Reviewed, UniProtKB/Swiss-Prot A6VKR5 (SYP_ACTSZ)

Names and origin

Protein names

Recommended name:
    Prolyl-tRNA synthetase
    EC=6.1.1.15
Alternative name(s):
    Proline--tRNA ligase
      Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	Asuc_0182

Organism

Actinobacillus succinogenes (strain ATCC 55618 / 130Z) [Complete proteome] [HAMAP]

Taxonomic identifier

339671 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	571 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity.
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01569
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP proline-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 571	571	Prolyl-tRNA synthetase HAMAP MF_01569		PRO_1000073586

Sequences

	Sequence		Length	Mass (Da)
	A6VKR5-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 9FE9669149C939EB	FASTA	571	63,561
	10 20 30 40 50 60 MRTSQYLFST LKETPNDAQV VSHQLMLRAG MIRSMASGLY NWLPTGVKVL KKVENIIREE 70 80 90 100 110 120 MNKGGAIEVL MPVVQSAELW QESGRWEQYG PELLRFSDRG NRDFVLGPTH EEVITDLVRR 130 140 150 160 170 180 EVSSYKQLPL NLYQIQTKFR DEVRPRFGVM RSREFVMKDA YSFHTTQESL QQTYEVMYQV 190 200 210 220 230 240 YTNIFTRLGL DFRAVQADTG SIGGSASHEF QVLASSGEDD VVFSTESDFA ANIELAEAIA 250 260 270 280 290 300 VGERQAPGKA MELVDTPNAK TIAELVEQFN LPIEKTVKTL IVKGATEDAP LVALIIRGDH 310 320 330 340 350 360 ELNEIKAQKH PLVADPLEFA DETEIKAKIG AGVGSLGPVN LNIPAIIDRS VALMSDFGAG 370 380 390 400 410 420 ANIDGKHYFN INWERDAAMP EAFDLRNVAE GDPSPDGKGT LQIKRGIEVG HIFQLGKKYS 430 440 450 460 470 480 EAMNATVQGE DGKPLVMTMG CYGIGVTRVV AAAIEQHHDE RGIIWPTDEI APFTVAIVPM 490 500 510 520 530 540 NMHKSESVRA FAEELYQTLK AQGVDVIFDD RKERPGVMFA DMELIGVPHM VVIGEKNLDK 550 560 570 GEIEYKNRRN GEKQMIAKDR LLAFLAENVK A

References

[1]

"Complete sequence of Actinobacillus succinogenes 130Z." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Lowry S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Vieille C., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000746 Genomic DNA. Translation: ABR73562.1.
RefSeq	YP_001343497.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6VKR5.
Genome annotation databases
GeneID	5347977.
GenomeReviews	Gene locus Asuc_0182 in contig CP000746_GR.
KEGG	asu:Asuc_0182.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VVSHQLM.
Family and domain databases
HAMAP	MF_01569. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR004500. Pro-tRNA-synth_IIa_bac. IPR002316. Pro-tRNA-synth_IIa_cons-reg. IPR007214. YbaK/aa-tRNA-synth-assoc-reg. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.90.960.10. YbaK/aa-tRNA-synth-assoc-reg. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF04073. YbaK. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
TIGRFAMs	TIGR00409. proS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_ACTSZ

Accession

Primary (citable) accession number: A6VKR5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	August 21, 2007
Last modified:	November 3, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)