Reviewed, UniProtKB/Swiss-Prot A6VLE6 (ILVD_ACTSZ)

Names and origin

Protein names

Recommended name:
    Dihydroxy-acid dehydratase
      Short name=DAD
    EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	Asuc_0415

Organism

Actinobacillus succinogenes (strain ATCC 55618 / 130Z) [Complete proteome] [HAMAP]

Taxonomic identifier

339671 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	614 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential. HAMAP MF_00012
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the ilvD/edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 614	614	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000070948
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	195	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	A6VLE6-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 1A1E150F28F394BB	FASTA	614	65,954
	10 20 30 40 50 60 MPVLRSATST QGRNMAGARA LWRATGMKEN DFGKPIIAVV NSFTQFVPGH VHLKDMGQLV 70 80 90 100 110 120 AREIEKAGGV AKEFNTIAVD DGIAMGHGGM LYSLPSRDLI ADSVEYMVNA HCADAMVCIS 130 140 150 160 170 180 NCDKITPGML MAAMRLNIPT IFVSGGPMEA GKTKLSDKLI KLDLIDAMIQ SADANVSDAD 190 200 210 220 230 240 VDVIEKSACP TCGACSGMFT ANSMNCLTEA LGLSLPGNGS MLATHADRKE LFLEAGRKIV 250 260 270 280 290 300 EICKRHYEQD DYSVLPRSIA TFEAFENAMS LDIAMGGSSN TVLHLLAVAQ EAGVDFKMAD 310 320 330 340 350 360 IDRLSRVVPC LSKIAPNTNK YHMEDVHRAG GIMGIVGELD RAGLIHRKVK TILGLTMEEQ 370 380 390 400 410 420 LNQYDIIRNK DEKLNKFFRA GPAGIRTTEA FSQDCRWDTV DDDRANGCIR DRAHAVTEEG 430 440 450 460 470 480 GLAVLFGNIA EDGCIVKTAG VDESIWKFTG KAIVFESQED AVAGILGGKV KEGHVVVIRY 490 500 510 520 530 540 EGPKGGPGMQ EMLYPTSYLK SMGLGKKCAL LTDGRFSGGT SGLSIGHASP EAASGGAIGL 550 560 570 580 590 600 VNNDDIIEID IPNRAINLVL PDEELAVRRA AMEAKGARAW QPENRRREVS TALKIFGHFA 610 TSADKGAVRD KTKL

References

[1]

"Complete sequence of Actinobacillus succinogenes 130Z." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Lowry S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Vieille C., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000746 Genomic DNA. Translation: ABR73793.1.
RefSeq	YP_001343728.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6VLE6.
Genome annotation databases
GeneID	5349313.
GenomeReviews	Gene locus Asuc_0415 in contig CP000746_GR.
KEGG	asu:Asuc_0415.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0129.
HOGENOM	HBG671001.
OMA	SRKVPCL.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_ACTSZ

Accession

Primary (citable) accession number: A6VLE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	August 21, 2007
Last modified:	February 9, 2010
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)