Reviewed, UniProtKB/Swiss-Prot A6VMX3 (AMPA_ACTSZ)

Names and origin

Protein names

Recommended name:
    Probable cytosol aminopeptidase
    EC=3.4.11.1
Alternative name(s):
    Leucine aminopeptidase
      Short name=LAP
    Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	Asuc_0952

Organism

Actinobacillus succinogenes (strain ATCC 55618 / 130Z) [Complete proteome] [HAMAP]

Taxonomic identifier

339671 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	484 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP MF_00181
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181
Cofactor	Binds 2 manganese ions per subunit By similarity. HAMAP MF_00181
Subcellular location	Cytoplasm By similarity HAMAP MF_00181.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP metalloexopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 484	484	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_1000071652
Sites
	Active site	268	1	Potential
	Active site	342	1	Potential
	Metal binding	256	1	Manganese 2 By similarity
	Metal binding	261	1	Manganese 1 By similarity
	Metal binding	261	1	Manganese 2 By similarity
	Metal binding	279	1	Manganese 2 By similarity
	Metal binding	338	1	Manganese 1 By similarity
	Metal binding	340	1	Manganese 1 By similarity
	Metal binding	340	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6VMX3-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 76C761DFE2C44209	FASTA	484	52,324
	10 20 30 40 50 60 MKYSANLTAL LQTDTVLVAV FEDGSLSPSA QQFDGQGEVS ALLKSGDISG KIGEIATFRR 70 80 90 100 110 120 QNQRIIVVGA GKPNDINERQ FKQITQKAFQ AVKPTSAQTL ANALTEVQIN GRDLYWNIRF 130 140 150 160 170 180 GLETIAAESY IFDEFKSKKA DPVKLRNVIF HADDKQAQLA VQHGSAIALG VKLARDIANC 190 200 210 220 230 240 PPNVCNPNYL AAQAKKLEKQ TALLTTAVLG EKEMAELGMH AYLGVSQGSK NEAQLSVMTY 250 260 270 280 290 300 KNHPDPHAKP IVLVGKGLTF DAGGISLKPS ADMDEMKYDM CGAASIFGVM NALVELQLPL 310 320 330 340 350 360 NVIGVMAGCE NLPDGNAYRP GDILTTMNGL TVEVLNTDAE GRLVLCDTLT YVERFEPECV 370 380 390 400 410 420 VDVATLTGAC VVALGQHNSG LISTNDELAE QLFRASQQTQ DKAWRLPLSD EYQEQLKSPF 430 440 450 460 470 480 ADLANIGGRW GGAITAGAFL SNFTKQYRWA HLDIAGTAWL QGANKGATGR PVSLLTQFLI NQCQ

References

[1]

"Complete sequence of Actinobacillus succinogenes 130Z." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Lowry S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Vieille C., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000746 Genomic DNA. Translation: ABR74320.1.
RefSeq	YP_001344255.1.
3D structure databases
SMR	A6VMX3. Positions 1-483.
ModBase	Search...
Protein-protein interaction databases
STRING	A6VMX3.
Protein family/group databases
MEROPS	M17.003.
Genome annotation databases
GeneID	5348168.
GenomeReviews	Gene locus Asuc_0952 in contig CP000746_GR.
KEGG	asu:Asuc_0952.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0260.
HOGENOM	HBG742580.
OMA	LGHHNSG.
Family and domain databases
HAMAP	MF_00181. Cytosol_peptidase_M17. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR008283. Peptidase_M17_N. [Graphical view]
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_ACTSZ

Accession

Primary (citable) accession number: A6VMX3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	August 21, 2007
Last modified:	January 19, 2010
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)