Reviewed, UniProtKB/Swiss-Prot A6VKD6 (SYA_ACTSZ)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Asuc_0052

Organism

Actinobacillus succinogenes (strain ATCC 55618 / 130Z) [Complete proteome] [HAMAP]

Taxonomic identifier

339671 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	874 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	The C-terminal C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Domain	Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 874	874	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000347479
Regions
	Zinc finger	178 – 191	14	C2H2-type HAMAP MF_00036

Sequences

	Sequence		Length	Mass (Da)
	A6VKD6-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: BD1EF118813B3295	FASTA	874	96,045
	10 20 30 40 50 60 MKTTAEIRQS FLDFFHSKGH QVVESSSLVP ENDPTLLFTN AGMNQFKDVF LGMDKRPYSR 70 80 90 100 110 120 ATTAQRCVRA GGKHNDLENV GYTARHHTFF EMMGNFSFGD YFKYDAINFA WEFLTSPKWL 130 140 150 160 170 180 ALPKEKLYVT VYETDDEAYD IWNKEVGVPQ EHIIRIGDNK GAPYASDNFW AMGDTGPCGP 190 200 210 220 230 240 CTEIFYDHGD HIWGGLPGTP EEDGDRYIEI WNIVFMQFNR LADGTMEKLP KPSVDTGMGL 250 260 270 280 290 300 ERMTAVLQHV NSNYEIDIFQ TLIKKVAEIV GTTDLGNKSL RVIADHIRSC AYLIADGVIP 310 320 330 340 350 360 SNEGRGYVLR RIIRRAVRHG HLLGAKETFF YKLVPTLIEV MAEAGKQVKE KQANVEKLLR 370 380 390 400 410 420 LEEEQFARTL ERGLILLDAE LANVKNGVLS GEVAFKLYDT YGFPLDLTAD VCRERNIAVD 430 440 450 460 470 480 ENGFDKEMEA QRLRAQSASN FGMDYTSIIR VDGETKFEGY TNVESLAKVT ALFRDGKAVE 490 500 510 520 530 540 SVSAGQSAVV ILENTPFYAE MGGQIGDSGM LTANGLHFDV KDTQKYGQVF GHIGELTQGT 550 560 570 580 590 600 LSVGQIVNAQ VDAQRRQYTK LNHSATHLLH AALRQVLGEH VAQKGSLVSD VGLRFDFVQP 610 620 630 640 650 660 EAVTKEQIME IERLVNFHIR ANHPVLTEEM GVAEAKSKGA IALFGEKYGE TVRVVTMSPF 670 680 690 700 710 720 SIELCGGLHV ERTGEIGLLK IISEGAVAAG IRRVEAVTGE EAINWLFNQQ TILAQSADLL 730 740 750 760 770 780 KSDIPSLPEK IIQLQDKVKK TEKELQQLKE KAAMQAGSDL AKSAVKINGV SVVTHQLDGA 790 800 810 820 830 840 DAKVLRVMVD DLKNQLGSAV IALGSASGGK VNLIAGVTAD LTAKVKAGEL VNVMAQQVGG 850 860 870 KGGGRPDMAM AGGSQPENLS AALIVASDWL KTMI

References

[1]

"Complete sequence of Actinobacillus succinogenes 130Z." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Lowry S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Vieille C., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000746 Genomic DNA. Translation: ABR73433.1.
RefSeq	YP_001343368.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6VKD6.
Genome annotation databases
GeneID	5348995.
GenomeReviews	Gene locus Asuc_0052 in contig CP000746_GR.
KEGG	asu:Asuc_0052.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	GEFVIKL.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018165. Ala-tRNA-synth_IIc_cons-reg. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_ACTSZ

Accession

Primary (citable) accession number: A6VKD6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	August 21, 2007
Last modified:	November 3, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)