A6VIV7 (ILVD_METM7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	MmarC7_1320

Organism

Methanococcus maripaludis (strain C7 / ATCC BAA-1331) [Complete proteome] [HAMAP]

Taxonomic identifier

426368 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	550 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 550	550	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000001007
Sites
	Metal binding	119	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	191	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	A6VIV7 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: E71E4E02A27D9AC9	FASTA	550	58,524
	10 20 30 40 50 60 MISDNVKKGV IRSPNRALLK ACGYSDEDME KPFIGVVNSF TEVVPGHIHL KTLSEAVKHG 70 80 90 100 110 120 VYANGGTPFE FNTIGICDGI AMGHEGMKYS LPSREIIADA VESMARAHGF DGLVLIPTCD 130 140 150 160 170 180 KIVPGMIMGA LRLNIPFIVV TGGPMLPGEF QGKKCELISL FEGVGEYQVG KITEEELKSI 190 200 210 220 230 240 EECACPGAGS CAGLYTANSM ACLTEALGLS LPMCATIHAV DAQKVRIAKK TGSKIVDLVK 250 260 270 280 290 300 EDVKPTDILT KEAFENAILV DLALGGSTNT TLHIPAIANE IENKFITLDD FDRLSNEVPH 310 320 330 340 350 360 IASIKPGGEH YMIDLHNAGG IPAVLNVLKG KIRDTKTVDG RSTLEIAESV KYINYDVIRK 370 380 390 400 410 420 VEAPVHETAG LRVLKGNLAP NGCVVKIGAV NPKMYKHDGP AKVYNSEDEA ISAILGGKIV 430 440 450 460 470 480 EGDVIVIRYE GPSGGPGMRE MLSPTSAICG MGLDDSVALI TDGRFSGGSR GPCIGHVSPE 490 500 510 520 530 540 AAAGGVIAAI ENGDIIKIDM IGKEINVDLD ESVIKERLSK LDEFEPKIKK GYLSRYSRLV 550 SSADEGAVLK

References

[1]

"Complete sequence of Methanococcus maripaludis C7." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I., Sieprawska-Lupa M., Whitman W.B., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C7 / ATCC BAA-1331.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000745 Genomic DNA. Translation: ABR66383.1.
RefSeq	YP_001330534.1. NC_009637.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6VIV7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5328578.
GenomeReviews	Gene locus MmarC7_1320 in contig CP000745_GR.
KEGG	mmz:MmarC7_1320.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04340.
HOGENOM	HBG671001.
OMA	SQDCRWP.
ProtClustDB	PRK00911.
Enzyme and pathway databases
BioCyc	MMAR426368:MMARC7_1320-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_METM7

Accession

Primary (citable) accession number: A6VIV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 21, 2007
Last modified:	November 16, 2011
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program