A6VJ27 (PUR2_METM7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylamine--glycine ligase
EC=6.3.4.13

Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	MmarC7_1390

Organism

Methanococcus maripaludis (strain C7 / ATCC BAA-1331) [Complete proteome] [HAMAP]

Taxonomic identifier

426368 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus › Methanococcus maripaludis

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway purine nucleobase biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 444	444	Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138		PRO_1000018825
Regions
	Domain	109 – 324	216	ATP-grasp
	Nucleotide binding	140 – 202	63	ATP By similarity
Sites
	Metal binding	282	1	Magnesium or manganese 1 By similarity
	Metal binding	294	1	Magnesium or manganese 1 By similarity
	Metal binding	294	1	Magnesium or manganese 2 By similarity
	Metal binding	296	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6VJ27 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 59AAC5CEBD0A583F	FASTA	444	48,742
	10 20 30 40 50 60 MKVLLIGGGA REHAIAMALK KNELVELYTL MKNKNPGIYA LSEEVSFNSE TDVPAIKEFA 70 80 90 100 110 120 EKIKPEIAVI GPESPLGVGA ADLLEEMGIP TVGPKKLPAQ IETSKEFMRN LFKKYEIDGS 130 140 150 160 170 180 LKYAAFNEYG NEIESFIDEM TSLGKDVVVK PAGLTGGKGV KVVGEQLKDN DEAKIYAKEV 190 200 210 220 230 240 FDKSIGGGKI IIEEKLVGVE FTLHGFVDGE NITFMPAVQD HPHAYNNDEG PITGGMGSYS 250 260 270 280 290 300 CPNHGLPFIS EDMLDRAEKI MEKTVNSINL EVGPYKGFLY GQFMLTADGP KIIEYNARFG 310 320 330 340 350 360 DPEAMNLLPI LKTDFLDVCF AIAEGKLDKM NIEFENKATV CKYVVPNGYP IDPVKNKELA 370 380 390 400 410 420 VAEKAIEDAG AILFYASINE ENGKLYITGS RSAAVVGISE NIEEAEKIAQ KAIENFKGEV 430 440 YYRSDIGTLD LIKKRIERVK KLAK

References

[1]

"Complete sequence of Methanococcus maripaludis C7." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I., Sieprawska-Lupa M., Whitman W.B., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C7 / ATCC BAA-1331.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000745 Genomic DNA. Translation: ABR66453.1.
RefSeq	YP_001330604.1. NC_009637.1.
3D structure databases
ProteinModelPortal	A6VJ27.
ModBase	Search...
Protein-protein interaction databases
STRING	426368.MmarC7_1390.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABR66453; ABR66453; MmarC7_1390.
GeneID	5328380.
KEGG	mmz:MmarC7_1390.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0151.
HOGENOM	HOG000033464.
KO	K01945.
OMA	MGAYTPL.
ProtClustDB	PRK00885.
Enzyme and pathway databases
BioCyc	MMAR426368:GHHL-1437-MONOMER.
UniPathway	UPA00074; UER00125.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 1 hit. 3.40.50.20. 1 hit. 3.90.600.10. 1 hit.
HAMAP	MF_00138. GARS.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR016185. PreATP-grasp_dom. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N. IPR011054. Rudment_hybrid_motif. [Graphical view]
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit. SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_METM7

Accession

Primary (citable) accession number: A6VJ27

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 21, 2007
Last modified:	May 1, 2013
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program