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Protein

Phosphoribosylamine--glycine ligase

Gene

purD

Organism
Methanococcus maripaludis (strain C7 / ATCC BAA-1331)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (purF)
  2. Phosphoribosylamine--glycine ligase (purD)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi282Magnesium or manganese 1UniRule annotation1
Metal bindingi294Magnesium or manganese 1UniRule annotation1
Metal bindingi294Magnesium or manganese 2UniRule annotation1
Metal bindingi296Magnesium or manganese 2UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi140 – 202ATPUniRule annotationAdd BLAST63

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00125.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylamine--glycine ligaseUniRule annotation (EC:6.3.4.13UniRule annotation)
Alternative name(s):
GARSUniRule annotation
Glycinamide ribonucleotide synthetaseUniRule annotation
Phosphoribosylglycinamide synthetaseUniRule annotation
Gene namesi
Name:purDUniRule annotation
Ordered Locus Names:MmarC7_1390
OrganismiMethanococcus maripaludis (strain C7 / ATCC BAA-1331)
Taxonomic identifieri426368 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus
Proteomesi
  • UP000002298 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000188251 – 444Phosphoribosylamine--glycine ligaseAdd BLAST444

Interactioni

Protein-protein interaction databases

STRINGi426368.MmarC7_1390.

Structurei

3D structure databases

ProteinModelPortaliA6VJ27.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini109 – 324ATP-graspUniRule annotationAdd BLAST216

Sequence similaritiesi

Belongs to the GARS family.UniRule annotation
Contains 1 ATP-grasp domain.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04415. Archaea.
COG0151. LUCA.
HOGENOMiHOG000033464.
KOiK01945.
OMAiFGSAQDH.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPiMF_00138. GARS. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SMARTiSM01210. GARS_C. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR00877. purD. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6VJ27-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVLLIGGGA REHAIAMALK KNELVELYTL MKNKNPGIYA LSEEVSFNSE
60 70 80 90 100
TDVPAIKEFA EKIKPEIAVI GPESPLGVGA ADLLEEMGIP TVGPKKLPAQ
110 120 130 140 150
IETSKEFMRN LFKKYEIDGS LKYAAFNEYG NEIESFIDEM TSLGKDVVVK
160 170 180 190 200
PAGLTGGKGV KVVGEQLKDN DEAKIYAKEV FDKSIGGGKI IIEEKLVGVE
210 220 230 240 250
FTLHGFVDGE NITFMPAVQD HPHAYNNDEG PITGGMGSYS CPNHGLPFIS
260 270 280 290 300
EDMLDRAEKI MEKTVNSINL EVGPYKGFLY GQFMLTADGP KIIEYNARFG
310 320 330 340 350
DPEAMNLLPI LKTDFLDVCF AIAEGKLDKM NIEFENKATV CKYVVPNGYP
360 370 380 390 400
IDPVKNKELA VAEKAIEDAG AILFYASINE ENGKLYITGS RSAAVVGISE
410 420 430 440
NIEEAEKIAQ KAIENFKGEV YYRSDIGTLD LIKKRIERVK KLAK
Length:444
Mass (Da):48,742
Last modified:August 21, 2007 - v1
Checksum:i59AAC5CEBD0A583F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000745 Genomic DNA. Translation: ABR66453.1.
RefSeqiWP_012067919.1. NC_009637.1.

Genome annotation databases

EnsemblBacteriaiABR66453; ABR66453; MmarC7_1390.
GeneIDi5328380.
KEGGimmz:MmarC7_1390.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000745 Genomic DNA. Translation: ABR66453.1.
RefSeqiWP_012067919.1. NC_009637.1.

3D structure databases

ProteinModelPortaliA6VJ27.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi426368.MmarC7_1390.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABR66453; ABR66453; MmarC7_1390.
GeneIDi5328380.
KEGGimmz:MmarC7_1390.

Phylogenomic databases

eggNOGiarCOG04415. Archaea.
COG0151. LUCA.
HOGENOMiHOG000033464.
KOiK01945.
OMAiFGSAQDH.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00125.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPiMF_00138. GARS. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SMARTiSM01210. GARS_C. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR00877. purD. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPUR2_METM7
AccessioniPrimary (citable) accession number: A6VJ27
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 21, 2007
Last modified: November 2, 2016
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.