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Protein

Phosphoenolpyruvate carboxykinase [ATP]

Gene

pckA

Organism
Actinobacillus succinogenes (strain ATCC 55618 / 130Z)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) through direct phosphoryl transfer between the nucleoside triphosphate and OAA (By similarity).By similarity

Catalytic activityi

ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotation1 PublicationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation1 Publication

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei64Substrate1
Binding sitei152ATP1
Binding sitei205Substrate1
Metal bindingi211ManganeseUniRule annotation1 Publication1
Binding sitei211ATPUniRule annotation1
Binding sitei211SubstrateUniRule annotation1
Metal bindingi230Manganese; via tele nitrogenUniRule annotation1 Publication1
Binding sitei230ATPUniRule annotation1
Metal bindingi267ManganeseUniRule annotation1 Publication1
Binding sitei295ATPUniRule annotation1
Binding sitei331ATPUniRule annotation1
Binding sitei331Substrate1
Binding sitei344ATP1
Binding sitei453ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi246 – 254ATP9
Nucleotide bindingi447 – 448ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

ATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [ATP]UniRule annotation (EC:4.1.1.49UniRule annotation)
Short name:
PCKUniRule annotation
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckAUniRule annotation
Ordered Locus Names:Asuc_0221
OrganismiActinobacillus succinogenes (strain ATCC 55618 / 130Z)
Taxonomic identifieri339671 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus
Proteomesi
  • UP000001114 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000723711 – 538Phosphoenolpyruvate carboxykinase [ATP]Add BLAST538

Proteomic databases

PRIDEiA6VKV4.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi339671.Asuc_0221.

Structurei

Secondary structure

1538
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 13Combined sources10
Beta strandi22 – 25Combined sources4
Helixi28 – 36Combined sources9
Helixi42 – 44Combined sources3
Beta strandi54 – 56Combined sources3
Helixi66 – 68Combined sources3
Beta strandi69 – 72Combined sources4
Turni75 – 80Combined sources6
Beta strandi86 – 88Combined sources3
Beta strandi93 – 95Combined sources3
Helixi97 – 110Combined sources14
Beta strandi112 – 114Combined sources3
Beta strandi116 – 123Combined sources8
Turni127 – 129Combined sources3
Beta strandi131 – 138Combined sources8
Helixi140 – 149Combined sources10
Helixi155 – 159Combined sources5
Beta strandi164 – 170Combined sources7
Turni176 – 182Combined sources7
Beta strandi184 – 186Combined sources3
Beta strandi188 – 192Combined sources5
Turni193 – 196Combined sources4
Beta strandi197 – 202Combined sources6
Helixi207 – 223Combined sources17
Beta strandi227 – 230Combined sources4
Beta strandi232 – 236Combined sources5
Beta strandi241 – 245Combined sources5
Helixi252 – 255Combined sources4
Beta strandi261 – 271Combined sources11
Beta strandi276 – 279Combined sources4
Beta strandi281 – 286Combined sources6
Turni292 – 294Combined sources3
Helixi296 – 301Combined sources6
Beta strandi307 – 310Combined sources4
Beta strandi331 – 335Combined sources5
Helixi336 – 338Combined sources3
Beta strandi339 – 342Combined sources4
Beta strandi345 – 348Combined sources4
Beta strandi352 – 359Combined sources8
Beta strandi368 – 371Combined sources4
Helixi374 – 383Combined sources10
Beta strandi385 – 388Combined sources4
Beta strandi400 – 403Combined sources4
Helixi405 – 407Combined sources3
Helixi409 – 411Combined sources3
Helixi416 – 430Combined sources15
Beta strandi433 – 438Combined sources6
Beta strandi445 – 447Combined sources3
Helixi450 – 462Combined sources13
Helixi464 – 467Combined sources4
Beta strandi470 – 473Combined sources4
Turni474 – 477Combined sources4
Beta strandi478 – 482Combined sources5
Turni489 – 491Combined sources3
Helixi494 – 497Combined sources4
Beta strandi498 – 500Combined sources3
Helixi501 – 519Combined sources19
Helixi520 – 524Combined sources5
Helixi526 – 529Combined sources4
Helixi530 – 534Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YGGX-ray1.85A1-538[»]
1YLHX-ray1.70A1-538[»]
ProteinModelPortaliA6VKV4.
SMRiA6VKV4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiA6VKV4.

