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Protein

Phosphoenolpyruvate carboxykinase [ATP]

Gene

pckA

Organism
Actinobacillus succinogenes (strain ATCC 55618 / 130Z)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) through direct phosphoryl transfer between the nucleoside triphosphate and OAA (By similarity).By similarity

Catalytic activityi

ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotation1 PublicationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation1 Publication

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei64 – 641Substrate
Binding sitei152 – 1521ATP
Binding sitei205 – 2051Substrate
Metal bindingi211 – 2111ManganeseUniRule annotation1 Publication
Binding sitei211 – 2111ATPUniRule annotation
Binding sitei211 – 2111SubstrateUniRule annotation
Metal bindingi230 – 2301Manganese; via tele nitrogenUniRule annotation1 Publication
Binding sitei230 – 2301ATPUniRule annotation
Metal bindingi267 – 2671ManganeseUniRule annotation1 Publication
Binding sitei295 – 2951ATPUniRule annotation
Binding sitei331 – 3311ATPUniRule annotation
Binding sitei331 – 3311Substrate
Binding sitei344 – 3441ATP
Binding sitei453 – 4531ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi246 – 2549ATP
Nucleotide bindingi447 – 4482ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

ATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciASUC339671:GHDX-233-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [ATP]UniRule annotation (EC:4.1.1.49UniRule annotation)
Short name:
PCKUniRule annotation
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckAUniRule annotation
Ordered Locus Names:Asuc_0221
OrganismiActinobacillus succinogenes (strain ATCC 55618 / 130Z)
Taxonomic identifieri339671 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus
Proteomesi
  • UP000001114 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 538538Phosphoenolpyruvate carboxykinase [ATP]PRO_1000072371Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi339671.Asuc_0221.

Structurei

Secondary structure

1
538
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 1310Combined sources
Beta strandi22 – 254Combined sources
Helixi28 – 369Combined sources
Helixi42 – 443Combined sources
Beta strandi54 – 563Combined sources
Helixi66 – 683Combined sources
Beta strandi69 – 724Combined sources
Turni75 – 806Combined sources
Beta strandi86 – 883Combined sources
Beta strandi93 – 953Combined sources
Helixi97 – 11014Combined sources
Beta strandi112 – 1143Combined sources
Beta strandi116 – 1238Combined sources
Turni127 – 1293Combined sources
Beta strandi131 – 1388Combined sources
Helixi140 – 14910Combined sources
Helixi155 – 1595Combined sources
Beta strandi164 – 1707Combined sources
Turni176 – 1827Combined sources
Beta strandi184 – 1863Combined sources
Beta strandi188 – 1925Combined sources
Turni193 – 1964Combined sources
Beta strandi197 – 2026Combined sources
Helixi207 – 22317Combined sources
Beta strandi227 – 2304Combined sources
Beta strandi232 – 2365Combined sources
Beta strandi241 – 2455Combined sources
Helixi252 – 2554Combined sources
Beta strandi261 – 27111Combined sources
Beta strandi276 – 2794Combined sources
Beta strandi281 – 2866Combined sources
Turni292 – 2943Combined sources
Helixi296 – 3016Combined sources
Beta strandi307 – 3104Combined sources
Beta strandi331 – 3355Combined sources
Helixi336 – 3383Combined sources
Beta strandi339 – 3424Combined sources
Beta strandi345 – 3484Combined sources
Beta strandi352 – 3598Combined sources
Beta strandi368 – 3714Combined sources
Helixi374 – 38310Combined sources
Beta strandi385 – 3884Combined sources
Beta strandi400 – 4034Combined sources
Helixi405 – 4073Combined sources
Helixi409 – 4113Combined sources
Helixi416 – 43015Combined sources
Beta strandi433 – 4386Combined sources
Beta strandi445 – 4473Combined sources
Helixi450 – 46213Combined sources
Helixi464 – 4674Combined sources
Beta strandi470 – 4734Combined sources
Turni474 – 4774Combined sources
Beta strandi478 – 4825Combined sources
Turni489 – 4913Combined sources
Helixi494 – 4974Combined sources
Beta strandi498 – 5003Combined sources
Helixi501 – 51919Combined sources
Helixi520 – 5245Combined sources
Helixi526 – 5294Combined sources
Helixi530 – 5345Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YGGX-ray1.85A1-538[»]
1YLHX-ray1.70A1-538[»]
ProteinModelPortaliA6VKV4.
SMRiA6VKV4. Positions 3-537.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiA6VKV4.

