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A6VNF1

- BIOB_ACTSZ

UniProt

A6VNF1 - BIOB_ACTSZ

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Protein

Biotin synthase

Gene

bioB

Organism
Actinobacillus succinogenes (strain ATCC 55618 / 130Z)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi65 – 651Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi69 – 691Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi72 – 721Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi109 – 1091Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi140 – 1401Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi200 – 2001Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi272 – 2721Iron-sulfur 2 (2Fe-2S)UniRule annotation

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciASUC339671:GHDX-1197-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:Asuc_1132
OrganismiActinobacillus succinogenes (strain ATCC 55618 / 130Z)
Taxonomic identifieri339671 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus
ProteomesiUP000001114: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 338338Biotin synthasePRO_0000381183Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi339671.Asuc_1132.

Structurei

3D structure databases

ProteinModelPortaliA6VNF1.
SMRiA6VNF1. Positions 17-325.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiRIMMPAS.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

A6VNF1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
METTLQLYSN TPHPQAQYWS VCKVEALFET PFLELVHRAA IVHREHFNPQ
60 70 80 90 100
AVQLSTLMSI KTGGCPEDCG YCPQSARYRT GVQKQALLEV EDIVEKAKIA
110 120 130 140 150
KSRGASRFCM GAAWRGPKPK DIGKITEIVK AVKDLGLETC GTFGLLEDGM
160 170 180 190 200
AENLKEAGLD YYNHNIDTSP EHYKKVIGTR GFEDRLSTLG KVRKAGLKVC
210 220 230 240 250
CGGIIGMNEN RKERAGFIAS LANLDPQPES VPINQLVKVD GTPLADAEEL
260 270 280 290 300
DWTEFVRTIA VARITMPKSY VRLSAGRQGM SEEMQAMCFM AGANSIFYGD
310 320 330
KLLVTGNAEE DRDQLLMAKL DLEPETAENR RVSVQPER
Length:338
Mass (Da):37,460
Last modified:August 21, 2007 - v1
Checksum:iCAD4F34DBEABC126
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000746 Genomic DNA. Translation: ABR74498.1.
RefSeqiWP_012072875.1. NC_009655.1.
YP_001344433.1. NC_009655.1.

Genome annotation databases

EnsemblBacteriaiABR74498; ABR74498; Asuc_1132.
GeneIDi5348257.
KEGGiasu:Asuc_1132.
PATRICi20760644. VBIActSuc117883_1200.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000746 Genomic DNA. Translation: ABR74498.1 .
RefSeqi WP_012072875.1. NC_009655.1.
YP_001344433.1. NC_009655.1.

3D structure databases

ProteinModelPortali A6VNF1.
SMRi A6VNF1. Positions 17-325.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 339671.Asuc_1132.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABR74498 ; ABR74498 ; Asuc_1132 .
GeneIDi 5348257.
KEGGi asu:Asuc_1132.
PATRICi 20760644. VBIActSuc117883_1200.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi RIMMPAS.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci ASUC339671:GHDX-1197-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "A genomic perspective on the potential of Actinobacillus succinogenes for industrial succinate production."
    McKinlay J.B., Laivenieks M., Schindler B.D., McKinlay A.A., Siddaramappa S., Challacombe J.F., Lowry S.R., Clum A., Lapidus A.L., Burkhart K.B., Harkins V., Vieille C.
    BMC Genomics 11:680-680(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 55618 / 130Z.

Entry informationi

Entry nameiBIOB_ACTSZ
AccessioniPrimary (citable) accession number: A6VNF1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: August 21, 2007
Last modified: October 1, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3