Family & Domainsi

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [ATP] family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DJ1. Bacteria.
COG1866. LUCA.
HOGENOMiHOG000271471.
KOiK01610.
OMAiRFIVKEP.
OrthoDBiPOG091H03CD.

Family and domain databases

CDDicd00484. PEPCK_ATP. 1 hit.
Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00453. PEPCK_ATP. 1 hit.
InterProiIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
IPR015994. PEPCK_ATP_CS.
[Graphical view]
PfamiPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFiPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
TIGRFAMsiTIGR00224. pckA. 1 hit.
PROSITEiPS00532. PEPCK_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6VKV4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDLNKLVKE LNDLGLTDVK EIVYNPSYEQ LFEEETKPGL EGFDKGTLTT
60 70 80 90 100
LGAVAVDTGI FTGRSPKDKY IVCDETTKDT VWWNSEAAKN DNKPMTQETW
110 120 130 140 150
KSLRELVAKQ LSGKRLFVVE GYCGASEKHR IGVRMVTEVA WQAHFVKNMF
160 170 180 190 200
IRPTDEELKN FKADFTVLNG AKCTNPNWKE QGLNSENFVA FNITEGIQLI
210 220 230 240 250
GGTWYGGEMK KGMFSMMNYF LPLKGVASMH CSANVGKDGD VAIFFGLSGT
260 270 280 290 300
GKTTLSTDPK RQLIGDDEHG WDESGVFNFE GGCYAKTINL SQENEPDIYG
310 320 330 340 350
AIRRDALLEN VVVRADGSVD FDDGSKTENT RVSYPIYHID NIVRPVSKAG
360 370 380 390 400
HATKVIFLTA DAFGVLPPVS KLTPEQTEYY FLSGFTAKLA GTERGVTEPT
410 420 430 440 450
PTFSACFGAA FLSLHPIQYA DVLVERMKAS GAEAYLVNTG WNGTGKRISI
460 470 480 490 500
KDTRGIIDAI LDGSIEKAEM GELPIFNLAI PKALPGVDPA ILDPRDTYAD
510 520 530
KAQWQVKAED LANRFVKNFV KYTANPEAAK LVGAGPKA
Length:538
Mass (Da):59,154
Last modified:August 21, 2007 - v1
Checksum:i40205AB2A8D79871
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000746 Genomic DNA. Translation: ABR73601.1.
RefSeqiWP_011978877.1. NC_009655.1.

Genome annotation databases

EnsemblBacteriaiABR73601; ABR73601; Asuc_0221.
KEGGiasu:Asuc_0221.
PATRICi20758626. VBIActSuc117883_0243.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000746 Genomic DNA. Translation: ABR73601.1.
RefSeqiWP_011978877.1. NC_009655.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YGGX-ray1.85A1-538[»]
1YLHX-ray1.70A1-538[»]
ProteinModelPortaliA6VKV4.
SMRiA6VKV4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi339671.Asuc_0221.

Proteomic databases

PRIDEiA6VKV4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABR73601; ABR73601; Asuc_0221.
KEGGiasu:Asuc_0221.
PATRICi20758626. VBIActSuc117883_0243.

Phylogenomic databases

eggNOGiENOG4105DJ1. Bacteria.
COG1866. LUCA.
HOGENOMiHOG000271471.
KOiK01610.
OMAiRFIVKEP.
OrthoDBiPOG091H03CD.

Enzyme and pathway databases

UniPathwayiUPA00138.

Miscellaneous databases

EvolutionaryTraceiA6VKV4.

Family and domain databases

CDDicd00484. PEPCK_ATP. 1 hit.
Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00453. PEPCK_ATP. 1 hit.
InterProiIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
IPR015994. PEPCK_ATP_CS.
[Graphical view]
PfamiPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFiPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
TIGRFAMsiTIGR00224. pckA. 1 hit.
PROSITEiPS00532. PEPCK_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCKA_ACTSZ
AccessioniPrimary (citable) accession number: A6VKV4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: August 21, 2007
Last modified: November 2, 2016
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.