Family & Domainsi

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [ATP] family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DJ1. Bacteria.
COG1866. LUCA.
HOGENOMiHOG000271471.
KOiK01610.
OMAiRFIVKEP.
OrthoDBiEOG6DG2RK.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00453. PEPCK_ATP.
InterProiIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
IPR015994. PEPCK_ATP_CS.
[Graphical view]
PfamiPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFiPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
TIGRFAMsiTIGR00224. pckA. 1 hit.
PROSITEiPS00532. PEPCK_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6VKV4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDLNKLVKE LNDLGLTDVK EIVYNPSYEQ LFEEETKPGL EGFDKGTLTT
60 70 80 90 100
LGAVAVDTGI FTGRSPKDKY IVCDETTKDT VWWNSEAAKN DNKPMTQETW
110 120 130 140 150
KSLRELVAKQ LSGKRLFVVE GYCGASEKHR IGVRMVTEVA WQAHFVKNMF
160 170 180 190 200
IRPTDEELKN FKADFTVLNG AKCTNPNWKE QGLNSENFVA FNITEGIQLI
210 220 230 240 250
GGTWYGGEMK KGMFSMMNYF LPLKGVASMH CSANVGKDGD VAIFFGLSGT
260 270 280 290 300
GKTTLSTDPK RQLIGDDEHG WDESGVFNFE GGCYAKTINL SQENEPDIYG
310 320 330 340 350
AIRRDALLEN VVVRADGSVD FDDGSKTENT RVSYPIYHID NIVRPVSKAG
360 370 380 390 400
HATKVIFLTA DAFGVLPPVS KLTPEQTEYY FLSGFTAKLA GTERGVTEPT
410 420 430 440 450
PTFSACFGAA FLSLHPIQYA DVLVERMKAS GAEAYLVNTG WNGTGKRISI
460 470 480 490 500
KDTRGIIDAI LDGSIEKAEM GELPIFNLAI PKALPGVDPA ILDPRDTYAD
510 520 530
KAQWQVKAED LANRFVKNFV KYTANPEAAK LVGAGPKA
Length:538
Mass (Da):59,154
Last modified:August 21, 2007 - v1
Checksum:i40205AB2A8D79871
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000746 Genomic DNA. Translation: ABR73601.1.
RefSeqiWP_011978877.1. NC_009655.1.

Genome annotation databases

EnsemblBacteriaiABR73601; ABR73601; Asuc_0221.
KEGGiasu:Asuc_0221.
PATRICi20758626. VBIActSuc117883_0243.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000746 Genomic DNA. Translation: ABR73601.1.
RefSeqiWP_011978877.1. NC_009655.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YGGX-ray1.85A1-538[»]
1YLHX-ray1.70A1-538[»]
ProteinModelPortaliA6VKV4.
SMRiA6VKV4. Positions 3-537.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi339671.Asuc_0221.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABR73601; ABR73601; Asuc_0221.
KEGGiasu:Asuc_0221.
PATRICi20758626. VBIActSuc117883_0243.

Phylogenomic databases

eggNOGiENOG4105DJ1. Bacteria.
COG1866. LUCA.
HOGENOMiHOG000271471.
KOiK01610.
OMAiRFIVKEP.
OrthoDBiEOG6DG2RK.

Enzyme and pathway databases

UniPathwayiUPA00138.
BioCyciASUC339671:GHDX-233-MONOMER.

Miscellaneous databases

EvolutionaryTraceiA6VKV4.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00453. PEPCK_ATP.
InterProiIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
IPR015994. PEPCK_ATP_CS.
[Graphical view]
PfamiPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFiPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
TIGRFAMsiTIGR00224. pckA. 1 hit.
PROSITEiPS00532. PEPCK_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A genomic perspective on the potential of Actinobacillus succinogenes for industrial succinate production."
    McKinlay J.B., Laivenieks M., Schindler B.D., McKinlay A.A., Siddaramappa S., Challacombe J.F., Lowry S.R., Clum A., Lapidus A.L., Burkhart K.B., Harkins V., Vieille C.
    BMC Genomics 11:680-680(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 55618 / 130Z.
  2. "Structure of PEP carboxykinase from the succinate-producing Actinobacillus succinogenes: a new conserved active-site motif."
    Leduc Y.A., Prasad L., Laivenieks M., Zeikus J.G., Delbaere L.T.
    Acta Crystallogr. D 61:903-912(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) IN COMPLEX WITH MANGANESE, SUBSTRATE AND ATP ANALOGS, COFACTOR.

Entry informationi

Entry nameiPCKA_ACTSZ
AccessioniPrimary (citable) accession number: A6VKV4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: August 21, 2007
Last modified: November 11, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.