Reviewed, UniProtKB/Swiss-Prot A6VLN1 (ASNA_ACTSZ)

Names and origin

Protein names

Recommended name:
    Aspartate--ammonia ligase
    EC=6.3.1.1
Alternative name(s):
    Asparagine synthetase A

Gene names

Name:	asnA
Ordered Locus Names:	Asuc_0503

Organism

Actinobacillus succinogenes (strain ATCC 55618 / 130Z) [Complete proteome] [HAMAP]

Taxonomic identifier

339671 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: HAMAP tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Aspartate--ammonia ligase HAMAP MF_00555		PRO_1000072559

Sequences

	Sequence		Length	Mass (Da)
	A6VLN1-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 2C1A482D2B9E58BE	FASTA	330	37,540
	10 20 30 40 50 60 MKKSFILQQQ EISFAKNTFT TKLAEHLGLV EVQGPILSQV GNGIQDNLSG KEKPVQVNVK 70 80 90 100 110 120 MIEDATFEVV HSLAKWKRHT LARFGFAEGE GLFVHMKALR PDEDSLDQTH SVYVDQWDWE 130 140 150 160 170 180 KVIPAGRRNL NYLKETVRSI YAAIVETQEA VEQKFGLKAF LPKEITFIHS EDLARDYPHM 190 200 210 220 230 240 TDKERENEIC KKYGAVFLIG IGGDLPDGKP HDMRAPDYDD WTTSSEGEYK GLNGDILVWN 250 260 270 280 290 300 PVLNRAFELS SMGIRVDETA LRRQLALTHD EERLNFAWHQ DLINGRMPLS IGGGIGQSRL 310 320 330 AMLLLQKHHI GEVQSSVWPK TVMEQFENIL

References

[1]

"Complete sequence of Actinobacillus succinogenes 130Z." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Lowry S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Vieille C., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000746 Genomic DNA. Translation: ABR73878.1.
RefSeq	YP_001343813.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6VLN1.
Genome annotation databases
GeneID	5347770.
GenomeReviews	Gene locus Asuc_0503 in contig CP000746_GR.
KEGG	asu:Asuc_0503.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	LNDNLNG.
Family and domain databases
HAMAP	MF_00555. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II_cons-reg. IPR004618. AsnA. [Graphical view]
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
ProDom	PD024629. AsnA. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00669. asnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNA_ACTSZ

Accession

Primary (citable) accession number: A6VLN1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	August 21, 2007
Last modified:	November 3, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)