Reviewed, UniProtKB/Swiss-Prot A6VLU1 (ILVC_ACTSZ)

Names and origin

Protein names

Recommended name:
    Ketol-acid reductoisomerase
    EC=1.1.1.86
Alternative name(s):
    Acetohydroxy-acid isomeroreductase
    Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	Asuc_0563

Organism

Actinobacillus succinogenes (strain ATCC 55618 / 130Z) [Complete proteome] [HAMAP]

Taxonomic identifier

339671 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 493	493	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_1000072318
Sites
	Active site	132	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	A6VLU1-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 30705DD722BC06D3	FASTA	493	54,331
	10 20 30 40 50 60 MSNYFNTLNL REKLDQLGRC RFMDRSEFSD GCNFLKGKKI VIVGCGAQGL NQGLNMRDSG 70 80 90 100 110 120 LDISYALRKA AIEEKRPSFQ RATENGFKVG TYEELIPTAD LVINLTPDKQ HSKVVSDVMP 130 140 150 160 170 180 LMKQGAAFGY SHGFNIVEVG EQIREDITVV MSAPKCPGTE VREEYKRGFG VPTLIAVHPA 190 200 210 220 230 240 NDPKGEGMAI AKAWASATGG DRAGVLESSF VAEVKSDLMG EQTILCGMLQ AGSIVCYDKL 250 260 270 280 290 300 IADGKDPAYA GKLIQYGWET ITEALKQGGI TLMMDRLSNA AKLRAFELSE QIKEKLGFLY 310 320 330 340 350 360 LKHMDDIISG EFSRVMMEDW ANGDKDLLAW REATGKTAFE NAPKADGIKI SEQEYFDNGV 370 380 390 400 410 420 LMVAMVKAGV EMAFDAMVAS GIYEESAYYE SLHELPLIAN TIARKRLYEM NVVISDTAEY 430 440 450 460 470 480 GNYLFSNVAT PILAKEIIPA LQKGDLGEPT PAVDIDNVTL RDVNDEIRNH PVELIGLELR 490 GYMTDMKRIS SQG

References

[1]

"Complete sequence of Actinobacillus succinogenes 130Z." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Lowry S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Vieille C., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000746 Genomic DNA. Translation: ABR73938.1.
RefSeq	YP_001343873.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5348214.
GenomeReviews	Gene locus Asuc_0563 in contig CP000746_GR.
KEGG	asu:Asuc_0563.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A6VLU1. LKHGGIT.
Family and domain databases
HAMAP	MF_00435. [Tree]
InterPro	IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. IPR014359. KetolA_reductoisomerase_bac. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
PIRSF	PIRSF000116. IlvC_gammaproteo. 1 hit.
TIGRFAMs	TIGR00465. ilvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_ACTSZ

Accession

Primary (citable) accession number: A6VLU1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	August 21, 2007
Last modified:	June 16, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)