A6VLP6 (TRMD_ACTSZ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(1)-)-methyltransferase
EC=2.1.1.228

Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	Asuc_0518

Organism

Actinobacillus succinogenes (strain ATCC 55618 / 130Z) [Complete proteome] [HAMAP]

Taxonomic identifier

339671 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605
Catalytic activity	S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605
Subunit structure	Homodimer By similarity. HAMAP MF_00605
Subcellular location	Cytoplasm Potential HAMAP MF_00605.
Sequence similarities	Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_1000072635
Regions
	Region	133 – 138	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	113	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6VLP6 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 3509DDFBE26C8F52	FASTA	245	27,461
	10 20 30 40 50 60 MFIGIVTLFP EMFKAITEFG VTGRAVKQNL LQVRCYNPRD FTHDKHKTVD DRPYGGGPGM 70 80 90 100 110 120 LMMVQPLRDA IHSAKAEAGE GVKVIYLSPQ GRKLEQSGVT ELAQNKKLIL VCGRYEGIDE 130 140 150 160 170 180 RLIQTEVDEE WSIGDYVLTG GELPAMTLID AVARFIPGVL GKQASADEDS FAAGLLDCPH 190 200 210 220 230 240 YTRPEELDGL TVPPVLMSGN HEQIRKWRLT QSLERTWLRR PELLEKLALT DEQKKILNQI KSETV

References

[1]

"Complete sequence of Actinobacillus succinogenes 130Z." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Lowry S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Vieille C., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 55618 / 130Z.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000746 Genomic DNA. Translation: ABR73893.1.
RefSeq	YP_001343828.1. NC_009655.1.
3D structure databases
ProteinModelPortal	A6VLP6.
SMR	A6VLP6. Positions 1-243.
ModBase	Search...
Protein-protein interaction databases
STRING	A6VLP6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5349056.
GenomeReviews	Gene locus Asuc_0518 in contig CP000746_GR.
KEGG	asu:Asuc_0518.
PATRIC	20759260. VBIActSuc117883_0550.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0336.
HOGENOM	HBG285805.
OMA	VCGRFEG.
ProtClustDB	PRK00026.
Family and domain databases
HAMAP	MF_00605. TrmD. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. IPR023148. tRNA_m1G_MeTrfase_C. [Graphical view]
Gene3D	G3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
KO	K00554.
PANTHER	PTHR10056. PTHR10056. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMs	TIGR00088. TrmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_ACTSZ

Accession

Primary (citable) accession number: A6VLP6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	August 21, 2007
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program