Reviewed, UniProtKB/Swiss-Prot A6VIH3 (RIFK_METM7)

Names and origin

Protein names

Recommended name:
    Riboflavin kinase
      Short name=RFK
    EC=2.7.1.161
Alternative name(s):
    CTP:riboflavin 5'-phosphotransferase
    CTP-dependent riboflavin kinase
    Flavokinase

Gene names

Name:	rfk
Ordered Locus Names:	MmarC7_1183

Organism

Methanococcus maripaludis (strain C7 / ATCC BAA-1331) [Complete proteome] [HAMAP]

Taxonomic identifier

426368 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	127 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) By similarity. HAMAP MF_01285
Catalytic activity	CTP + riboflavin = CDP + FMN. HAMAP MF_01285
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01285
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP MF_01285
Sequence similarities	Belongs to the archaeal riboflavin kinase family.

Ontologies

Keywords
Ligand	FMN Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	FMN biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: HAMAP riboflavin kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 127	127	Riboflavin kinase HAMAP MF_01285		PRO_0000322073
Regions
	Nucleotide binding	10 – 15	6	CDP By similarity
	Nucleotide binding	109 – 112	4	CDP By similarity
Sites
	Metal binding	39	1	Magnesium By similarity
	Metal binding	41	1	Magnesium By similarity
	Binding site	96	1	FMN By similarity
	Binding site	104	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6VIH3-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 1C457EADBBF6F6A9	FASTA	127	14,247
	10 20 30 40 50 60 MEIFGNVVSG LGEGRFFVGL TPYKNKFKEL TGFTPFEGTL NVKLKHNFNL DEFNPIEFDG 70 80 90 100 110 120 FEIDGKKYFG GKVLLVKLFD KSGHSINCAI VAPKKTDHSK KTLELIAPVH LRKFLSLNNS DVVKIVI

References

[1]

"Complete sequence of Methanococcus maripaludis C7." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I., Sieprawska-Lupa M., Whitman W.B., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000745 Genomic DNA. Translation: ABR66249.1.
RefSeq	YP_001330400.1.
3D structure databases
SMR	A6VIH3. Positions 1-127.
ModBase	Search...
Protein-protein interaction databases
STRING	A6VIH3.
Genome annotation databases
GeneID	5328868.
GenomeReviews	Gene locus MmarC7_1183 in contig CP000745_GR.
KEGG	mmz:MmarC7_1183.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG11680.
HOGENOM	HBG553349.
OMA	KTDHSKK.
Family and domain databases
HAMAP	MF_01285. Riboflavin_kinase. [Tree]
InterPro	IPR002834. Riboflavin_kinase_arc. [Graphical view]
Pfam	PF01982. CTP-dep_RFKase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIFK_METM7

Accession

Primary (citable) accession number: A6VIH3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	August 21, 2007
Last modified:	January 19, 2010
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)