Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A6VLR7 (TRMB_ACTSZ) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:Asuc_0539
OrganismActinobacillus succinogenes (strain ATCC 55618 / 130Z) [Complete proteome] [HAMAP]
Taxonomic identifier339671 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus

Protein attributes

Sequence length252 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 252252tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_1000072994

Regions

Region231 – 2344Substrate binding By similarity

Sites

Active site1551 By similarity
Binding site801S-adenosyl-L-methionine By similarity
Binding site1051S-adenosyl-L-methionine By similarity
Binding site1321S-adenosyl-L-methionine By similarity
Binding site1551S-adenosyl-L-methionine By similarity
Binding site1591Substrate By similarity
Binding site1911Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
A6VLR7 [UniParc].

Last modified August 21, 2007. Version 1.
Checksum: 51E9BC580F50A723

FASTA25229,082
        10         20         30         40         50         60 
MTELKKTFAD QKRKTVETAE FTEDGRYKRK IRSFVLRTGR LSEYQRQMMN DNWSNYGLDY 

        70         80         90        100        110        120 
QTEPFDFPAI YGNHNPVILE IGFGMGKSLV EMAQQNPDKN YLGIEVHTPG VGACLAYALE 

       130        140        150        160        170        180 
KGIKNLRVIC HDATEILRDC IADRSLGGLQ LFFPDPWHKA KHHKRRIVQP QFVKTVTQKL 

       190        200        210        220        230        240 
TSGGFIHFAT DWQNYAEHML NVLQSVQSAV GIRNISETGD FIPRPDFRPL TKFEQRGQKL 

       250 
GHGIWDLYFI KN 

« Hide

References

[1]"Complete sequence of Actinobacillus succinogenes 130Z."
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Lowry S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Vieille C., Richardson P.
Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 55618 / 130Z.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000746 Genomic DNA. Translation: ABR73914.1.
RefSeqYP_001343849.1. NC_009655.1.

3D structure databases

ProteinModelPortalA6VLR7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING339671.Asuc_0539.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABR73914; ABR73914; Asuc_0539.
GeneID5348552.
KEGGasu:Asuc_0539.
PATRIC20759302. VBIActSuc117883_0571.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMAHSTLEMA.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycASUC339671:GHDX-561-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_ACTSZ
AccessionPrimary (citable) accession number: A6VLR7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: August 21, 2007
Last modified: June 11, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways