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Protein

Diphthine synthase

Gene

dphB

Organism
Methanococcus maripaludis (strain C7 / ATCC BAA-1331)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the trimethylation of the amino group of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine. The three successive methylation reactions represent the second step of diphthamide biosynthesis.UniRule annotation

Catalytic activityi

3 S-adenosyl-L-methionine + 2-((3S)-3-carboxy-3-aminopropyl)-L-histidine-[translation elongation factor 2] = 3 S-adenosyl-L-homocysteine + diphthine-[translation elongation factor 2].UniRule annotation

Pathwayi: peptidyl-diphthamide biosynthesis

This protein is involved in the pathway peptidyl-diphthamide biosynthesis, which is part of Protein modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidyl-diphthamide biosynthesis and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei9 – 91S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei85 – 851S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation
Binding sitei88 – 881S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei164 – 1641S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation
Binding sitei207 – 2071S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation
Binding sitei232 – 2321S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMMAR426368:GHHL-1655-MONOMER.
UniPathwayiUPA00559.

Names & Taxonomyi

Protein namesi
Recommended name:
Diphthine synthaseUniRule annotation (EC:2.1.1.98UniRule annotation)
Alternative name(s):
Diphthamide biosynthesis methyltransferaseUniRule annotation
Gene namesi
Name:dphBUniRule annotation
Ordered Locus Names:MmarC7_1609
OrganismiMethanococcus maripaludis (strain C7 / ATCC BAA-1331)
Taxonomic identifieri426368 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus
Proteomesi
  • UP000002298 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 255255Diphthine synthasePRO_1000064822Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi426368.MmarC7_1609.

Structurei

3D structure databases

ProteinModelPortaliA6VJP1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni113 – 1142S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the diphthine synthase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04161. Archaea.
COG1798. LUCA.
HOGENOMiHOG000205302.
KOiK20215.
OMAiMTANEGL.

Family and domain databases

CDDicd11647. Diphthine_synthase. 1 hit.
Gene3Di3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
HAMAPiMF_01084. Diphthine_synth. 1 hit.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR004551. Dphthn_synthase.
[Graphical view]
PANTHERiPTHR10882:SF0. PTHR10882:SF0. 1 hit.
PfamiPF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF036432. Diphthine_synth. 1 hit.
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR00522. dph5. 1 hit.

Sequencei

Sequence statusi: Complete.

A6VJP1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVMAGLGLY DERDVTLKTL DFAKKVDKIY AEFYTAILTG TSMEKIENTL
60 70 80 90 100
QKPITVLDRE KVEYETNKLI EEAVDKDIMF LTAGDPMVAT THVDIAVEAR
110 120 130 140 150
KKGIEVVIIN APSIYSAIGI TGLQLYKFGK TTSVVFPEPN YFPETPYDVI
160 170 180 190 200
KDNLKLGYHT LCLLDIQADK ERFMTANEGL DALLKIEEKR NENVISGETY
210 220 230 240 250
AAVVARAGSI KPGLYYGKIK DLINYDFGTP LHCVIIPGKL HFMEEDALKY

LFENI
Length:255
Mass (Da):28,662
Last modified:August 21, 2007 - v1
Checksum:i6685850A11AF43B0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000745 Genomic DNA. Translation: ABR66667.1.
RefSeqiWP_012068127.1. NC_009637.1.

Genome annotation databases

EnsemblBacteriaiABR66667; ABR66667; MmarC7_1609.
GeneIDi5328268.
KEGGimmz:MmarC7_1609.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000745 Genomic DNA. Translation: ABR66667.1.
RefSeqiWP_012068127.1. NC_009637.1.

3D structure databases

ProteinModelPortaliA6VJP1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi426368.MmarC7_1609.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABR66667; ABR66667; MmarC7_1609.
GeneIDi5328268.
KEGGimmz:MmarC7_1609.

Phylogenomic databases

eggNOGiarCOG04161. Archaea.
COG1798. LUCA.
HOGENOMiHOG000205302.
KOiK20215.
OMAiMTANEGL.

Enzyme and pathway databases

UniPathwayiUPA00559.
BioCyciMMAR426368:GHHL-1655-MONOMER.

Family and domain databases

CDDicd11647. Diphthine_synthase. 1 hit.
Gene3Di3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
HAMAPiMF_01084. Diphthine_synth. 1 hit.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR004551. Dphthn_synthase.
[Graphical view]
PANTHERiPTHR10882:SF0. PTHR10882:SF0. 1 hit.
PfamiPF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF036432. Diphthine_synth. 1 hit.
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR00522. dph5. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDPHB_METM7
AccessioniPrimary (citable) accession number: A6VJP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: August 21, 2007
Last modified: September 7, 2016
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.