIGA DEMWDRAEQG LANALKHNAL EYEIQEGEGA FYGPKIEFAL RDCLDREWQC 490 500 510 520 530 540 GTIQLDFALP GRLNASYVAE DNDRRTPVMI HRAILGSIER FIGIITEEYA GFFPAWLAPV 550 560 570 580 590 600 QAVVMNITDS QAEYVQKVVK QLSDAGLRVK ADLRNEKVGF KVREHTLRRV PYMLVCGDKE 610 620 630 640 IAEGKVSVRT RRGTDLGTYD VEDFIEILKQ QVRGRELKLL GEG

References

[1]

"Complete sequence of Actinobacillus succinogenes 130Z." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Lowry S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Vieille C., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000746 Genomic DNA. Translation: ABR74689.1.
RefSeq	YP_001344624.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6VNZ2.
Genome annotation databases
GeneID	5349133.
GenomeReviews	Gene locus Asuc_1329 in contig CP000746_GR.
KEGG	asu:Asuc_1329.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	NTKIAIG.
Family and domain databases
HAMAP	MF_00184. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR012675. b-grasp_ferredoxin-like. IPR004095. TGS. IPR002320. Thr-tRNA-synth_IIa. IPR018158. Thr-tRNA-synth_IIa_cons-reg. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
TIGRFAMs	TIGR00418. thrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_ACTSZ

Accession

Primary (citable) accession number: A6VNZ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	August 21, 2007
Last modified:	November 3, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)