Reviewed, UniProtKB/Swiss-Prot A6VMA0 (TYSY_ACTSZ)

Names and origin

Protein names

Recommended name:
    Thymidylate synthase
      Short name=TS
      Short name=TSase
    EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	Asuc_0725

Organism

Actinobacillus succinogenes (strain ATCC 55618 / 130Z) [Complete proteome] [HAMAP]

Taxonomic identifier

339671 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	283 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type thyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 283	283	Thymidylate synthase HAMAP MF_00008		PRO_1000070924
Sites
	Active site	160	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6VMA0-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: D42E84457CB75CC0	FASTA	283	32,542
	10 20 30 40 50 60 MRQYLDLCQR IVDEGVWLEN QRTGKRCLTV INADLTYNAA ANEFPIITTR KSYWKAAIAE 70 80 90 100 110 120 FLGYIRGYDN AADFRKLGAK TWDANANENQ AWLNNPVRKG VDDMGRVYGV QGRAWRKPNG 130 140 150 160 170 180 ETVDQLRKIV DDLSRGIDDR GEILTFLNPG EFDLGCLRPC MYSHTFSLLG DTLYLTSYQR 190 200 210 220 230 240 SCDVPLGLNF NQIQVFTFLA LMAQITGKKP GQAYHKIVNA HIYEDQLDLM RDVQLKRRPF 250 260 270 280 PSPRLEINPD IKTLEDLETW VTLDDFNVVG YECHEPIKYP FSV

References

[1]

"Complete sequence of Actinobacillus succinogenes 130Z." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Lowry S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Vieille C., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000746 Genomic DNA. Translation: ABR74097.1.
RefSeq	YP_001344032.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6VMA0.
Genome annotation databases
GeneID	5349015.
GenomeReviews	Gene locus Asuc_0725 in contig CP000746_GR.
KEGG	asu:Asuc_0725.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	GRAWRKP.
Family and domain databases
HAMAP	MF_00008. [Tree]
InterPro	IPR000398. Thymidylat_synth_C. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
ProDom	PD001180. Thymidylat_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03284. thym_sym. 1 hit.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_ACTSZ

Accession

Primary (citable) accession number: A6VMA0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	August 21, 2007
Last modified:	November 3, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)